j. res. pharm. Journal of Research in Pharmacy 2630-6344 Marmara University Pharmaceutical Analytical Chemistry Eczacılıkta Analitik Kimya Fullerene modified single-use electrodes as a convenient biosensor platform for electrochemical monitoring of drug- DNA interaction Ekşin Ece EGE ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ, TEMEL ECZACILIK BİLİMLERİ BÖLÜMÜ, ANALİTİK KİMYA ANABİLİM DALI https://orcid.org/0000-0002-4375-8386 Erdem Arzum EGE ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ, TEMEL ECZACILIK BİLİMLERİ BÖLÜMÜ, ANALİTİK KİMYA ANABİLİM DALI 06 28 2025 26 4 997 1006 02 08 2022 05 12 2022 Copyright © 2010, Journal of Research in Pharmacy 2010 Journal of Research in Pharmacy

Fullerene enriched disposable electrodes were developed and used as a biosensor platform for electrochemical monitoring of drug-DNA interaction. The surface of pencil graphite electrode (PGE) was modified with fullerene in order to enhance electrode surface for efficient dsDNA immobilization. The surface of fullerene modified PGEs (f-PGEs) was characterized using scanning electron microscopy (SEM), and electrochemical impedance spectroscopy (EIS). The behavior of dsDNA layer onto f-PGE surface was investigated by EIS measurements in Fe(CN6)4-/3- redox system. After optimization of several experimental parameters, the suitability of the constructed electrodes as a sensor platform for monitoring of drug-DNA interaction has been examined. The reproducibility of the biosensor was also tested in the presence of dsDNA immobilization. Consequently, the developed fullerene based biosensor offers suitable platform for the electrochemical analysis of drug-DNA interaction sensitively.

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