Preparation and Determination of In Vivo and In Vitro Performance of Doxycycline Imprinted Contact Lenses for Corneal Neovascularization Treatment

Year 2018, Volume: 5 Issue: 3, 1185 - 1192, 01.09.2018

Sibel Erkal Ilhan Özge Kürkçüoğlu , Tuğçe Inan Özde Zeynep Güner Dilek Dalgakıran Begüm Okutan Gamze Torun Köse , Ayça Kırmızı Aylin Okçu Heper Özlem Gürses F. Seniha Güner

https://doi.org/10.18596/jotcsa.428846

Cited By: 1

Abstract

The aim of this study is to develop doxycycline
imprinted contact lenses that will be used in the treatment of corneal
neovascularization, which can eventually cause blindness. For this purpose,
doxycycline imprinted contact lenses were first prepared, and then they were
loaded with doxycycline and their in
vitro and in vivo performances
were determined. In the synthesis of the contact lenses, 2-hydroxyethyl
methacrylate was used as a backbone monomer. The functional monomer was
selected as itaconic acid using molecular simulations. Doxycycline release
profile of the lenses was determined in NaCl solution and their cytotoxic
response was investigated on retinal pigment epithelium cells. In vivo experiments in rat models were
performed to study the treatment patterns. The rats were sacrificed fifteen
days after treatment, and clinical examination under optical microscope was
performed to evaluate neovascularization, infiltration of inflammatory cells,
and corneal epithelial changes. In conclusion; doxycycline imprinted contact
lenses promise as an effective treatment method for corneal neovascularization.

Keywords

Corneal neovascularization, Contact lens, Doxycycline

References

• 1. Aquil M, Gupta H. Contact lenses in ocular therapeutics. Drug Discovery Today. 2012;17(9-10):522-7.
• 2. Forbes JM, Cooper ME. Mechanisms of diabetic complications. Physiological Reviews. 2013;93:137-88.
• 3. Yoeruek E, Ziemssen F, Henke-Fahle S, Tatar O, Tura A, Grisanti S, Bartz-Schmidt KU, Szurman P. Safety, penetration and efficacy of topically applied bevacizumab: evaluation of eyedrops in corneal neovascularization after chemical burn. Acta Ophthalmologica, 2008;86:322-8.
• 4. Giulio F, Dastjerdi MH, Okanobo A, Cheng SF, Amparo F, Nallasamy N, Dana R. Topical ranibizumab as a treatment of corneal neovascularization. Cornea. 2013;32(7):992-7.
• 5. Feizi S, Azari AA, Safapour S. Therapeutic approaches for corneal neovascularization. Eye and Vision. 2017;4:28-38.
• 6. Byrne ME, Salian V. Molecular imprinting within hydrogels II: Progress and analysis of the field. International Journal of Pharmaceutics. 2008;364:188-212.
• 7. Cheong WJ, Yang SH, Ali F. Molecular imprinted polymers for separation science: A review of reviews. Journal of Separation Science. 2013;36(3):60928.
• 8. Magdy Elnashar, editor. Biopolymers. IntechOpen; 2010. Open access peer-reviewed chapter 28. Molecularly Imprinted Polymers (PIMs) in Biomedical Applications by Puoci F, Cirillo G, Curcio M, Iemma F, Parisi OI, Spizzirri UG, Picci N. ISBN: 978-953-307-109-1.
• 9. Hu X, Hao, L, Wang, H, Yang X, Zhang G, Wang G, Zhang X. Hydrogel contact lens for extended delivery of ophthalmic drugs. International Journal of Polymer Science. 2011; Article ID 814163. 10. Alverez-Lorenzo C, Yanez F, Barreiro-Iglesias R, Concheiro A. Imprinted soft contact lenses as norfloxacin delivery systems. Journal of Controlled Release. 2006;113(3)236–44.
• 11. Garcia DM, Escobar J., Noa Y, Bada N, Hernaez E, Katime I. Timolol maleate release from pH-sensible poly(2-hydroxyethyl methacrylate-co-methacrylic acid) Hydrogels. European Polymer Journal. 2004;40(8):1683-90.
• 12. Tieppo A, White CJ, Paine AC, Voyles ML, McBride MK, Byrne ME. Sustained in vivo release from imprinted therapeutic contact lenses. Journal of Controlled Release. 2012;157(3):391-7.
• 13. Vasapollo G, Sole RD, Mergola L, Lazzoi MR, Scardino A, Scorrano S, Mele G. Molecularly Imprinted Polymers: Present and Future Prospective. International Journal of Molecular Science. 2011; 12:5908-45.
• 14. Dalgakiran D, Inan T, Güner FS. Investigation of photoinduced polymerization of doxycycline-imprinted hydrogels: effect of template on initiator reactivity, conversion, and reaction rate. Turkish Journal of Chemistry. 2017;41: 862-73.
• 15. Gordon MK, DeSantis A, Deshmukh M, Lacey CJ, Hahn RA, Beloni J, Anumolu SS, Schlager JJ, Gallo MA, Gerecke DR, Heindel ND, Svoboda KKH, Babin MC, Sinko PJ. Doxycycline Hydrogels as a Potential Therapy for Ocular Vesicant Injury. Journal of Ocular Pharmacology and Therapeutics. 2010;26(5):407-19.
• 16. Lobo MS, Costa P. Modeling and Comparison of Dissolution Profiles. European Journal of Pharmaceutical Sciences. 2001;13:123-33.