Mycological and Serological Study of Invasive Aspergillosis in A Tertiary Care Hospital

Year 2018, Volume: 08 Issue: 01, 8 - 12, 15.03.2018

Sumanpreet Kaur Veenu Gupta Deepinder Kaur Chhina Akashdeep Singh Daaman Sharma

https://doi.org/10.5799/jmid.394584

Cited By: 1

Abstract

Objectives: Invasive
aspergillosis (IA) has emerged as a major life threatening infection in high
risk patients. Recent advances have led to early diagnosis of IA by detecting Aspergillus antigens in serum
particularly Galactomannan (GM). Objective of the study was to correlate fungal
smear, culture and Galactomannan assay in patients with suspected IA and to
categorize them into “proven,” “probable,” and “possible,” cases as per
European Organization for Research and Treatment of Cancer and Mycoses study
group case definitions (EORTC/MSG) criteria.

Methods: The prospective
study was conducted over a period of one year in the department of
Microbiology, Dayanand Medical College and Hospital, Ludhiana. A total of 319
patients with suspicion of IA were included in the study. GM antigen detection
test was done from serum samples. KOH mount was done and samples were
inoculated on Sabouraud’s dextrose agar (SDA). Growth obtained was analyzed as
per standard protocol.

Results: GM assay positivity
among suspected patients of IA was 47%. The mean age group affected was 51.8
years (51.8±15.2) and majority was males (78.1%). The patients were categorized
as Proven IA (0%), Probable IA (52.7%), Possible IA (41.4%) and No IA (5.9%). Aspergillus flavus was the most common
isolate (77.4%)

Conclusion: The study
demonstrated that serological tests have an edge over routine smear and culture
for the diagnosis of invasive aspergillosis. Thus, Galactomannan assay is a
useful diagnostic for early detection of IA in high risk patients. J Microbiol Infect Dis 2018; 8(1):8-12

Keywords

Galactomannan assay, Invasive aspergillosis, Fungal culture, Fungal smear

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