j. res. pharm. Journal of Research in Pharmacy 2630-6344 Marmara University Pharmacology and Pharmaceutical Sciences (Other) Eczacılık ve İlaç Bilimleri (Diğer) Investigation of the anticancer effects of some plant seed oils with medicinal uses https://orcid.org/0000-0003-4598-7593 Kurman Yener DÜZCE ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, TIBBİ BİYOLOJİ ANABİLİM DALI https://orcid.org/0000-0002-9517-3830 Dülger Görkem DÜZCE ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, TIBBİ BİYOLOJİ ANABİLİM DALI Pasin Özge Bezmialem University, Biostatistics Department 06 28 2025 27 4 1594 1604 12 27 2022 03 11 2023 Copyright © 2010, Journal of Research in Pharmacy 2010 Journal of Research in Pharmacy

Cancer is an important health problem and responsible for a significant number of deaths worldwide. Chemotherapy resistance and related side effects prevent cancer treatment from reaching the desired level. In this study, we aimed to investigate the anti-cancer properties of Carthamus tinctorius, Salvia hispanica and Linum usitatissimum seed oils on hepatocellular (HepG2), colorectal (HCT-116) and gastric carcinoma (AGS) cell lines. Human umbilical vein endothelial cells (HUVECs) were used as control cell group. Cell viability of HepG2, HCT-116, AGS and HUVECs was determined by MTT method after treatment with various concentrations of Carthamus tinctorius, Salvia hispanica and Linum usitatissimum. Colorimetric elisa kit was used for quantitative determination of caspase-3 activation. Anti-migratory effect of Carthamus tinctorius and Salvia hispanica were investigated by wound healing assay. Carthamus tinctorius, Salvia hispanica and Linum usitatissimum seed oils significantly inhibited the proliferation of cancer cells. There was an insignificant decrease in the viability of HUVEC cells after seed oils treatments. Migration of HepG2 and AGS cells significantly suppressed by treatment of Salvia hispanica and Carthamus tinctorius respectively. Salvia hispanica and Carthamus tinctorius significantly increased caspase-3 activation in cancer cells. Our results showed that Carthamus tinctorius, Salvia hispanica and Linum usitatissimum exhibited a selective cytotoxicity against HepG2, HCT-116 and AGS cells without damaging normal cells.

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