j. res. pharm.

Journal of Research in Pharmacy

2630-6344

Marmara University

10.12991/jrespharm.1644406

Pharmacology and Pharmaceutical Sciences (Other)

Eczacılık ve İlaç Bilimleri (Diğer)

Effect of different additives on amylase activity

Herenda

Safija

Department of Chemistry, Faculty of Science, University of Sarajevo, Sarajevo, đorđević

Nataša

Department of Biomedical Sciences, State University of Novi Pazar Fetahović

Selma

Department of Chemistry, Faculty of Science, University of Sarajevo, Sarajevo Klisara

Tamara

Department of Biology, Faculty of Science, University of Sarajevo, Sarajevo Prevljak

Sabina

Clinical Center of the University of Sarajevo, Organizational unit clinic for radiology Hasković

Edhem

Department of Biology, Faculty of Science, University of Sarajevo, Sarajevo

03 03 2025

29 1 346 351 02 02 2024 03 16 2024

2010

Journal of Research in Pharmacy

Enzymes are catalysts of biological origin, and according to their chemical composition, they are simple or complex proteins. There are several theories about the enzyme's mechanism of action. Today, the Michaelis-Menten theory is generally accepted. According to this theory, during enzymatic reactions, an intermediate compound is created between the enzyme and the substrate. After the formation of this complex, the enzyme catalyzes a chemical reaction that changes the substrate into another molecule, which we call the product. The product is then separated and released from the active site of the enzyme, which is then ready to bind the next substrate molecule. Enzyme activity can be affected by different molecules. The purpose of this study is to use the spectrophotometric approach to determine whether sodium benzoate and ascorbic acid (vitamin C) serve as activators or inhibitors of enzymatic reactions. The obtained results show that both additives bind to the enzyme-substrate complex, causing non-competitive inhibition.

Enzymes additives ascorbic acid sodium benzoate inhibition

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