Kastamonu University Journal of Forestry Faculty

1303-2399 1309-4181

Kastamonu University

10.17475/kastorman.263875

Kastamonu ilinden toplanan bazı yabani mantarların in vitro koşullar altında biyoaktif özellikleri

In Vitro Bioactive Properties of Some Wild Mushrooms Collected from Kastamonu Province

Yıldız

Sibel

Yılmaz

Ayşenur

Can

Zehra

11 27 2017

17 3 523 530 11 04 2016 11 21 2017

2001

Kastamonu University Journal of Forestry Faculty

koşullar altında biyoaktif özellikleriÖzetÇalışmanınamacı: Buçalışmada Kastamonu ilinden toplanan Cantharellus cibarius Fr., Craterellus cornucopioides (L.) Pers., Hydnum rufescens Pers. ve Macrolepiota procera (Scop. ex Fr.)Sing. yabani mantarlarının protein ve toplam fenolikiçerik miktarları ile antioksidan ve antimikrobiyal özellikleri belirlenmiştir.Çalışma alanı: Yabani mantarlar Türkiye’nin Kastamonu ilinden toplanmıştır.Materyalve Yöntem: Protein içeriği; Dumas metodu, toplam fenol içeriği;Folin–Ciocalteu yöntemi, antioksidan özellik; demir indirgeyici güç (FRAP)metodu ve antimikrobiyal özellik ise Escherichiacoli ATCC 25922, Staphylococcusaureus ATCC 25923, Enterococcusfaecalis ATCC 29212, Klebsiellapneumoniae ATCC 13883, Salmonellatyphimurium ATCC 14028, Acinetobacterhaemolyticus ATCC 19002 ve Proteusmirabilis ATCC 7002’e karşı agar kuyucuk difüzyon yöntemi kullanılarakhesaplanmıştır.Sonuçlar: Mantarların protein içerikleri%9.94 ile %27.46 arasında, toplam polifenol miktarları 0.592±0.004–1.624±0.026mg GAE g-1 aralığında ve antioksidan özellikleri0.896±0.003-4.245±0.042 μmol FeSO.7H2O g-1 aralığındadeğişmiştir. En yüksek protein miktarı,en yüksek toplam fenol miktarı ve en yüksek antioksidan özellik M. procera’da tespit edilmiştir. M procera ve H. rufescens mantarları Klebsiellapneumoniae ve Escherichia coli’ekarşı inhibitor etki göstermiştir. Araştırma vurguları: Çalışılan mantarlararasında, M. procera mantarıbiyoaktif özellikler yönünden en iyi değerleri göstermiştir dolayısıyla bumantar ileriki çalışmalar için umut vaad edicidir.

Aim of study: the protein and total phenolic contents, antioxidant and antimicrobialproperties of some wild mushrooms (Cantharelluscibarius Fr., Craterellus cornucopioides (L.)Pers., Hydnum rufescens Pers. and Macrolepiota procera (Scop. ex Fr.)Sing.) collected from Kastamonu province, in Turkeywas determined. Area of study: Wild mushrooms were collected from Kastamonu province,Turkey. Material and Methods: Dumas and Folin–Ciocalteu methods were used to determine the protein andthe total phenolic contents, respectively. The antioxidant activities of themushrooms were determined using the Ferric-reducing antioxidant power (FRAP). The antimicrobial activities were evaluatedusing the agar well diffusion method against the test organisms; Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Klebsiella pneumoniae ATCC 13883, Salmonella typhimurium ATCC 14028, Acinetobacter haemolyticus ATCC 19002and Proteus mirabilis ATCC 7002.Main results: While the protein contentsand the total polyphenol amounts of mushrooms were ranging between 9.94-27.46%and 0.592±0.004-1.624±0.026 mg GAE g-1, respectively, the range ofantioxidant activities were determined to range between 0.896±0.003-4.245±0.042μmol FeSO.7H2O g-1. The highest protein and the total phenoliccontent as well as the antioxidantproperties were detected in M.procera. H. rufescens and M. procera mushrooms showed inhibitoryeffect against in vitro growth of Klebsiellapneumoniae and Escherichia coli. Research highlights: Among theinvestigated mushrooms, M. procera showedthe best analysis values in bioactive properties and so this mushroom can beevaluated for further studies

Antioxidant Antimicrobial Mushroom Protein Total phenolic content

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