j. res. pharm. Journal of Research in Pharmacy 2630-6344 Marmara University 10.12991/jrespharm.1634614 Pharmacology and Pharmaceutical Sciences (Other) Eczacılık ve İlaç Bilimleri (Diğer) Study on ethnopharmacy of medicinal plants for antidiarrheal purposes among the Osing Tribe using Near Infrared Spectroscopy and chemometrics techniques Cendekiawan Khrisna Agung Division of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Sanata Dharma University, Campus 3 Paingan Maguwoharjo Depok Sleman, Susanti Dhina Ayu Pharmacy Study Program, Universitas dr. Soebandi, Wijiani Nina Faculty of Pharmacy, Jember University, Jalan Kalimantan No 37 Jember Octa Riswanto Florentinus Dika Division of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Sanata Dharma University, Campus 3 Paingan Maguwoharjo Depok Sleman 03 03 2025 29 1 222 229 01 16 2024 03 31 2024 Copyright © 2010, Journal of Research in Pharmacy 2010 Journal of Research in Pharmacy

Traditional healing culture has long been known in Indonesia, this is proven by the existence of inscriptions that tell about herbal medicine that has been used for generations. As time goes by, the use of plants as medicine has decreased. This is because the inheritance process is only verbal and there is no documentation about these medicinal plants. This encourages research and preservation of plants as medicine, one of which is ethnopharmacy. The implementation of ethnopharmacy in the Osing tribe regarding the use of medicinal plants as anti-diarrhea can provide knowledge about the existence of plants that contain flavonoid compounds which have the potential to act as anti diarrhea. This study aimed at verifying the anti-diarrheal attributes of medicinal plant compounds, Near Infra-Red (NIR) spectroscopy and chemometric techniques were employed. Leveraging the established principle of flavonoid compounds as potent antioxidants capable of stabilizing the digestive tract, we employed flavonoid standards as a reference benchmark for assessing the anti-diarrheal efficacy of these compounds. This approach facilitated a comprehensive evaluation to verify the extent of their anti-diarrheal potential. The results of this research found that the guava leaf plant is widely used by the Osing tribe as an anti-diarrhea. Next, guava leaves were analyzed using Near Infra Red (NIR) spectroscopy and chemometric techniques, and test results were obtained in the form of guava leaf samples from the Osing tribe that contain flavonoids so they can be used for antidiarrheal therapy.

 Ethnopharmacy antidiarrhea spectroscopy chemometrics [1] Reyes-García V. The Relevance of Traditional Knowledge Systems for Ethnopharmacological Research: Theoreticaland Methodological Contributions. J Ethnobiol Ethnomed. 2010; 6: 1-12. https://doi:10.1186/1746-4269-6 32/METRICS. [2] Prasetyo B, Chikmawati T, Walujo E.B, Amzu E. Ethnoecology: The traditional landscape of Osing Tribe in Banyuwangi, Indonesia. Biodiversitas. 2018; 19(6): 2003–2009. https://doi:10.13057/BIODIV/D190604. [3] Kusumo DW, Sulistiyowati EL, Rohmah H, Melinda NA. Ethno-pharmaceutical study of medicinal plants for care and treatment of wounds in Indonesia: Systematic data search and preclinical review. J Jamu Indones. 2023; 8: 1–9. https://doi:10.29244/JJI.V8I1.232. [4] Sumarni W, Sudarmin S, Sumarti SS. The scientification of Jamu: A study of Indonesian’s traditional medicine. J Phys Conf Ser. 2019; 1321(3): 1–7. https://10.1088/1742-6596/1321/3/032057. [5] Bhagawan WS, Suproborini A, Prastya Putri DL, Nurfatma A, Putra RT. Ethnomedicinal study, phytochemical characterization, and pharmacological confirmation of selected medicinal plant on the northern slope of mount Wilis, East Java, Indonesia. Biodiversitas. 2022; 23(8): 4303–4313. https://doi:10.13057/BIODIV/D230855. [6] Puspitasari E, Damayanti AE, Zikrina INS, Dianasari D. Exploring the ethnopharmaceutical plants of Osing Tribe in Banyuwangi Regency: Potential application for COVID-19 Therapy. Ulum Islam. 