Pentafluoropropionic Anhydride Functionalized PAMAM Dendrimer as miRNA Delivery Reagent

Year 2018, Volume: 5 Issue: 3, 1295 - 1302, 01.09.2018

Ali Oztuna , Hasan Nazir

https://doi.org/10.18596/jotcsa.463855

Cited By: 4

Abstract

Poly(amidoamine) (PAMAM) dendrimers are good
candidates for nucleic acid delivery with their well-defined characteristics.
MicroRNA (miRNA) mediated regulation of biological process are also active area
of investigation. Fibroblast cells, such as MRC-5, are one of the cell lines
used in biological researches due to their hard to transfect nature. In this two-staged
study, cystamine core G5 PAMAM dendrimers were synthesized and modified with pentafluoropropionic
anhydride (PA) and subsequently tested as miRNA delivery reagent on MRC-5
cells. Effect of fluorination against to naked G5 dendrimer over transfection
efficiency was also investigated by molecular docking and quantitative
structure-activity relationship (QSAR) calculations. Structural
characterization of the synthesized dendrimers was verified by spectroscopic
techniques. Gel retardation assay, particle size and transmission electron
microscopy results demonstrated polyplex formation ability of fluorinated
dendrimers with miRNA at nanoscale level. Zeta potential values indicated
non-aggregation and increased stability of the polyplexes. Prepared polyplexes
with fluorinated dendrimer showed over 90% cell viability and transfection
efficiency. In silico calculations confirmed the stable complexation with miRNA
and smooth penetration capability into the cell.

Keywords

Cystamine-G5-PAMAM, pentafluoropropionic anhydride, miRNA, MRC-5

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