j. res. pharm. Journal of Research in Pharmacy 2630-6344 Marmara University Pharmaceutical Microbiology Farmasotik Mikrobiyoloji Biosynthesis of Magnesium Oxide Nanoparticles Using Opunita ficus-indica and Their Antifungal Effect Against Aspergillus Niger Alholy Taif Department of Biochemistry and Microbiology, College of Pharmacy, AL Baath University, Syria, Homs. https://orcid.org/0000-0001-6771-2211 Khaddam Walid Department of Biochemistry and Microbiology, Prof in the Faculty of Pharmacy, AL Baath University, Syria, Homs 06 28 2025 27 3 1188 1201 12 21 2022 01 28 2023 Copyright © 2010, Journal of Research in Pharmacy 2010 Journal of Research in Pharmacy

Magnesium oxide nanoparticles (MgO-NPs) were synthesis via green method using Opunita ficus indicaextract as reducing and covering agent. The optimal formula for the preparation of MgO-NPs was determined by UV-Vis and DLS (Opunita ficus -indica extracted by distilled water and ethanol solvent, magnesium nitrate salt (1mM), stirredat 70 °C for 24 h with pH= 9). UV-Vis analysis showed a peak at 300 nm, while DLS measured the hydrodynamicdiameter of the nanoparticles. FTIR results suggested that the polysaccharides, phenols and amines present in theextract might have been involved in the formation of MgO-NPs from Mg (NO3)2 as the reducing agent. Image J wasutilized to analyze the SEM results and determine the size, which was on average 99 nm, the shape of the nanoparticleswas spherical, and EDX spectrum confirmed the presence of magnesium (Mg). It was found that MgO-NPs are highlytoxic against Aspergillus niger. Which showed a gradual inhibitory effect when using the concentration of 0.5% and1.25%, and the inhibitory ability was 66.6%, 100% respectively, when using the poisoned food technique.

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