j. res. pharm. Journal of Research in Pharmacy 2630-6344 Marmara University Pharmacology and Pharmaceutical Sciences (Other) Eczacılık ve İlaç Bilimleri (Diğer) Hepatoprotective effect of the ethyl acetate fraction of Indonesian Artocarpus species against CCl4 induced liver injury in rat https://orcid.org/0000-0001-9778-8866 Fitrya Fitrya Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya https://orcid.org/0000-0001-5372-8079 Amriani Annisa Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya https://orcid.org/0000-0002-3959-4681 Puspa Novita Rennie Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya https://orcid.org/0009-0000-0113-9897 Fatya Sahara Venny Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya https://orcid.org/0009-0004-3220-8313 Rizki Mella Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya https://orcid.org/0009-0004-4173-1499 Rahma Meilita Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Sriwijaya https://orcid.org/0000-0002-8332-1031 Muharni Muharni Department of Pharmacy, Faculty of Pharmacy, Universitas Andalas https://orcid.org/0000-0002-2026-4156 Sri Wahyuni Fatma Department of Pharmacy, Faculty of Pharmacy, Universitas Andalas 06 28 2025 28 4 952 960 04 10 2023 08 10 2023 Copyright © 2010, Journal of Research in Pharmacy 2010 Journal of Research in Pharmacy

The liver—the center of metabolism and detoxification—is especially susceptible to damage from toxins and must be protected. This study aimed to evaluate the hepatoprotective effects of the ethyl acetate fraction from three Indonesian Artocarpus species (Artocarpus altilis, A. champeden, and A. heterophyllus) in rats with acute liver injury induced by carbon tetrachloride (CCl4). The hepatoprotective effect was assessed through biochemical parameters (AST, ALT, and ALP) and histological analysis of the liver of the test group and compared to the normal control group (Na CMC 0.5%) and the positive control group (silymarin 100 mg/kg). There was no significant difference between the AST, ALT, and ALP levels in the 500 mg dose group, the control and the silymarin group (p > 0.05). Histological analysis of the liver tissue showed a microscopic profile similar to that of the control and silymarin groups. This study showed that the ethyl acetate fraction of Artocarpus altilis, A. heterophyllus, and A. champeden has synergistic potential as a hepatoprotector against liver injury caused by CCl4. Consequently, the leaves of these species can be used as complementary medicine in treating acute liver damage.

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