Germination and Seedling Properties of Lotus corniculatus L. Under Simulated Drought Stress

Year 2023, , 879 - 889, 25.12.2023

Ramazan Beyaz

https://doi.org/10.33462/jotaf.1226444

Abstract

Drought is an important abiotic stress factor that reduces agricultural production and yield in many crops, including forage crops, in agricultural areas around the world. Lotus corniculatus L. is the agriculturally crucial perennial legume forage crop that can tolerate moderate drought. However, studies to determine the responses of L. corniculatus to drought are limited. Therefore, this study was carried out to determine the seed germination and early seedling growth properties of L. corniculatus at different PEG6000 induced-drought treatments under in vitro conditions. In order to do this, L. corniculatus (cv. ‘AC Langille’) seeds were planted in MS (Murashige and Skoog/Gamborg) medium containing 0%, 4%, and 8% (w/v) PEG6000 for 14 days. In this study, germination percentage, mean germination time, germination rate index (speed of germination), shoot and root length, root to shoot length ratio, shoot and root fresh weight, shoot and root dry weight, shoot and root dry matter ratio, root shoot dry matter ratio, shoot and root water content and seedling vigor index parameters were measured. Our results showed that increasing drought levels resulted in an overall significant reduction in germination and seedling growth parameters except shortened mean germination time (especially, 4% PEG6000 treatment) and increased shoot and root dry matter ratio at higher (especially, 8% PEG6000 treatment) drought levels. When important growth parameters such as length, fresh and dry weight, dry matter ratio and water content, which show the development of root and shoot organs, are evaluated together, it has been determined that the root is negatively affected by drought stress at a higher rate. Based on these data, it can be concluded that the L. corniculatus will suffer a high yield loss under the drought stress at the osmotic potential (-1.03 bar) created by 8% PEG6000 treatment.

Keywords

Lotus corniculatus L., Drought stress, Germination, Seedling properties, in vitro

Simüle Edilmiş Kuraklık Stresi Altında Lotus corniculatus L.'nin Çimlenme ve Fide Özellikleri

Abstract

Kuraklık, dünyadaki tarım alanlarında yem bitkileri de dahil olmak üzere birçok üründe tarımsal üretimi ve verimi azaltan önemli bir abiyotik stres faktörüdür. Lotus corniculatus L., orta derecede kuraklığı tolere edebilen, tarımsal açıdan çok önemli bir çok yıllık baklagil yem bitkisidir. Bununla birlikte, L. corniculatus'un kuraklığa verdiği tepkileri belirlemeye yönelik çalışmalar sınırlıdır. Bu nedenle bu çalışma, L. corniculatus'un farklı PEG6000 kaynaklı kuraklık uygulamalarında in vitro koşullarda tohum çimlenmesi ve erken fide büyüme özelliklerini belirlemek amacıyla yapılmıştır. Bunu yapmak için L. corniculatus (cv. 'AC Langille') tohumları, 14 gün boyunca %0, %4 ve %8 (w/v) PEG6000 içeren MS (Murashige ve Skoog/Gamborg) ortamına ekilmiştir. Bu çalışmada çimlenme yüzdesi, ortalama çimlenme süresi, çimlenme oranı indeksi (çimlenme hızı), sürgün ve kök uzunluğu, kök sürgün uzunluğu oranı, sürgün ve kök yaş ağırlığı, sürgün ve kök kuru ağırlığı, sürgün ve kök kuru madde oranı, kök sürgün kuru madde oranı, sürgün ve kök su içeriği ve fide canlılık indeksi parametreleri ölçülmüştür. Sonuçlar, artan kuraklık seviyelerinin, kısalan ortalama çimlenme süresi (özellikle %4 PEG6000 uygulamasında) ve daha yüksek sürgün ve kök kuru madde oranı (özellikle, %8 PEG6000 uygulamasında) dışında çimlenme ve fide büyüme parametrelerinde genel olarak önemli bir azalmaya yol açtığını göstermiştir. Kök ve sürgün organlarının gelişimini gösteren uzunluk, yaş ve kuru ağırlık, kuru madde oranı ve su içeriği gibi önemli büyüme parametreleri birlikte değerlendirildiğinde, kökün kuraklık stresinden daha yüksek oranda olumsuz etkilendiği tespit edilmiştir. Bu verilere dayanarak, L. corniculatus'un %8 PEG6000 uygulaması ile oluşturulan ozmotik potansiyeldeki (-1.03 bar) kuraklık stresi altında yüksek verim kaybına uğrayacağı sonucuna varılabilir.

Keywords

Lotus corniculatus L., Kuraklık stresi, Çimlenme, Fide özellikleri, in vitro

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