Research Article
BibTex RIS Cite
Year 2022, Volume: 41 Issue: 2, 143 - 149, 31.12.2022
https://doi.org/10.30782/jrvm.1161358

Abstract

Supporting Institution

İstanbul Üniversitesi- Cerrahpaşa Bilimsel Araştırma Projeleri Birimi

Project Number

BYP-2017-25470 ve BYP-2017-27877

References

  • 1. Donga S, Moteriya P, Pande J, Chanda S. Development of quality control parameters for the standardization of Pterocarpus santalinus Linn. F. leaf and stem. J Pharmacogn Phytochem. 2017; 6 (4): 242-252.
  • 2. Anlas C, Bakirel T, Ustun-Alkan F, et al. In vitro evaluation of the therapeutic potential of Anatolian kermes oak (Quercus coccifera L.) as an alternative wound healing agent. Ind Crops Prod. 2019; 137: 24-32.
  • 3. Akinboro A, Bakare AA. Cytotoxic and genotoxic effects of aqueous extracts of five medicinal plants on Allium cepa Linn. J Ethnopharmacol. 2007; 112(3): 470-475.
  • 4. Newman DJ, Cragg GM. Natural products as sources of new drugs over the last 25 years. J Nat Prod. 2007; 70: 461–477.
  • 5. Rodeiro I, Hernandez S, Morffi J, et al. Evaluation of genotoxicity and DNA protective effects of mangiferin, a glucosylxanthone isolated from Mangifera indica L. stem bark extract. Food Chem Toxicol. 2012; 50(9): 3360-3366.
  • 6. Demma J, Engidawork E, Hellman B. Potential genotoxicity of plant extracts used in Ethiopian traditional medicine. J Ethnopharmacol. 2009; 122(1): 136-142.
  • 7. Romero-Jimenez M, Campos-Sanchez J, Allana M, Munoz-Serrano A, Alonso-Moraga A. Genotoxicity and antigenotoxicity of some traditional medicinal herbs. Mutat Res. 2005; 585: 147–155.
  • 8. Oskay M, Sarı D. Antimicrobial screening of some Turkish medicinal plants. Pharm Biol. 2008; 45(3): 176-181.
  • 9. Koca-Caliskan U, Yilmaz I, Taslidere A, Yalcin FN, Aka C, Sekeroglu N. Cuscuta arvensis Beyr “dodder”: in vivo hepatoprotective effects against acetaminophen-induced hepatotoxicity in rats. J Med Food. 2018; 21(6): 625-631.
  • 10. Şekeroglu N, Koca U, Meraler SA. Geleneksel bir halk ilacı: İkşut. Yuz Yil Univ J Agric Sci. 2012; 22(1): 235-243.
  • 11. Küpeli E, Orhan İ, Küsmenoğlu Ş, Yeşilada E. Evaluation of anti-inflammatory and antinociceptive activity of five Anatolian Achillea species. Turk J Pharm Sci. 2007; 4(2): 89-99.
  • 12. Haliloglu Y, Ozek T, Tekin M, Goger F, Baser KHC, Ozek G. Phytochemicals, antioxidant, and antityrosinase activities of Achillea sivasica Çelik and Akpulat. Int J Food Prop.2017; 20(1): 693-706.
  • 13. Altundag E, Ozturk M. Ethnomedicinal studies on the plant resources of east Anatolia, Turkey. Procedia Soc Behav Sci. 2011; 19: 756-777.
  • 14. Koca U, Küpeli-Akkol E, Sekeroglu N. Evaluation of in vivo and in vitro biological activities of different extracts of Cuscuta arvensis. Nat Prod Commun. 2011; 6(10): 1433-1436.
  • 15. Sharififar F, Pournournohammadi S, Arabnejad M. Immunomodulatory activity of aqueous extract of Achiella wilhelmsii C. Koch in mice. Indian J Exp Biol. 2009; 47: 668-671.
  • 16. Majnooni M, Mohammadi-Farani A, Gholivand M, Nikbakht M, Bahrami GR. Chemical composition and anxiolytic evaluation of Achillea Wilhelmsii C. Koch essential oil in rat. Res Pharm Sci. 2013; 8(4): 269-275.
  • 17. Dadkhah A, Fatemi F, Ababzadeh S, Roshanaei K, Alipour M, Tabrizi BS. Potential preventive role of Iranian Achillea wilhelmsii C. Koch essential oils in acetaminophen-induced hepatotoxicity. Bot Stud. 2014; 55(1): 1-10.
