Antimicrobial effects of Rosmarinus Officinalis; in-vitro study

Year 2023, Volume: 6 Issue: 3, 463 - 466, 31.12.2023

Ugur Kesıcı , Mehmet Demirci , Mehmet Güray Duman , Yahya Kaan Karatepe , Sibel Oba

https://doi.org/10.36516/jocass.1356913

Abstract

Introduction: The plant Rosmarinus officinalis (RO) is a member of the Lamiaceae family and is more commonly known as rosemary. This study's primary objective is to compare the antimicrobial effect of RO extract at different concentrations on various microorganisms.
Material and Methods: Strains of S. aureus ATCC 29213, K. pneumoniae ATCC 13883, and E. coli ATCC 25922 obtained from the national reference center were inoculated into liquid Müller Hinton broth (Oxoid, UK) and incubated at 37 oC’ for 24 hours. To assess the antimicrobial efficacy of the RO extract, dilutions of 0.0625%, 0.125%, 0.25%, 0.5%, 1%, 2%, 3%, and 4% were prepared.
Results: The MIC value for RO extract was 2% for S. aureus ATCC 29213 and K. pneumoniae ATCC 13883 and 3% for E. coli ATCC 25283. A statistically significant difference was found between the three groups, including 0.0625% and 2% of RO, in terms of the growth rates of microorganisms (p<0.001). There was no statistically significant difference between the concentrations of 3% and 4% (p=1.00).
Discussion: The antimicrobial effect potential of RO has been demonstrated in the literature and in-vito in this study. In addition, we believe it can be used as a prophylactic or as an alternative to antimicrobial agents in the topical or systemic treatment of SSIs due to its various effects, topical, oral, and systemic use, and low cost. Thus, it is anticipated that the costs of treatment will be reduced. To determine the efficacious dose and implement it in clinical practice, experimental and clinical studies are necessary.

Keywords

S. aureus ATCC 29213, K. pneumoniae ATCC 13883, E. coli ATCC 25922, Rosmarinus officinalis, surgical site infection, antimicrobial.

Ethical Statement

İnvitro çalışma olması nedeniyle Etik Kurul Onayı gerekmemektedir.

