Time and Concentration Dependent Effects of Different Solvents on Proliferation of K562, HL60, HCT-116 and H929 Cell Lines

Year 2022, Volume: 26 Issue: 3, 494 - 501, 28.06.2025

Aslı Koc , Arzu Zeynep Karabay , Tülin Özkan , Zeliha Buyukbingol Fügen Aktan

Abstract

Many organic solvents are used in cell culture models to dissolve polar and non-polar chemical
compounds and drugs to test their activities. However, the solvents themselves can have cytotoxic effects on
cells. The degree of cytotoxic effect may vary according to the solvent used. The aim of this study is to determine
the time and concentration dependent effects of methanol, ethanol, dimethyl sulfoxide (DMSO), ethyl acetate
and acetone on colorectal cancer (HCT-116), chronic myeloid leukemia (K562), multiple myeloma (H929) and
acute promyelocytic leukemia (HL60) cell lines. According to our results, DMSO is the most toxic solvent and
ethyl acetate and methanol are least toxic solvents in all cells. IC50 value of Acetone is greater than 10% in K562,
HL60 and HCT-116 cells in all tested incubation times but in H929 cells IC50 value of acetone is decreased over
time. In conclusion, since most of these solvents are currently being used for treatment of cells with different
drugs and compounds in biochemical toxicology studies, it is essentially important to reveal their biochemical
and toxic effects to determine the optimum concentration to be used

Keywords

DMSO , Methanol , Acetone , Ethanol , cytotoxicity , cell culture , MTT , cancer

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