  • 18. Azaz AD, Arabaci T, Sangun MK, Yildiz B. Composition and the in vitro Antimicrobial Activities of the Essential Oils of Achillea wilhelmsii C. Koch. and Achillea lycaonica Boiss & Heldr. Asian J Chem. 2008; 20(2): 1238-1244.
  • 19. ISO (International Standard Organization) 10993-5. Biological evaluation of medical Devices Part 5: Tests for in vitro cytotoxicity. http://nhiso.com/wp-content/uploads/2018/05/ISO-10993-5-2009.pdf. Accessed July 3, 2022.
  • 20. Tice RR, Agurell E, Anderson D, et al. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen. 2000; 35(3): 206-221.
  • 21. Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res. 1988; 175(1): 184-191.
  • 22. Singh S, Chattopadhyay P, Borthakur SK, Policegoudra R. Safety profile investigations of Meyna spinosa (Roxb.) and Oroxylum indicum (Linn.) extracts collected from Northeast India. Pharmacognosy Magazine. 2017; 13(4): 762- 768.
  • 23. Guncum E, Bakirel T, Anlas C, et al. The screening of the safety profile of polymeric based amoxicillin nanoparticles in various test systems. Toxicol Lett. 2021; 348: 1-9.
  • 24. Ghobadian Z, Ahmadi MRH, Rezazadeh L, Hosseini E, Kokhazadeh T, Ghavam S. In vitro evaluation of Achillea millefolium on the production and stimulation of human skin fibroblast cells (HFS-PI-16). Med Arch. 2015; 69(4): 212-217.
  • 25. Agar OT, Dikmen M, Ozturk N, Yilmaz MA, Temel H, Turkmenoglu FP. Comparative studies on phenolic composition, antioxidant, wound healing and cytotoxic activities of selected Achillea L. species growing in Turkey. Molecules. 2015; 20(10): 17976-18000.
  • 26. Sargazi S, Moudi M, Kooshkaki O, Mirinejad S, Saravani R. Hydro-alcoholic extract of Achillea Wilhelmsii C. Koch reduces the expression of cell death-associated genes while inducing DNA damage in HeLa cervical cancer cells. Iran J Med Sci. 2020; 45(5): 359- 367.
  • 27. Abedini MR, Paki S, Mohammadifard M, Foadoddini M, Vazifeshenas-Darmiyan K, Hosseini M. Evaluation of the in vivo and in vitro safety profile of Cuscuta epithymum ethanolic extract. Avicenna Journal of Phytomedicine. 2021; 11(6): 645-656.
  • 28. Rafińska K, Pomastowski P, Rudnicka J, et al. Effect of solvent and extraction technique on composition and biological activity of Lepidium sativum extracts. Food Chem. 2019; 289: 16-25.
  • 29. Praseeja RJ, Sreejith PS, Asha VV. Studies on the apoptosis inducing and cell cycle regulatory effect of Cuscuta reflexa Roxb chloroform extract on human hepatocellular carcinoma cell line, Hep 3B. Int J Appl Res Nat Prod. 2015; 8(2): 37-47.
  • 30. Riaz M, Bilal A, Ali MS, et al. Natural products from Cuscuta reflexa Roxb. with antiproliferation activities in HCT116 colorectal cell lines. Natural Prod Res. 2017; 31(5): 583-587.
  • 31. Diantini A, Subarnas A, Lestari K, et al. Kaempferol-3-O-rhamnoside isolated from the leaves of Schima wallichii Korth. inhibits MCF-7 breast cancer cell proliferation through activation of the caspase cascade pathway. Oncol Lett. 2012; 3(5): 1069-1072.
  • 32. El Yamani N, Collins AR, Rundén-Pran E, et al. In vitro genotoxicity testing of four reference metal nanomaterials, titanium dioxide, zinc oxide, cerium oxide and silver: towards reliable hazard assessment. Mutagenesis. 2017; 32(1): 117-126.
  • 33. Dokuparthi SK, Banerjee N, Kumar A, Singamaneni V, Giri AK, Mukhopadhyay S. Phytochemical investigation and evaluation of antimutagenic activity of the extract of Cuscuta reflexa Roxb by Ames Test. Int J Pharm Sci Res. 2014; 5(8): 3430- 3434.

