j. res. pharm. Journal of Research in Pharmacy 2630-6344 Marmara University 10.12991/jrespharm.1798051 Pharmacology and Pharmaceutical Sciences (Other) Eczacılık ve İlaç Bilimleri (Diğer) In vivo study: Effectiveness and safety of reverse micelle EGCG as a cervical anticancer treatment https://orcid.org/0009-0006-5802-7742 Rosita Noorma Pharmaceutical Science Department, Faculty of Pharmacy, Universitas Airlangga https://orcid.org/0009-0001-4827-1462 Rosalina Linda Adianti Pharmaceutical Science Department, Faculty of Pharmacy, Universitas Airlangga https://orcid.org/0000-0003-1658-0778 Miatmoko Andang Pharmaceutical Science Department, Faculty of Pharmacy, Universitas Airlangga https://orcid.org/0000-0002-5983-5614 Erawati Tristiana Pharmaceutical Science Department, Faculty of Pharmacy, Universitas Airlangga https://orcid.org/0000-0001-5929-4726 Yusuf Helmy Pharmaceutical Science Department, Faculty of Pharmacy, Universitas Airlangga Plumeriastuti Hani Division of Veterinary Pathology, Department of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga 11 02 2025 29 6 2431 2442 07 09 2024 09 02 2024 Copyright © 2010, Journal of Research in Pharmacy 2010 Journal of Research in Pharmacy

(-)-Epigallocatechin gallate (EGCG) is reported as inducing apoptosis in cervical cancer and recommended for topical delivery by means of reverse micelle (RM). This study aims to determine the in vivo effectiveness and safety of EGCG reverse micelles (EGCG_RM) as an anticancer agent compared with free EGCG in mice induced with Ethinyl Estradiol through intravaginal administration. In this study, the EGCG_RM were prepared by mixing the surfactant Tween 80 and Span 80 at an HLB value of 6 and characterized by physicochemical properties through FTIR, droplet measurements, particle size, polydispersity index, and zeta potential. The entrapment efficiency and drug loading values of EGCG were also determined. The in vivo effectiveness and safety of RM_EGCG were observed based on changes in body weight, organ morphology, and uterine tissue histopathology of the mice subject negative controls compared to the healthy controls post-intravaginal administration. Characteristics of the resulting EGCG-RM included: 100-200 nm in size, which was deemed appropriate for cancer therapy and demonstrated no morphological or histopathological change, as shown by the negative control resulting from Ethinyl Estradiol induction. The fact that they present no irritation can be seen from the healthy control group. EGCG_RM can increase the effectiveness of cervical anticancer activity and does not cause irritation after local administration via the intravaginal route.

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