The Effects of Silver Nitrate and Pre-Cold Treatments on Callus Formation in Strawberry Anther Culture

Year 2024, Volume: 21 Issue: 1, 15 - 23, 30.01.2024

Sevinç Şener Ayşe Gül Nasırcılar Ahmet Karaçan

https://doi.org/10.33462/jotaf.1217960

Abstract

Strawberry (Fragaria x ananassa Duch.) which is one of the widely grown berry species in the world has economic and commercial importance. In commercial strawberry varieties, in order to increase yield and quality, it is necessary to obtain starting materials that are resistant/tolerant to biotic and abiotic stress factors. Biotechnological methods have an important place in strawberry breeding studies due to the long and costly process of classical breeding methods, the genetic expansion of seed production, high ploidy level and strong heterozygosity. Haploid plant production is an efficient breeding method that has been successfully applied to most plant species. However, due to the lack of sufficient haploid studies on strawberry and the fact that a specific protocol for this species has not yet been developed the necessary progress has not been made in this regard. In this study, the effectiveness of some factors determine the success in anther culture which has a significant place in obtaining haploid strawberries was investigated. For this reason, first, different sodium hypochlorite doses (NaOCl; 1%, 2%, 3%) and application durations (10, 15, 20 min) were used to determine the appropriate method for sterilisation, then cold pre-treatments (24, 36, 48, 72 hours at +4 °C) and different silver nitrate doses (AgNO3; 10, 20, 30, 40 mg l-1) were employed for callus induction in Festival strawberry variety. At the conclusion of the study it was observed that the lowest contamination rate (1%) was obtained by soaking in 1% sodium hypochlorite solution for 10 minutes. Cold pre-treatment of flower buds at +4 °C for 36 hours produced the highest callus induction rate (96%). The evaluation of the effect of AgNO3 application at different doses on the callus induction rate revealed that the highest callus induction (82%) was obtained from 20 mg l-1 AgNO3 dosage. This study showed that anther culture practices in strawberry can be improved by using cold pre-treatment, appropriate sterilization method and silver nitrate addition to the medium.

Keywords

Anther, Callogenesis, Silver nitrate, Micropropagation, Haploid

Gümüş Nitrat ve Ön Soğuk Uygulamalarının Çilek Anter Kültüründe Kallus Oluşumuna Etkileri

Abstract

Çilek (Fragaria x ananassa Duch.), dünyada yaygın olarak yetiştiriciliği yapılan, ekonomik ve ticari öneme sahip olan önemli üzümsü meyve türlerinden birisidir. Ticari çilek çeşitlerinde, verim ve kalitenin artırılması için biotik ve abiotik stress faktörlerine karşı dayanıklı veya tolerant başlangıç materyallerinin elde edilmesi gerekmektedir. Çilekte, klasik ıslah çalışmaları süreçlerinin uzun ve masraflı olması, tohumdan üretiminin genetik açılım göstermesi, yüksek ploidi seviyesi ve güçlü heterozigotluk sebebiyle, biyoteknolojik yöntemler ıslah çalışmalarında önemli bir yer tutmaktadır. Haploid bitki üretimi çoğu bitki türünde başarı ile uygulanan etkili bir ıslah yöntemidir. Ancak çilekte haploid bitki üretimi ile ilgili yeterli sayıda çalışmanın olmaması ve bu türe özgü bir protokolün hala geliştirilememesi gibi nedenlerle bu konuda gerekli ilerlemeler sağlanamamıştır. Bu çalışmada, haploid çilek eldesinde önemli bir yer tutan anter kültüründe başarıyı etkileyen bazı faktörlerin etkinliğinin araştırılması amaçlanmıştır. Bu nedenle, Festival çilek çeşidinde kallus indüksiyonu için öncelikle sterilizasyon için uygun yöntemi belirlemek amacıyla farklı sodyum hipoklorit dozları (NaOCl; %1, %2, %3) ve uygulama süreleri (10, 15, 20 dk) ardından farklı soğuk ön uygulamaları (+4 oC'de 24, 36, 48, 72 saat) ve gümüş nitrat dozları (AgNO3; 10, 20, 30, 40 mg l-1) kullanılmıştır. Araştırmadan elde edilen verilerin değerlendirilmesi sonucunda en düşük kontaminasyon oranının (%1) %1'lik sodyum hipoklorit çözeltisinde 10 dakika bekletilerek elde edildiği belirlenmiştir. Çiçek tomurcuklarının 36 saat boyunca +4 oC 'de soğuk ön muameleye tabi tutulması ile en yüksek kallus indüksiyon oranı (%96) sağlanmıştır. Farklı dozlarda AgNO3 uygulamasının kallus indüksiyonu üzerine etkisinin değerlendirilmesi ise en yüksek oranın (%82) 20 mg l-1 AgNO3 dozundan elde edildiğini göstermiştir. Bu çalışma, çilekte anter kültürü çalışmalarının soğuk ön uygulaması, uygun sterilizasyon yöntemi ve besi ortamına gümüş nitrat ilavesi ile geliştirilebileceğini göstermiştir.

