Antimalarial Evaluation of the Chemical Constituents Isolated from Dendrocalamus asper

Abstract

Bamboo shoots of Dendrocalamus asper is very important for human health because of its high content of dietary fiber, low sugar, negligible amount of fat, rich in vitamins and minerals. In search of new potential antimalarial agents, the chemical constituents of bamboo shoots were evaluated againts the P. falciparum strain 3D7. One new metabolite, (11E,13E,17E,19E)-dimethyl-15,16-dibutoxytriconta-11,13,17,19-tetraenedioate (1) along with the four known compounds; β-sitosterol (2), methyl-4-hydroxybenzoate (3), 1-methoxy-4-(methoxymethyl)benzene (4) and 4-hydroxybenzaldehyde (5) were isolated from the crude extract using chromatographic methods: MPLC, UPLC/MS, analytical and preparative HPLC. Among these, compounds 1, 3 and 4 showed promising antimalarial activity with IC50 between 0.8-2.2 μg/mL. The molecular docking between the most potent compound 3 and dihydrofolate reductase-thymidylate synthase (DHFR-TS) was done to understand and exploring ligand-receptor interactions and hypothesize the compound's refinements

Keywords

• Dendrocalamus asper
• antimalarial
• P. Falciparum
• Molecular docking
• dihydrofolate reductase-thymidylate synthase (DHFR-TS)

Supporting Institution

Universiti Sains Malaysia

Project Number

USM RUT grant (1001/PKIMIA/8011072)

Thanks

Wong, K.T also extended his appreciation to Prof Osada, H., Nogawa, T., and Futamura, Y. from Chemical Biology Research Group, RIKEN Center for Suitainable Resource Science, Wako, Saitama, Japan for providing laboratory facilities and technical assistance under short-term International Program Associate (IPA). We also thanked to Zhang J.L for his contribution for this project.

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