j. res. pharm. Journal of Research in Pharmacy 2630-6344 Marmara University 10.12991/jrespharm.1666362 Pharmacology and Pharmaceutical Sciences (Other) Eczacılık ve İlaç Bilimleri (Diğer) Evaluation of platelet-derived growth factor loaded polycaprolactone/collagen core-shell nanofibers as guided tissue membrane Limoee Mazdak Nano Drug Delivery research center, Kermanshah University of Medical Sciences, Kermanshah Rasekhian Mahsa Pharmaceutical sciences research center, School of Pharmacy, Kermanshah University of Medical Sciences Moradipour Pouran School of Chemical Engineering, College of Engineering, University of Tehran Pourmanouchehri Zahra Pharmaceutical sciences research center, School of Pharmacy, Kermanshah University of Medical Sciences Behbood Leila Pharmaceutical sciences research center, Health Institute, Kermanshah University of Medical Sciences 04 08 2025 29 2 820 832 12 25 2022 06 06 2024 Copyright © 2010, Journal of Research in Pharmacy 2010 Journal of Research in Pharmacy

Guided tissue regeneration (GTR) is a dental surgical procedure that uses barrier membranes to guide the growth of bone, tissue, or gingiva in small places for proper function, beauty, or restoration of the prosthesis. Recently, many efforts have been made to accelerate tissue repair by adding agents such as growth factors to the GTR structure in a targeted and controlled manner of drug release, which makes the treatment more effective and reduces its side effects. The aim of this study was the preparation and evaluation of growth factor-loaded, biodegradable fibers, as a GTR membrane in oral cavity disease. In this study, two electrostatic systems of polycaprolactone (PCL) (shell) and plateletderived growth factor (PDGF) loaded collagen (core) were fabricated via coaxial electrospinning. Core-shell fibers were analyzed by FTIR, SEM, TEM, and ELISA techniques to determine the PDGF release and supplemented via in-vitro cytotoxicity, proliferation, and real-time PCR investigation. FT-IR shows that the fiber's constituents do not interfere with each other. The diameter of the nanofibers is in the range of 400 nm, and the results of the TEM images show that the core-shell structure is formed. PDGF is released from fibers in a controlled manner. The fibers do not show any cellular toxicity, have a positive effect on cell proliferation, and increase the number of cells.

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