kujes Kastamonu University Journal of Engineering and Sciences 2667-8209 Kastamonu University 10.55385/kastamonujes.1333878 Biomaterial Biyomateryaller Halobacillus trueperi CT7: A Spore-Forming, Gelatinase Producing, Salt-Tolerant Bacteria Isolated from Çankırı Salt Mine https://orcid.org/0000-0003-3942-5703 Kesbiç Fevziye Işıl KASTAMONU ÜNİVERSİTESİ https://orcid.org/0000-0003-4332-522X Gültepe Nejdet ATATURK UNIVERSITY https://orcid.org/0000-0001-8804-5323 Tüzemen Naci KASTAMONU ÜNİVERSİTESİ 12 26 2023 9 2 46 52 07 28 2023 11 15 2023 Copyright © 2015, Kastamonu University Journal of Engineering and Sciences 2015 Kastamonu University Journal of Engineering and Sciences

Halophilic microorganisms are an extreme group of organisms that can spread at high salt concentrations, and a significant part of them consists of halophilic bacteria. Salt mines are important sources where halophilic bacteria are detected. In this study, Halobacillus trueperi CT7, a halophilic bacterium, was isolated from Çankırı Salt Mine. It was determined that the obtained strain showed 98.1% similarity with Halobacillus trueperi by DNA isolation and sequence analysis as well as biochemical analysis. In addition, two-dimensional (scanning electron microscopy) and three-dimensional (atomic force microscopy) images of Halobacillus trueperi were performed to reveal the cell morphology. In order to determine the industrial use potential of the microorganism, the minimum and maximum salt concentrations, temperature and pH ranges in which the species can grow, as well as the enzyme activities, where the species can grow, were determined qualitatively. The interest in halophilic organisms for their use in extreme industrial processes is increasing day by day. It is thought that this study will contribute to future studies on halophilic bacteria.

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