j. res. pharm.

Journal of Research in Pharmacy

2630-6344

Marmara University

10.12991/jrespharm.1644613

Pharmacology and Pharmaceutical Sciences (Other)

Eczacılık ve İlaç Bilimleri (Diğer)

Association of paraoxonase-1 108 C >T gene polymorphism with polycystic ovarian syndrome

Kadhum

Hiba Hayder

Department of Clinical Laboratory Science, College of Pharmacy, University of Baghdad Alizzi

Fadia Jasim

Department of Obstetrics and Gynecology, College of Medicine, Al-Mustansiriyah University Al-mayah

Qasim Sharhan

Medical Research Unit, College of Medicine, Al-Nahrain University, Al Timimi

Raid Jasim

Department of Chemistry and Biochemistry, College of Medicine, Al-Nahrain University,

03 03 2025

29 1 418 423 02 22 2024 03 11 2024

2010

Journal of Research in Pharmacy

Polycystic ovarian syndrome (PCOS) is increasingly recognized as a significant health concern among women of reproductive age, exerting its influence on the reproductive system and overall female physiology. Paraoxonase-1 (PON1) gene polymorphism, -108 C >T in the promoter region, have been identified as factors that influence both the stability of the enzyme and its active site. This, in turn, contributes to increase oxidative stress, a recognized risk factor associated with PCOS. This study aimed to investigate the connection between paraoxonase-1-108 C >T gen polymorphisms with PCOS in Iraqi women in a case-control study included 40 women with PCOS and 40 women with normal cycles and no symptoms of hyperandrogenism, and no history of PCOS. Whole blood was used to extract the DNA, and using specified sets of primers, the gene fragments corresponding to the -108 C >T were amplified using a conventional polymerase reaction (PCR). Direct sequencing was used to carry out the genotyping and the results showed that the age, body mass index (BMI), and comorbidities of the two groups were similar. The prevalence of homozygous genotype (TT) of the -108 C >T was greater in PCOS women (27.5%) compared to healthy women (10%), with a significant difference (OR= 5.04, 95%CI=1.11-22.97, p=0.037). The prevalence of the mutant allele (T) was found to be greater in women with PCOS (56.25% versus 141.25%) compared to healthy women. This difference was nearly statistically significant (OR=1.85, 95%CI= 0.98–3.43, p= 0.059). These results confirmed the significance of -108 C >T as a risk factor for PCOS in Iraqi women.

PCOS Paraoxonase-1 PON1 -108 C>T gene polymorphisms PON1 (rs705397) gene polymorphisms

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