Türkiye’deki Bazı Melanoleuca türlerinin (Fungi: Basidiomycota) Filogenisi ve Yeni bir Kayıt Olarak Melanoleuca angelesiana A.H. Sm. Türünün Tanımlanması

Year 2018, Volume: 18 Issue: 3, 314 - 326, 28.12.2018

Aysenur Kalmer İsmail Acar Ayten Dızkırıcı Tekpınar

https://doi.org/10.17475/kastorman.499076

Cited By: 4

Abstract

Çalışmanın Amacı:
Bu çalışmanın amacı toplanan Melanoleuca
örneklerinin morfolojik karakterlerle tanımlanması, ITS ve LSU DNA sekanslarını
kullanarak türlerin filogenetik ilişkilerinin belirlenmesi ve Melanoleuca
cinsinin alt cins sayısının teyit edilmesidir.

Çalışma Alanı:
Örnekler Türkiye'nin farklı bölgelerinden toplanmış ve çalışma Van Yüzüncü Yıl
Üniversitesi Moleküler Biyoloji ve Genetik Bölümü'nde gerçekleştirilmiştir.

Material ve Yöntemler:
On dört türü temsil eden yirmi iki örnek kullanılmışır. Şapka, sap, lamel
yapıları ve bazidya, sistidya, spor yapıları sırasıyla makroskopik ve
mikroskopik özellikler olarak kullanılmıştır. Nükleer ribozomal iç aralayıcı
bölge (nrITS) ve ribosomal en büyük alt birim (LSU) moleküler analizler için
amplifiye edilmiştir.

Ana sonuçlar:
Bu çalışmada, Türkiye'den bazı Melanoleuca
türlerinin taksonomik konumları hem morfolojik karakterleri hemde iki farklı
nükleer DNA bölgesinin sekansları ile değerlendirilmiştir. nrITS bölgesi
(ITS1’de 112, 5,8S’de 1, ve ITS2’de 95), LSU bölgesine (46 değişken alan)
kıyasla daha bilgilendirici olmuştur. ITS bölgesinin analizleri Melanoluca cinsinin monofletik olduğunu
ve Melanoleuca ve Urticocystis olarak iki alt cinse
ayrıldığını göstermiştir.

Araştırma konuları:
İncelenen türler içerisinde Melanoleuca
angelesiana Türkiye mikobiyotası için ilk defa rapor edilmiştir. 

Keywords

ITS, LSU, Melanoleuca, Fungal taksonomi, Yeni kayıt

Phylogeny of Some Melanoleuca Species (Fungi: Basidiomycota) in Turkey and Identification of Melanoleuca angelesiana A.H. Sm. As a First Record

Abstract

Aim of Study: The purposes of the present study are to describe
and identify all collected Melanoleuca
samples using morphological characters, determine phylogenetic relationships
among species using DNA sequences of ITS and LSU regions and check the number
of subgenera of Melanoleuca genus.

Area of Study: Samples were collected from different parts
of Turkey and the study was conducted at the Department of Molecular Biology
and Genetics in Van Yüzüncü Yıl University.

Material and
Methods: Twenty-two samples, representatives of fourteen species, were used.
Structures of pileus, stipe, lamellae and basidia, cystidia, spores were used
as macroscopic and microscopic features, respectively. The nuclear ribosomal
internal transcribed spacer region (nrITS) and largest subunit (LSU) were
amplified for molecular analyses.

Main results: Taxonomic
positions of some Melanoleuca species
from Turkey were evaluated based on both morphological characters and sequences
of two different nuclear DNA regions. nrITS region was more informative (112
variable sites in ITS1, 1 in 5.8S and 95 in ITS2 subregions) compared to LSU
(46 variable sites). Analyses of ITS region revealed that the genus Melanoleuca was monophyletic and divided
into two subgenera as Melanoleuca and Urticocystis.

Research
highlights: Within studied species, Melanoleuca
angelesiana was firstly reported for mycobiota of Turkey

Keywords

ITS, LSU, Melanoleuca, Fungal taxonomy, New record

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