Can soybean lipoxygenases be real models for mammalian lipoxygenases? A bioinformatics approach

Year 2021, Volume: 8 Issue: 1, 79 - 102, 28.02.2021

Sevilay Cengiz Levent Cavas

https://doi.org/10.18596/jotcsa.785109

Abstract

Soybean lipoxygenases (soybean lipoxygenase-1) has been a well-studied enzyme because it is considered as a model enzyme for mammalian lipoxygenases. In general, the soybean lipoxygenase-1 is used in the test of inhibitory activities of various compounds. The present study provides a bioinformatics approach for comparison of various lipoxygenases in the databases. Their various physical and chemical parameters such as molecular weight, theoretical pI, amino acid composition, aliphatic index and grand average of hydropathicity and the multiple sequence alignments of the lipoxygenases were computed by using several bioinformatics tools. In order to see phylogenetic relation among lipoxygenases, a phylogenetic tree was constructed. The first three most abundant amino acids in soybean lipoxygenase-1 and 15-lipoxygenase (human) are L (10.3 %), S (7.4 %), A (6.7 %) and L (13.3 %), G (7.4 %), V (7.1 %), respectively. According to the phylogenetic tree, the soybean lipoxygenases are within separate clade compared to the mammalian lipoxygenases. In conclusion, soybean lipoxygenase-1 may not fully characterise the human lipoxygenase-15 since there are remarkable sequence-based differences, which are obtained by using bioinformatics tools between soybean lipoxygenase-1 and lipoxygenases from other sources especially from human. In this context, the researchers aware of the problems described above and having similar concerns have begun to use recombinant DNA technology to produce recombinant h-15-LOX enzyme in Escherichia coli.

Keywords

bioinformatics, lipoxygenases, soybean lipoxygenase

Thanks

We thank Prof. Dr. Theodore Holman from Chemistry and Biochemistry Department of UC Santa Cruz for sharing his valuable comments about LOXs.

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