References

• 1.ErhanY, Veral A, Kara E, et al. A clinicopathologic study of a rare clinical entity mimicking breast carcinoma: idiopathic granulomatous mastitis. The Breast. 2000; 9(1): 52-6. https://doi.org/10.1054/brst.1999.0072
• 2.Imoto S, Kitaya T, Kodama T, et al. Idiopathic Granulomatous Mastitis: Case Report and Review of the Literature, Japanese Journal of Clinical On-cology. 1997; 27(4): 274-7. https://doi.org/10.1093/jjco/27.4.274
• 3.Schelfout K, Tjalma WA, Cooremans ID, et al. Observations of an idio-pathic granulomatous mastitis. Eur J Obstet Gynecol Reprod Biol. 2001;97(2):260-7. https://doi.org/10.1016/s0301-2115(00)00546
• 4.Dobinson HC, Anderson TP, Chambers ST, et al. Antimicrobial Treatment Options for Granulomatous Mastitis Caused by Corynebacterium Species. J Clin Microbiol. 2015; 53(9): 2895-9. https://doi.org/10.1128/JCM.00760-15
• 5.Gautier N, Lanolde L, Tran-Tranh D, et al. Chronic Granulomatous Masti-tis: Imaging, Pathology and Management, European Journal of Radiology. 2013; 82 (4): e165-75. https://doi.org/10.1016/j.ejrad.2012.11.010
• 6.Al-Khaffaf B, Knox F, Bundred, NJ. Idiopathic granulomatous mastitis: a 25-year experience. J Am Coll Surg. 2008; 206(2): 269-73. https://doi.org/10.1016/j.jamcollsurg.2007.07.041
• 7.ShinYD, Park SS, Song YJ, et al. Is surgical excision necessary for the treatment of Granulomatous lobular mastitis? BMC Womens Health. 2017; 17 (1): 49. https://doi.org/10.1186/s12905-017-0412-0
• 8.Bani-Hani KE, Yaghan RJ, Matalka II, et al. Idiopathic granulomatous mas-titis: Time to avoid unnecessary mastectomies. Breast J. 2004; 10 (4): 318-22. https://doi.org/10.1111/j.1075-122X.2004.21336.x
• 9.Gi̇rgi̇n S, Uslukaya O, Yılmaz E, et al. Granulomatous mastitis: A retro-spective review of 49 patients. Dicle Medical Journal. 2015; 42(1): 22-7. https://doi.org/10.5798/diclemedj.0921.2015.01.0524
• 10.Ozel L, Unal A, Unal E, et al. Granulomatous mastitis: is it an autoim-mune disease? Diagnostic and therapeutic dilemmas. Surg Today. 2012; 42(8): 729-33. https://doi.org/10.1007/s00595-011-0046-z
• 11.Li J. Diagnosis and Treatment of 75 Patients with Idiopathic Lobular Granulomatous Mastitis. J Invest Surg. 2019; 32(5): 414-20. https://doi.org/10.1080/08941939.2018.1424270
• 12.Ozşen M, Tolunay Ş, Gökgöz MŞ. Granulomatous Lobular Mastitis: Clinicopathologic Presentation of 90 Cases. Recurrence in Uterine Tumors with Ovarian Sex-Cord Tumor Resemblance: A Case Report and Systemat-ic Review. Türk Patoloji Derg. 2018; 34(3): 215-9. https://doi.org/10.5146/tjpath.2018.01431
• 13.Agarwal C, Singh K, Pujani M, et al. Are all Granulomatous Mastitis Cases Tuberculous?: A Study on the Role of Cytology in Evaluation of Granulom-atous Mastitis. Are all Granulomatous Mastitis Cases Tuberculous?: A Study on the Role of Cytology in Evaluation of Granulomatous Mastitis. Türk Patoloji Derg. 2019; 35(2): 128-33. https://doi.org/10.5146/tjpath.2018.01442
• 14.Chandanwale S, Naragude P, Shetty A, et al. Cytomorphological Spec-trum of Granulomatous Mastitis: A Study of 33 Cases. Eur J Breast Health. 2020; 16(2): 146-51. https://doi.org/10.5152/ejbh.2020.5185
• 15.Prasad S, Jaiprakash P, Dave A, et al. Idiopathic granulomatous mastitis: an institutional experience. Turk J Surg. 2017; 33(2): 100-3. https://doi.org/10.5152/turkjsurg.2017.3439
• 16.Wolfrum A, Kümmel S, Theuerkauf I, et al. Granulomatous Mastitis: A Therapeutic and Diagnostic Challenge. Breast Care (Basel). 2018;13(6): 413-8. https://doi.org/10.1159/000495146
• 17.Shoyele O, Vidhun R, Dodge J, et al. Cystic neutrophilic granulomatous mastitis: A clinicopathologic study of a distinct entity with supporting evidence of a role for Corynebacterium-targeted therapy. Ann Diagn Pathol. 2018; 37: 51-6. https://doi.org/10.1016/j.anndiagpath.2018.08.005
• 18.Taylor GB, Paviour SD, Musaad S, et al. A clinicopathological review of 34 cases of inflammatory breast disease showing an association between corynebacteria infection and granulomatous mastitis. Pathology. 2003; 35(2): 109-9. https://linkinghub.elsevier.com/retrieve/pii/8WYF41619LM9YKP
• 19.Renshaw AA, Derhagopian RP, Gould EW. Cystic neutrophilic granu-lomatous mastitis: an underappreciated pattern strongly associated with gram-positive bacilli. Am J Clin Pathol 2011; 136(3): 424–7. https://doi.org/10.1309/AJCP1W9JBRYOQSNZ
• 20.Kıvılcım T, Altıntoprak F, Memiş B, et al. Role of Bacteriological Agents in Idiopathic Granulomatous Mastitis: Real or Not? Eur J Breast Health. 2018; 15(1): 32-6. https://doi.org/10.5152/ejbh.2018.4249
• 21.Taylor GB, Paviour SD, Musaad S, et al. A clinicopathological review of 34 cases of inflammatory breast disease showing an association between corynebacteria infection and granulomatous mastitis. Pathology. 2003; 35(2): 109-9. https://linkinghub.elsevier.com/retrieve/pii/8WYF41619LM9YKP 22.Paviour S, Musaad S, Roberts S, et al. Corynebacterium species isolated from patients with mastitis. Clin Infect Dis. 2002; 35(11): 1434-40. https://doi.org/10.1086/344463 23.Butta H, Pasha F, Dawar R, et al. Corynebacterium Amycolatum Causing Breast Abscess: An Infecting Diphtheroid with a difference. Annals of Pa-thology and Laboratory Medicine. 2017; 4: 128-30. https://doi.org/10.21276/APALM.1456