In Vitro Cytotoxicity and Genotoxicity Screening of Cuscuta Arvensis Beyr. and Achillea Wilhelmsii C. Koch

Year 2022, Volume: 41 Issue: 2, 143 - 149, 31.12.2022
https://doi.org/10.30782/jrvm.1161358

Abstract

Plant-based compounds have been used for medicinal purposes since ancient times, as easily accessible and low-cost treatment options. Despite the widespread belief that plants are quite safe and devoid of side effects, scientific studies have revealed the toxicity potential of active components of plants on healthy cells. The present study was designed to investigate in vitro cytotoxicity and genotoxicity potential of Achillea wilhelmsii C. Koch and Cuscuta arvensis Beyr., which are frequently used in traditional medicine. In this context, cytotoxicity evaluation of the extracts was performed by MTT (3- [4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay. Our cytotoxicity results indicated that the extract from A. wilhelmsii did not affect the viability of fibroblasts at any of the concentrations, but rather significantly stimulated cell proliferation from a concentration of 25 µg/mL. On the other hand, the extract from C. arvensis significantly reduced the viability of fibroblasts at all concentrations tested. In the second part of this research, the DNA damaging potential of the extracts was investigated by in vitro comet assay at non-cytotoxic concentrations. A. wilhelmsii extract caused a significant increase in the percentage of DNA in the tail (%TDNA), which is considered an indicator of DNA damage, only at the highest concentration, while C. arvensis extract did not significantly affect %TDNA at concentrations tested. The results of the present study indicated that the methanolic extract from A. wilhelmsii may be considered safe up to a concentration of 100 μg/mL, however, the cytotoxicity potential of C. arvensis may be a factor limiting its safe use.

