j. res. pharm. Journal of Research in Pharmacy 2630-6344 Marmara University 10.29228/jrp.873 Basic Pharmacology Temel Farmakoloji Hinokitiol, a natural tropolone derivative attenuates inflammoalgesia induced by LPS in experimental animals https://orcid.org/0000-0001-7822-2859 Gunjegoankar Shivshankar Department of Pharmacology, Faculty of ASPM's KT Patil College of Pharmacy https://orcid.org/0000-0002-9098-9283 Bhaleroa Aparna Department of Pharmaceutics, Faculty of JSPM’s Charak College of Pharmacy and Research https://orcid.org/0000-0002-4069-4866 Joshi Amol Department of Pharmacognosy, Faculty of ASPM's KT Patil College of Pharmacy https://orcid.org/0009-0003-7099-2404 Dherange Kishor Department of Pharmaceutics, Faculty of JSPM’s Charak College of Pharmacy and Research https://orcid.org/0009-0008-0776-4771 Pimparkar Samarth Department of Pharmaceutics, Faculty of JSPM’s Charak College of Pharmacy and Research https://orcid.org/0000-0001-8023-0145 Sawant Amruta Department of Pharmacology, Faculty of ASPM's KT Patil College of Pharmacy, Siddharth Nagar 06 28 2025 28 6 1989 2000 07 13 2023 02 12 2024 Copyright © 2010, Journal of Research in Pharmacy 2010 Journal of Research in Pharmacy

Hinokitiol is a natural bioactive compound synthesized as a secondary metabolite in numerous aromatic, medicinal plants and commonly used in food and cosmetic industries. The investigation was carried out to screen the potential activity of hinokitiol against LPS-induced inflammation, algesia, and oxidative stress in experimental animals. The hinokitiol was screened in two doses, i.e. 0.2 mg/kg (H-1), 0.4 mg/kg (H-2), and inflammoalgesia were induced by intraplantar administration of lipopolysaccharides (LPS) at a dose of 1 mg/kg. The assessment was carried out on the 7th, 14th, 21st, and 28th days for the severity of arthritic hyperalgesia score, secondary inflammatory lesions, stair climbing ability, motility, and dorsal flexion-pain score test. Serum analysis was carried out for tumor necrosis factor-alpha (TNF α), interleukin (IL-2 and IL-6), prostaglandin (PGE-2), nitric oxide (NO), and thymus/spleen index. The joint samples were further subjected to histopathological observations. The results showed that treatment with H-1 and H-2 shows dose-dependent significant decreases (P<0.05) in arthritic hyperalgesia and secondary lesions, whereas a significant increase (P<0.05) in stair climbing ability, motility, and dorsal flexion pain score test was noted. Treatment with H-1 and H-2 shows significant lowering (P<0.05) in serum TNF-α, IL-2, IL-6, PGE-2, NO, and thymus/spleen index. Histopathological observations indicated minimal damage and restored the synovial structure. The present study reveals that hinokitiol shows significant anti-inflammatory, anti-algesic, and antioxidant effects against LPS-induced inflammation in experimental animals.

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