j. res. pharm.

Journal of Research in Pharmacy

2630-6344

Marmara University

Pharmacology and Pharmaceutical Sciences (Other)

Eczacılık ve İlaç Bilimleri (Diğer)

Endophytic Bacillus spp. isolated from Archidendron pauciflorum: Pharmacological property and their phytochemical constituents

https://orcid.org/0000-0003-2227-5040

Priyanto

Jepri Agung

Division of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences,

https://orcid.org/0000-0003-2500-1264

Prastya

Muhammad Eka

Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research, and Innovation Agency (BRIN), Kawasan Sains dan Teknologi (KST) B.J Habibie (PUSPIPTEK) Serpong

https://orcid.org/0000-0003-1561-6943

Astuti

Rika Indri

Division of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences

https://orcid.org/0000-0002-0430-1906

Minart

Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research, and Innovation Agency (BRIN)

https://orcid.org/0000-0002-3280-9918

Mozef

Tjandrawati

Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research, and Innovation Agency (BRIN), Kawasan Sains dan Teknologi (KST) B.J Habibie (PUSPIPTEK)

06 28 2025

27 6 2511 2521 02 22 2023 04 03 2023

2010

Journal of Research in Pharmacy

Endophytic Bacillus spp. associated with Archidendron pauciflorum could be the potential source of active compounds attributed to the pharmacological property. The present study aimed to determine the antioxidant, antibacterial, antibiofilm activities, and phytochemical profiles of the crude extracts derived from four endophytic Bacillus spp. isolated from A. pauciflorum. Among all extracts tested, extract from Bacillus pseudomycoides strain DJ8 displayed the strongest antioxidant activity against DPPH and ABTS with IC50 values of 24.87±1.81 and 9.18±0.10 µg/mL, respectively. As evaluated by the dual culture-agar diffusion and disc diffusion methods, three out of four Bacillus spp. extracts also exhibited broad spectra of antibacterial activity against four ATCC strains. Among other extracts, B. pseudomycoides strain DJ8 extract became the most active extract inhibiting Staphylococcus aureus strain ATCC 6538, indicated by the lowest MIC (31.25 µg/mL) and MBC (125 µg/mL), which is considered a highly active antibacterial agent. Three extracts attributed to broad spectra antibacterial activity were also capable to inhibit biofilm formation of four ATCC strains, up to 62.59% in 2 MIC of the extract concentration. Phytochemicals (flavonoid, alkaloid, and terpenoid) were present in four endophytic Bacillus extracts. Among other extracts, an extract derived from B. pseudomycoides strain DJ8 contains the highest flavonoid content (40.10±1.18 mg QE/g extract) and phenol (58.50±4.56 mg GAE/g extract). In conclusion, these four endophytic Bacillus spp. could be the source of pharmaceutically valuable compounds attributed to antioxidant, antibacterial, and antibiofilm properties.

Antibacterial antibiofilm antioxidant Bacillus endophyte

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