Project Number

BYP-2017-25470 ve BYP-2017-27877

References

  • 1. Donga S, Moteriya P, Pande J, Chanda S. Development of quality control parameters for the standardization of Pterocarpus santalinus Linn. F. leaf and stem. J Pharmacogn Phytochem. 2017; 6 (4): 242-252.
  • 2. Anlas C, Bakirel T, Ustun-Alkan F, et al. In vitro evaluation of the therapeutic potential of Anatolian kermes oak (Quercus coccifera L.) as an alternative wound healing agent. Ind Crops Prod. 2019; 137: 24-32.
  • 3. Akinboro A, Bakare AA. Cytotoxic and genotoxic effects of aqueous extracts of five medicinal plants on Allium cepa Linn. J Ethnopharmacol. 2007; 112(3): 470-475.
  • 4. Newman DJ, Cragg GM. Natural products as sources of new drugs over the last 25 years. J Nat Prod. 2007; 70: 461–477.
  • 5. Rodeiro I, Hernandez S, Morffi J, et al. Evaluation of genotoxicity and DNA protective effects of mangiferin, a glucosylxanthone isolated from Mangifera indica L. stem bark extract. Food Chem Toxicol. 2012; 50(9): 3360-3366.
  • 6. Demma J, Engidawork E, Hellman B. Potential genotoxicity of plant extracts used in Ethiopian traditional medicine. J Ethnopharmacol. 2009; 122(1): 136-142.
  • 7. Romero-Jimenez M, Campos-Sanchez J, Allana M, Munoz-Serrano A, Alonso-Moraga A. Genotoxicity and antigenotoxicity of some traditional medicinal herbs. Mutat Res. 2005; 585: 147–155.
  • 8. Oskay M, Sarı D. Antimicrobial screening of some Turkish medicinal plants. Pharm Biol. 2008; 45(3): 176-181.
  • 9. Koca-Caliskan U, Yilmaz I, Taslidere A, Yalcin FN, Aka C, Sekeroglu N. Cuscuta arvensis Beyr “dodder”: in vivo hepatoprotective effects against acetaminophen-induced hepatotoxicity in rats. J Med Food. 2018; 21(6): 625-631.
  • 10. Şekeroglu N, Koca U, Meraler SA. Geleneksel bir halk ilacı: İkşut. Yuz Yil Univ J Agric Sci. 2012; 22(1): 235-243.
  • 11. Küpeli E, Orhan İ, Küsmenoğlu Ş, Yeşilada E. Evaluation of anti-inflammatory and antinociceptive activity of five Anatolian Achillea species. Turk J Pharm Sci. 2007; 4(2): 89-99.
  • 12. Haliloglu Y, Ozek T, Tekin M, Goger F, Baser KHC, Ozek G. Phytochemicals, antioxidant, and antityrosinase activities of Achillea sivasica Çelik and Akpulat. Int J Food Prop.2017; 20(1): 693-706.
  • 13. Altundag E, Ozturk M. Ethnomedicinal studies on the plant resources of east Anatolia, Turkey. Procedia Soc Behav Sci. 2011; 19: 756-777.
  • 14. Koca U, Küpeli-Akkol E, Sekeroglu N. Evaluation of in vivo and in vitro biological activities of different extracts of Cuscuta arvensis. Nat Prod Commun. 2011; 6(10): 1433-1436.
  • 15. Sharififar F, Pournournohammadi S, Arabnejad M. Immunomodulatory activity of aqueous extract of Achiella wilhelmsii C. Koch in mice. Indian J Exp Biol. 2009; 47: 668-671.
  • 16. Majnooni M, Mohammadi-Farani A, Gholivand M, Nikbakht M, Bahrami GR. Chemical composition and anxiolytic evaluation of Achillea Wilhelmsii C. Koch essential oil in rat. Res Pharm Sci. 2013; 8(4): 269-275.
  • 17. Dadkhah A, Fatemi F, Ababzadeh S, Roshanaei K, Alipour M, Tabrizi BS. Potential preventive role of Iranian Achillea wilhelmsii C. Koch essential oils in acetaminophen-induced hepatotoxicity. Bot Stud. 2014; 55(1): 1-10.
  • 18. Azaz AD, Arabaci T, Sangun MK, Yildiz B. Composition and the in vitro Antimicrobial Activities of the Essential Oils of Achillea wilhelmsii C. Koch. and Achillea lycaonica Boiss & Heldr. Asian J Chem. 2008; 20(2): 1238-1244.
  • 19. ISO (International Standard Organization) 10993-5. Biological evaluation of medical Devices Part 5: Tests for in vitro cytotoxicity. http://nhiso.com/wp-content/uploads/2018/05/ISO-10993-5-2009.pdf. Accessed July 3, 2022.
  • 20. Tice RR, Agurell E, Anderson D, et al. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen. 2000; 35(3): 206-221.
  • 21. Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res. 1988; 175(1): 184-191.
  • 22. Singh S, Chattopadhyay P, Borthakur SK, Policegoudra R. Safety profile investigations of Meyna spinosa (Roxb.) and Oroxylum indicum (Linn.) extracts collected from Northeast India. Pharmacognosy Magazine. 2017; 13(4): 762- 768.
  • 23. Guncum E, Bakirel T, Anlas C, et al. The screening of the safety profile of polymeric based amoxicillin nanoparticles in various test systems. Toxicol Lett. 2021; 348: 1-9.
  • 24. Ghobadian Z, Ahmadi MRH, Rezazadeh L, Hosseini E, Kokhazadeh T, Ghavam S. In vitro evaluation of Achillea millefolium on the production and stimulation of human skin fibroblast cells (HFS-PI-16). Med Arch. 2015; 69(4): 212-217.
  • 25. Agar OT, Dikmen M, Ozturk N, Yilmaz MA, Temel H, Turkmenoglu FP. Comparative studies on phenolic composition, antioxidant, wound healing and cytotoxic activities of selected Achillea L. species growing in Turkey. Molecules. 2015; 20(10): 17976-18000.
  • 26. Sargazi S, Moudi M, Kooshkaki O, Mirinejad S, Saravani R. Hydro-alcoholic extract of Achillea Wilhelmsii C. Koch reduces the expression of cell death-associated genes while inducing DNA damage in HeLa cervical cancer cells. Iran J Med Sci. 2020; 45(5): 359- 367.
  • 27. Abedini MR, Paki S, Mohammadifard M, Foadoddini M, Vazifeshenas-Darmiyan K, Hosseini M. Evaluation of the in vivo and in vitro safety profile of Cuscuta epithymum ethanolic extract. Avicenna Journal of Phytomedicine. 2021; 11(6): 645-656.
  • 28. Rafińska K, Pomastowski P, Rudnicka J, et al. Effect of solvent and extraction technique on composition and biological activity of Lepidium sativum extracts. Food Chem. 2019; 289: 16-25.
  • 29. Praseeja RJ, Sreejith PS, Asha VV. Studies on the apoptosis inducing and cell cycle regulatory effect of Cuscuta reflexa Roxb chloroform extract on human hepatocellular carcinoma cell line, Hep 3B. Int J Appl Res Nat Prod. 2015; 8(2): 37-47.
  • 30. Riaz M, Bilal A, Ali MS, et al. Natural products from Cuscuta reflexa Roxb. with antiproliferation activities in HCT116 colorectal cell lines. Natural Prod Res. 2017; 31(5): 583-587.
  • 31. Diantini A, Subarnas A, Lestari K, et al. Kaempferol-3-O-rhamnoside isolated from the leaves of Schima wallichii Korth. inhibits MCF-7 breast cancer cell proliferation through activation of the caspase cascade pathway. Oncol Lett. 2012; 3(5): 1069-1072.
  • 32. El Yamani N, Collins AR, Rundén-Pran E, et al. In vitro genotoxicity testing of four reference metal nanomaterials, titanium dioxide, zinc oxide, cerium oxide and silver: towards reliable hazard assessment. Mutagenesis. 2017; 32(1): 117-126.
  • 33. Dokuparthi SK, Banerjee N, Kumar A, Singamaneni V, Giri AK, Mukhopadhyay S. Phytochemical investigation and evaluation of antimutagenic activity of the extract of Cuscuta reflexa Roxb by Ames Test. Int J Pharm Sci Res. 2014; 5(8): 3430- 3434.
There are 33 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Research Articles
Authors

