j surg med

Journal of Surgery and Medicine

2602-2079

Selçuk BAŞAK

10.28982/josam.399562

Clinical Sciences

Klinik Tıp Bilimleri

Resistance patterns of gram negative bacteria in urinary tract infections and efficacy of empirical treatment in noncomplicated cases: Retrospective cohort study of 2180 women

Üriner sistem enfeksiyonlarında gram negatif bakterilerin direnç paternleri ve komplike olmayan hastalarda ampirik tedavinin uygunluğu: 2180 kadın hastada retrospektif kohort çalışma

Varışlı

Ayşe Nuriye

Kırıkkale Yüksek İhtisas Hospital Cetin Hazırolan

Gulşen

HACETTEPE ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, MİKROBİYOLOJİ ANABİLİM DALI Kocagül Çelikbaş

Aysel

ANKARA NUMUNE EĞİTİM VE ARAŞTIRMA HASTANESİ, MİKROBİYOLOJİ VE KLİNİK MİKROBİYOLOJİ Aksoy

Altan

Ankara Numune Research and Training Hospital, Department of Clinical Microbiology

05 01 2018

2 2 99 104 02 28 2018 04 04 2018

2017

Journal of Surgery and Medicine

Aim: Community and hospital acquired urinary tract infections (UTI) are the most common bacterial infections in all age groups. In this study, the women diagnosed UTI, which complicated and uncomplicated, were referred to outpatient clinics between 2011 and 2015 were selected. The suitability of empirical antimicrobial therapy prescribed with resistance patterns of Gram negative bacteria isolated from these patients was investigated.Methods: UTI complaining and urine culture ≥10⁵ cfu / ml of bacteria that women aged 18-65 were comprised the study group. HIS (Hospital Information System Software) was scanned for these patients and uncomplicated and complicated UTI distinction was made according to the criteria set by Infectious Diseases Society of America (IDSA, 2016). Of the 2180 patients studied, 836 were complicated and 896 were noncomplicated. Identification of Gram negative isolates in urine culture; Conventional methods and Matrix-Assisted Laser Desorption and Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS), (Biotyper, Bruker, Germany) systems were used. The antimicrobial susceptibilities of the isolated strains from 2011 to 2014 were determined according to the Clinical Laboratory Standards Institute (CLSI) and the isolated strains in 2015 were determined according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST).Results: The distribution of 2180 female patients (18 to 65 years old) was urology polyclinic (68%), family medicine, infectious disease and physical medicine and rehabilitation polyclinics (19.1%), and emergency medicine polyclinic (12.6%). E. coli (84%), K. pneumonia / oxytoca (7%) and Enterobacter spp (2.6%) were the most frequently detected agents in the uncomplicated UTIs, while complicated UTI E. coli (91%), K. pneumonia / oxytoca (5.7%) and P. aeruginosa (1%) were found to be the first, second and third in the patients. Quinolones were prescribed 18.2%, phosphomycin 16.7%, nitrofurantoin 15.6%, nitrofurantoin and phosphomycin combined 16.3%, second generation oral cephalosporins 9.6% and third generation oral cephalosporins 10.7%. Resistance to quinolones, the most commonly prescribed antibiotic, was found in 19% of E. coli, 21% of Klebsiella spp., 13% of Enterobacter spp. and 9% of Proteus spp.Conclusion: When these prescriptions were compared with the results of antimicrobial susceptibility, it was observed that 38 (14%) were incompatible with the sensitivity results. It was found that 42% of cefuroxime prescriptions, 28% of quinolone prescriptions and 17% of ceftriaxone prescriptions were incompatible with the antibiogram results.

Amaç: Toplum ve hastane kaynaklı üriner sistem enfeksiyonları (ÜSE), tüm yaş gruplarında en sık karşılaşılan bakteriyel enfeksiyonlardır. Bu çalışmada 2011-2015 yılları arasında polikliniklere ÜSE şikayetiyle başvuran hastalardan izole edilen Gram negatif bakterilerin direnç paternleri ile bu hastalara reçetelenen ampirik antimikrobiyal tedavinin uygunluğunun araştırıldı.Yöntemler: ÜSE şikayetiyle başvuran ve idrar kültüründe ≥10⁵ kob/ml bakteri üremesi olan 18-65 yaş arası kadın hastalar çalışma grubunu oluşturdu. HBYS (Hastane Bilgi Yazılım Sistemi)’nden bu hastaların dosyası taranarak Infectious Diseases Society of America (IDSA, 2016) tarafından belirlenen kriterler doğrultusunda komplike olmayan ve komplike ÜSE ayrımları yapıldı. Buna göre 2180 hastanın 836’sı komplike ÜSE tanısı alırken, 896’sının nonkomplike ÜSE tanısı aldığı tesbit edildi. İdrar kültüründe izole edilen Gram negatif izolatların identifikasyonu; konvansiyonel yöntemler ve Matrix-Assisted Laser Desorption and İonization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS), (Biotyper, Bruker, Almanya) sistemleri ile yapıldı. İzole edilen suşların antimikrobiyal duyarlılıkları 2011-2014 yılları için Clinical Laboratory Standards Institute (CLSI), 2015 yılı için ise The European Committee on Antimicrobial Susceptibility Testing (EUCAST) önerilerine göre belirlendi.Bulgular: ÜSE şikayetiyle çalışmaya alınan 18-65 yaş arası 2180 kadın hastanın poliklinik dağılımı; %68 Üroloji, %19,1 Aile Hekimliği pol., Enfeksiyon pol ve Fizik Tedavi ve Rehabilitasyon pol. ve %12,6 Acil pol. idi. Komplike olmayan ÜSE’lerde 1., 2. ve 3. sırada en çok tespit edilen etken sırasıyla E. coli (%84), K. pneumoni/oxytoca (%7) ve Enterobacter spp (%2,6) iken, komplike ÜSE’li hastalarda 1., 2. ve 3. sırada E. coli (%91), K. pneumoni/oxytoca (%5,7) ve P. aeruginosa (%1) tespit edildi. Hastalara reçete edilen antimikrobiyallere bakıldığında; kinolonların %18,2 oranında, fosfomisin %16,7, nitrofurantoin %15,6, nitrofurantoin ve fosfomisin kombine olarak %16,3 oranında, 2. kuşak oral sefalosporinlerin %9,6 ve 3. kuşak oral sefalosporinlerin ise %10,7 oranında reçetelendiği saptandı. Ayaktan tedavi edilen hastalara en sık reçete edilen antibiyotik olan kinolonlara direnç, E.coli’de %19, Klebsiella spp.’de %21, Enterobacterspp.’de %13, Proteus spp’de % 9 olarak bulundu.Sonuç: Bu reçetelerin antimikrobiyal duyarlılık sonuçları ile uyumuna bakıldığında ise, 38(%14)’inin duyarlılık sonuçları ile uyumsuz olduğu gözlendi. Sefuroksim reçetelerinin %42, kinolon reçetelerinin %28, seftriakson reçetelerinin ise %17 oranında antibiyogram sonucu ile uyumsuz olduğu saptandı.

Antimicrobial resistance Empirical therapy Urinary tract infections Escherichia coli Klebsiella spp

Antimikrobiyal direnç Üriner sistem enfeksiyonu Ampirik tedavi Escherichia coli Klebsiella spp

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