Formulation and evaluation of acetazolamide loaded in–situ gel for the treatment of glaucoma

Year 2023, Volume: 27 Issue: 1, 74 - 85, 28.06.2025

Fulchan Ali Sk Habibullah , Yashwant Giri Amulyaratna Behera Biswaranjan Mohanty

Abstract

The present research work was proposed to develop a pH triggered in-situ gel formulation for the ocular delivery of acetazolamide to treat glaucoma. Carbopol-934, Carbopol 940, Hydroxypropyl methylcellulose (HPMC) K4M, and Sodium Alginate were chosen for the development of in-situ gel. Formulation with Carbopol 940 and sodium alginate was optimized by assessing the gelling capacity. The prepared in-situ gel formulations were thoroughly characterized for gelling time, gelation temperature, Fourier-transform infrared (FTIR) spectroscopy studies, rheological study, sterility testing, corneal drug permeation, ocular irritation test, and accelerated stability study. Ex-vivo corneal permeation study was performed using goat cornea. The result of transcorneal permeation of acetazolamide followed the Fickian diffusion process. The optimized formulations showed satisfactory gelling time (4.17-5.17 sec) and dissolution time (120 min). FTIR study confirms the compatibility between the polymers and acetazolamide. The sterility study showed a satisfactory result. After 72 h observation in the in-vivo rabbit eye irritation study, the eyes appeared normal. There were no significant changes in pH and drug content in the accelerated stability studies of the formulations.

Keywords

Acetazolamide , gelation time , carbopol-934&940 , sodium alginate , in-situ gel , release , viscosity

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