Ceren Anlaş 0000-0002-7997-016X

Tülay Bakırel 0000-0001-5805-2178

Ufuk Koca Çalışkan 0000-0002-5216-7588

Ceylan Dönmez 0000-0002-8005-4425

Fulya Üstün Alkan 0000-0003-1782-9467

Oya Üstüner Keleş 0000-0002-4354-8207

Project Number BYP-2017-25470 ve BYP-2017-27877
Publication Date December 31, 2022
Acceptance Date December 24, 2022
Published in Issue Year 2022 Volume: 41 Issue: 2

Cite

APA Anlaş, C., Bakırel, T., Çalışkan, U. K., Dönmez, C., et al. (2022). In Vitro Cytotoxicity and Genotoxicity Screening of Cuscuta Arvensis Beyr. and Achillea Wilhelmsii C. Koch. Journal of Research in Veterinary Medicine, 41(2), 143-149. https://doi.org/10.30782/jrvm.1161358
AMA Anlaş C, Bakırel T, Çalışkan UK, Dönmez C, Üstün Alkan F, Üstüner Keleş O. In Vitro Cytotoxicity and Genotoxicity Screening of Cuscuta Arvensis Beyr. and Achillea Wilhelmsii C. Koch. J Res Vet Med. December 2022;41(2):143-149. doi:10.30782/jrvm.1161358
Chicago Anlaş, Ceren, Tülay Bakırel, Ufuk Koca Çalışkan, Ceylan Dönmez, Fulya Üstün Alkan, and Oya Üstüner Keleş. “In Vitro Cytotoxicity and Genotoxicity Screening of Cuscuta Arvensis Beyr. And Achillea Wilhelmsii C. Koch”. Journal of Research in Veterinary Medicine 41, no. 2 (December 2022): 143-49. https://doi.org/10.30782/jrvm.1161358.
EndNote Anlaş C, Bakırel T, Çalışkan UK, Dönmez C, Üstün Alkan F, Üstüner Keleş O (December 1, 2022) In Vitro Cytotoxicity and Genotoxicity Screening of Cuscuta Arvensis Beyr. and Achillea Wilhelmsii C. Koch. Journal of Research in Veterinary Medicine 41 2 143–149.
IEEE C. Anlaş, T. Bakırel, U. K. Çalışkan, C. Dönmez, F. Üstün Alkan, and O. Üstüner Keleş, “In Vitro Cytotoxicity and Genotoxicity Screening of Cuscuta Arvensis Beyr. and Achillea Wilhelmsii C. Koch”, J Res Vet Med, vol. 41, no. 2, pp. 143–149, 2022, doi: 10.30782/jrvm.1161358.
ISNAD Anlaş, Ceren et al. “In Vitro Cytotoxicity and Genotoxicity Screening of Cuscuta Arvensis Beyr. And Achillea Wilhelmsii C. Koch”. Journal of Research in Veterinary Medicine 41/2 (December 2022), 143-149. https://doi.org/10.30782/jrvm.1161358.
JAMA Anlaş C, Bakırel T, Çalışkan UK, Dönmez C, Üstün Alkan F, Üstüner Keleş O. In Vitro Cytotoxicity and Genotoxicity Screening of Cuscuta Arvensis Beyr. and Achillea Wilhelmsii C. Koch. J Res Vet Med. 2022;41:143–149.
MLA Anlaş, Ceren et al. “In Vitro Cytotoxicity and Genotoxicity Screening of Cuscuta Arvensis Beyr. And Achillea Wilhelmsii C. Koch”. Journal of Research in Veterinary Medicine, vol. 41, no. 2, 2022, pp. 143-9, doi:10.30782/jrvm.1161358.
Vancouver Anlaş C, Bakırel T, Çalışkan UK, Dönmez C, Üstün Alkan F, Üstüner Keleş O. In Vitro Cytotoxicity and Genotoxicity Screening of Cuscuta Arvensis Beyr. and Achillea Wilhelmsii C. Koch. J Res Vet Med. 2022;41(2):143-9.