Inflammation can result from the introduction of foreign things into the body, such as bacteria or viruses.Inflammation activates macrophages and mast cells, which serve as immunological agents. The resultant hydrolysis ofvirgin coconut oil (HVCO) has an anti-inflammatory effect. This research aimed to determine how HVCO affects anti-inflammatory effects in vitro RAW 264.7 cells were activated against lipopolysaccharide. HVCO has anti-inflammatoryeffects determined by performing a live-cell viability assay using the MTT method [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide], IL- 6, TNF- α, IL-1β, iNOS, COX-2, and β-actin gene expression have been studiedutilizing reverse transcription-polymerase chain reaction (RT-PCR). The HVCO test results on RAW 264.7 cells with thecell viability test at concentrations (62.5 g/mL; 31.5 g/mL) showed the percentage of live cells (> 90%), namely (97.74 ±0.31; 102.31 ± 1.21) and assays using the expression of iNOS, TNF-α, IL-6, IL-1β, COX-2, and β-actin genes from HVCOin cells induced with LPS decreased the density value of HVCO, the expression of iNOS and IL-1β resulted in densityvalues the best (0.72±0.010) and (2.40±0.015), TNF-α (0.76±0.7633), IL-6 (1.16±0.010), COX-2 (0.98 ± 0.010), and β-actin(1,02± 0,010). This study showed that HVCO has anti-inflammatory actions on RAW 264.7 cells caused bylipopolysaccharide.