Viola odorata, or sweet violet, is a plant rich in phytochemicals like flavonoids, tannins, saponins, and cyclotides, known for their antioxidant and antimicrobial properties, making it a promising candidate for anti-ulcer therapy. We aimed to evaluate the anti-ulcer potential of a methanolic extract of Viola odorata (MEVO) using in-vitro and in-vivo ethanol-induced gastric ulcer in rats. Fordtran’s and Vatier’s model were used as an in-vitro model which showed that MEVO at 250 mg/ml and 500 mg/ml effectively neutralized acid. The zones of inhibition of MEVO at 250 mg/ml and 500 mg/ml were found to be 9.33 mm and 11.66 mm respectively, compared to amoxicillin (14.33 mm). In the ethanol-induced ulcers rat model, the ulcer index of MEVO (500 mg/ml) was found to be 3.66, while the standard group had an ulcer index of 2.83. These results suggest that V. odorata extract possesses acid-neutralizing and antibacterial properties in both in-vitro and in-vivo settings, indicating its potential for anti-ulcer therapy.