Keywords

Anter, Kallogenesis, Gümüş nitrat, Mikroçoğaltım, Haploid

References

• Aaby, K., Grimsbo, I. H., Hovda, M. B. and Rode, T. M. (2018). Effect of high pressure and thermal processing on shelf life and quality of strawberry purée and juice. Food Chemistry, 260: 115-123.
• Andrianjaka-Camps, Z. N., Heritier, J., Ancay, A., Andlauer, W. and Carlen, C. (2017). Evolution of the taste-related and bioactive compound profiles of the external and internal tissues of strawberry fruits (Fragaria x ananassa) cv. ‘Clery’during ripening. Journal of Berry Research, 7(1): 11-22.
• Anonymous (2021). Food and Agriculture Organization of the United Nations. FAOSTAT. http://faostat.fao.org/ (Accessed date: 01.10.2021)
• Bayram, S. E., Elmacı, Ö. and Miran, B. (2016). An evaluation of strawberry production in terms of plant nutrition and farmer applications: Evidences from Gediz River Basin, Turkey. Journal of Tekirdag Agricultural Faculty, 13(1): 66-79.
• Chandler, C. K., Folta, K., Dale, A., Whitaker, V. M. and Herrington, M. (2012). Strawberry. In Fruit Breeding (Ed. M.L Badenes., D. H.). Springer, Boston.
• Daud, N. H., Jayaraman, S. and Mohamed, R. (2012). Methods Paper: An improved surface sterilization technique for introducing leaf, nodal and seed explants of Aquilaria malaccensis from field sources into tissue culture. Asia-Pacific Journal of Molecular Biology and Biotechnology, 20: 55-58.
• Davis, T. M., Denoyes-Rothan, B. and Lerceteau-Köhler, E. (2007). Strawberry. In Fruits and Nuts (Ed. C. Kole), Chapter 8:189-205. Springer, Berlin, Heidelberg.
• Dunwell, J. M. (2010). Haploids in flowering plants: origins and exploitation. Plant Biotechnology Journal, 8(4): 377-424.
• Hassan, M. F. and Islam, S. S. (2021). Effect of silver nitrate and growth regulators to enhance anther culture response in wheat (Triticum aestivum L.). Heliyon, 7(5): e07075.
• Hummer, K. E., Bassil, N. V., Zurn, J. D. and Amyotte, B. (2022). Phenotypic characterization of a strawberry (Fragaria× ananassa Duchesne ex Rosier) diversity collection. Plants, People, Planet, 5(2): 209-224.
• Irikova, T., Grozeva, S. and Rodeva, V. (2011). Anther culture in pepper (Capsicum annuum L.) in vitro. Acta Physiologiae Plantarum, 33(5): 1559-1570.
• Kim, H. J., Lee, J. N., Choi, M. J. and Suh, J. T. (2019). Comparison of in vitro propagation and occurrence of morphological and genetic variation in strawberry tissue culture with various plant hormone treatments. Journal of Plant Biotechnology, 46(2): 106-113.
• Kim, J., Lee, C. G. and Na, H. (2020). Optimal culture environment for anther-derived callus, embryo, and regeneration of strawberry ‘Jukhyang’. Horticulture, Environment, and Biotechnology, 61(6): 1031-1038.
• Kiviharju, E. and Pehu, E. (1998). The effect of cold and heat pre-treatments on anther culture response of Avena sativa and A. sterilis. Plant Cell, Tissue and Organ Culture, 54(2): 97-104.