2021; 33: 113–120. https://doi.org/10.33102/uij.vol33no3.330. [7] Fakayode SO, Baker GA, Bwambok DK, Bhawawet N, Elzey B, Siraj N, Macchi S, Pollard DA, Perez RL, Duncan AV. Molecular (Raman, NIR, and FTIR) Spectroscopy and multivariate analysis in consumable products analysis. Appl Spectros Rev. 2020; 55(8): 647–723. https://doi:10.1080/05704928.2019.1631176. [8] Hair JF, Hult GTM, Ringle CM, Sarstedt M, Danks NP, Ray S. Partial Least Squares Structural Equation Modeling (PLS-SEM) Using R. Springer. 2021: 197 https://10.1007/978-3-030-80519-7. [9] Irnawati, Riyanto S, Martono S, Rohman A. Analysis of palm oil as oil adulterant in olive and pumpkin seed oils in ternary mixture systems using FTIR Spectroscopy and Chemometrics. Int J Appl Pharm. 2019; 11(5): 210–215. https://doi:10.22159/ijap.2019v11i5.34274. [10] Windarsih A, Rohman A, Irnawati, Riyanto S. The combination of vibrational spectroscopy and chemometrics for analysis of milk products adulteration. Int J Food Sci. 2021; 2021: 8853358. https://doi:10.1155/2021/8853358. [11] Gad HA, El-Ahmady SH, Abou-Shoer MI, Al-Azizi MM. Application of chemometrics in authentication of herbal medicines: A review. Phytochem Anal. 2013; 24: 1–24. https://doi:10.1002/pca.2378. [12] Farag RS, Abdel-Latif MS, Abd El Baky HH, Tawfeek LS. Phytochemical screening and antioxidant activity of some medicinal plants’ crude juices. Biotechnol Rep. 2020; 28: e00536. https://doi:10.1016/J.BTRE.2020.E00536. [13] Luo D, Shao Y. Classification of Chinese herbal medicine based on ımproved LDA algorithm using machine olfaction. Appl Mech Mater. 2013; 239–240:1532–1536. https://doi:10.4028/www.scientific.net/AMM.239-240.1532. [14] Singh YD, Panda MK, Satapathy KB. Ethnomedicine for drug discovery. Adv Pharm Biotechnol Recent Prog Futur Appl. 2020; 15–28. https://doi:10.1007/978-981-15-2195-9_2/COVER. [15] Suryana S, Junaedi EC, Choerunisa Y, Prasetiawati R, Lubis N. Analysis of flavonoid in extract of rose Guava (Syzigium Jambos (L.) Alston) leaves using Infrared Spectroscopy and chemometrics. J Phys Conf Ser. 2019; 1402(5): 1–4. https://doi:10.1088/1742-6596/1402/5/055044. [16] Laily N, Kusumaningtyas RW, Sukarti I, Rini MRDK. The potency of Guava psidium Guajava (L.) leaves as a functional ımmunostimulatory ıngredient. Procedia Chem. 2015; 14: 301–307. https://doi:10.1016/J.PROCHE.2015.03.042. [17] Zaman W, Ye J, Saqib S, Liu Y, Shan Z, Hao D, Chen Z, Xiao P. Predicting potential medicinal plants with phylogenetic topology: Inspiration from the research of traditional Chinese Medicine. J Ethnopharmacol. 2021; 281: 114515. https://doi:10.1016/J.JEP.2021.114515. [18] Rinnan Å, van den Berg F, Engelsen SB. Review of the most common pre-processing techniques for Near-Infrared Spectra. TrAC - Trends Anal Chem. 2009; 28: 1201–1222. https://doi:10.1016/j.trac.2009.07.007. [19] Bevilacqua M, Bro R, Marini F, Rinnan Å, Rasmussen MA, Skov T. Recent chemometrics advances for foodomics. TrAC - Trends Anal Chem. 2017; 96: 42–51. https://doi:10.1016/J.TRAC.2017.08.011. [20] Brereton RG, Jansen J, Lopes J, Marini F, Pomerantsev A, Rodionova O, Roger JM, Walczak B, Tauler R. Chemometrics in analytical chemistry—Part II: Modeling, validation, and applications. Anal Bioanal Chem. 2018; 410: 6691–6704. https://doi:10.1007/s00216-018-1283-4. [21] Sima IA, Andrási M, Sârbu C. Chemometric assessment of chromatographic methods for herbal medicines authentication and fingerprinting. J Chromatogr Sci. 2018; 56: 49–55. https://doi:10.1093/chromsci/bmx080. [22] Nurani LH, Rohman A, Windarsih A, Guntarti A, Riswanto FDO, Lukitaningsih E, Fadzillah NA, Rafi M. Metabolite fingerprinting using 1H-NMR Spectroscopy and chemometrics for classification of three Curcuma species from different origins. Molecules. 2021; 26: 7626. https://doi.org/10.3390/molecules26247626. [23] Kumar M, Tomar M, Amarowicz R, Saurabh V, Sneha Nair M, Maheshwari C, Sasi M, Prajapati U, Hasan M, Singh S. Guava (Psidium Guajava L.) leaves: Nutritional composition, phytochemical profile, and health-promoting bioactivities. Foods. 2021; 10(4): 752. https://doi:10.3390/FOODS10040752. [24] Amat-Ur-rasool H, Symes F, Tooth D, Schaffert LN, Elmorsy E, Ahmed M, Hasnain S, Carter WG. Potential nutraceutical properties of leaves from several commonly cultivated plants. Biomolecules 2020; 10(11) : 1556. https://doi:10.3390/BIOM10111556.