• Lahiri, S., Smith, H. A., Gireesh, M., Kaur, G., and Montemayor, J. D. (2022). Arthropod pest management in strawberry. Insects, 13(5): 475.
• Mezzetti, B. (2013). Breeding and biotechnology for improving the nutritional quality of strawberry. Journal of Berry Research, 3(3): 127-133.
• Michalska, A., Carlen, C., Heritier, J. and Andlauer, W. (2017). Profiles of bioactive compounds in fruits and leaves of strawberry cultivars. Journal of Berry Research, 7(2): 71-84.
• Na H., Kim, B. S. and Kim J. (2019). Anther-derived callus induction based on culture medium, myo-inositol, AgNO3 and Fe-EDTA in ‘Seolhyang’ strawberries. Journal of Plant Breeding and Crop Science, 11(1): 26-32.
• Na, H. Y., Kim, D. Y. and Chun, C. H. (2011). Effects of cold pretreatment and medium composition on anther culture initiation in strawberry. Horticultural Science & Technology, 29(5): 488-493.
• Nguyen, T. X., YeSu, S. and Sungmin, P. (2012). Haploid plant production through anther culture in day-neutral strawberry (Fragaria × ananassa Duch) cv. Albion. Journal of International Society for Southeast Asian Agricultural Sciences, 18: 173-184.
• Niazian, M., Noori, S. A. S., Galuszka, P., Tohidfar, M., Mortazavian, S. M. M. (2017). Genetic stability of regenerated plants via indirect somatic embryogenesis and indirect shoot regeneration of Carum copticum L. Industrial Crops and Products, 97: 330-337.
• Pehlivan, E. C., Kunter, B. and Royandazagh, S. D. (2017). Choise of explant material and media for in vitro callus regeneration in Sultana grape cultivar (Vitis vinifera L.). Journal of Tekirdag Agricultural Faculty, The special Issue of 2nd International Balkan Agriculture Congress, 30-34.
• Shahvali-Kohshour, R., Moieni, A. and Baghizadeh, A. (2013). Positive effects of cold pre-treatment, iron source, and silver nitrate on anther culture of strawberry (Fragaria× ananassa Duch.). Plant Biotechnology Reports, 7(4): 481-488.
• Shariatpanahi, M. E., Bal, U., Heberle Bors, E. and Touraev, A. (2006). Stresses applied for the reprogramming of plant microspores towards in vitro embryogenesis. Physiologia Plantarum, 127(4): 519-534.
• Simpson, D. (2018). The Economic Importance of Strawberry Crops. In the Genomes of Rosaceous Berries and Their Wild Relatives (Ed. T. Hytönen, J., Graham, R., Harrison). Springer, Cham.
• Tyagi, V. S. A., Chauhan, P., Kumari, P. and Kaushal, S. (2011). Identification and prevention of bacterial contamination on explant used in plant tissue culture labs. International Journal of Pharmacy and Pharmaceutical Sciences, 3(4): 160-163.
• Witter, S., Radin, B., Lisboa, B. B., Teixeira, J. S. G., Blochtein, B. and Imperatriz-Fonseca, V. L. (2012). Performance of strawberry cultivars subjected to different types of pollination in a greenhouse. Pesquisa Agropecuária Brasileira, 47: 58-65.