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Comparative Antioxidant Capacity and Enzyme Inhibitory Effect of Extracts from Prunus avium Leaves

Year 2020, Volume: 20 Issue: 3, 234 - 242, 30.12.2020
https://doi.org/10.17475/kastorman.849538

Abstract

Aim of study: This study aimed to investigate the antioxidant capacity and enzyme inhibitory effect of different solvent extracts obtained from Prunus avium leaves.
Material and methods: The antioxidant capacity were evaluated using in vitro assays including DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum and metal chelating activity. Enzyme inhibitory effects were screened against AChE, BChE, α-amylase, α-glucosidase and tyrosinase enzyme. Additionally, total phenolic and flavonoid content were determined spectrophotometrically.
Main results: The water extract yielded the highest phenolic content (66.65 mgGAE/g). In antioxidant assays, water extract exhibited strong antioxidant capacity (with exception of metal chelating activity). Methanol extract showed the highest enzyme inhibitory effect compared to other extracts.
Highlights: This study proved that P. avium leaves have the potential for the development of new phytopharmaceuticals or food additives.

References

  • Acero, N., Gradillas, A., Beltran, M., García, A. & Mingarro, D.M. (2019). Comparison of phenolic compounds profile and antioxidant properties of different sweet cherry (Prunus avium L.) varieties. Food Chemistry, 279, 260-271.
  • Asghari, B., Mafakheri, S., Zarrabi, M.M., Erdem, S. A., Orhan, I.E., & Bahadori, M.B. (2019). Therapeutic target enzymes inhibitory potential, antioxidant activity, and rosmarinic acid content of Echium amoenum. South African Journal of Botany, 120, 191-197.
  • Azwanida, N. N. (2015). A review on the extraction methods use in medicinal plants, principle, strength and limitation. Medicinal & Aromatic Plants, 4(196), 2167-0412.
  • Bahadori, M.B., Zengin, G., Bahadori, S., Dinparast, L., & Movahhedin, N. (2018). Phenolic composition and functional properties of wild mint (Mentha longifolia var. calliantha (Stapf) Briq.). International Journal of Food Properties, 21(1), 183-193.
  • Banerjee, J., Singh, R., Vijayaraghavan, R., MacFarlane, D., Patti, A.F. & Arora, A. (2017). Bioactives from fruit processing wastes: Green approaches to valuable chemicals. Food Chemistry, 225, 10-22.
  • Chai, W.M., Huang, Q., Lin, M.Z., Ou-Yang, C., Huang, W.Y., Wang, Y.X., Xu, K.L. & Feng, H.L. (2018). Condensed tannins from longan bark as inhibitor of tyrosinase: structure, activity, and mechanism. Journal of Agricultural and Food Chemistry, 66(4), 908-917.
  • Dziadek, K., Kopeć, A. & Tabaszewska, M. (2019). Potential of sweet cherry (Prunus avium L.) by-products: Bioactive compounds and antioxidant activity of leaves and petioles. European Food Research and Technology, 245, 763–772.
  • Dziadek, K., Kopeć, A. & Czaplicki, S. (2018). The petioles and leaves of sweet cherry (Prunus avium L.) as a potential source of natural bioactive compounds. European Food Research and Technology, 244(8), 1415–1426.
  • Gonçalves, B., Landbo, A.K., Knudsen, D., Silva, A.P., Moutinho-Pereira, J., Rosa, E. & Meyer, A.S. (2004). Effect of ripeness and postharvest storage on the phenolic profiles of cherries (Prunus avium L.). Journal of Agricultural and Food Chemistry, 52(3), 523-530.
  • Grochowski, D.M., Uysal, S., Zengin, G. & Tomczyk, M. (2019). In vitro antioxidant and enzyme inhibitory properties of Rubus caesius L. International Journal of Environmental Health Research, 29(3), 237-245.
  • Jesus, F., Gonçalves, A.C., Alves, G. & Silva, L.R. (2019). Exploring the phenolic profile, antioxidant, antidiabetic and anti-hemolytic potential of Prunus avium vegetal parts. Food Research International, 116, 600-610.
  • Kelebek, H. & Selli, S. (2011). Evaluation of chemical constituents and antioxidant activity of sweet cherry (Prunus avium L.) cultivars. International Journal of Food Science & Technology, 46(12), 2530-2537.
  • Kent, K., Charlton, K.E., Jenner, A. & Roodenrys, S. (2016). Acute reduction in blood pressure following consumption of anthocyanin-rich cherry juice may be dose-interval dependant: a pilot cross-over study. International Journal of Food Sciences and Nutrition, 67(1), 47-52.
  • Li, H. Y., Hao, Z. B., Wang, X. L., Huang, L. & Li, J.P. (2009). Antioxidant activities of extracts and fractions from Lysimachia foenum-graecum Hance. Bioresource Technology, 100(2), 970-974.
  • Masondo, N. A., Stafford, G. I., Aremu, A.O. & Makunga, N.P. (2019). Acetylcholinesterase inhibitors from southern African plants: An overview of ethnobotanical, pharmacological potential and phytochemical research including and beyond Alzheimer's disease treatment. South African Journal of Botany, 120, 39-64.
  • McCune, L.M., Kubota, C., Stendell-Hollis, N.R. & Thomson, C.A. (2010). Cherries and health: a review. Critical Reviews in Food Science and Nutrition, 51(1), 1-12.
  • Mollica, A., Zengin, G., Durdagi, S., Ekhteiari Salmas, R., Macedonio, G., Stefanucci, A., Dimmito, M.P. & Novellino, E. (2019). Combinatorial peptide library screening for discovery of diverse α-glucosidase inhibitors using molecular dynamics simulations and binary QSAR models. Journal of Biomolecular Structure and Dynamics, 37(3), 726-740.
  • Mollica, A., Zengin, G., Stefanucci, A., Ferrante, C., Menghini, L., Orlando, G., Brunetti, L., Locatelli, M., Dimmito, M.P., Novellino, E., Wakeel, O.K., Ogundeji, M.O., Onaolapo, A.Y. & Onaolapo, O.J. (2018). Nutraceutical potential of Corylus avellana daily supplements for obesity and related dysmetabolism. Journal of Functional Foods, 47, 562-574.
  • Murayyan, A.I., Manohar, C.M., Hayward, G. & Neethirajan, S. (2017). Antiproliferative activity of Ontario grown onions against colorectal adenocarcinoma cells. Food Research International, 96, 12-18.
  • Nawirska-Olszańska, A., Kolniak-Ostek, J., Oziembłowski, M., Ticha, A., Hyšpler, R., Zadak, Z., Zidova, P. & Paprstein, F. (2017). Comparison of old cherry cultivars grown in Czech Republic by chemical composition and bioactive compounds. Food Chemistry, 228(1), 136-142.
  • Rauf, A. & Jehan, N. (2017). Natural products as a potential enzyme inhibitors from medicinal plants. In Enzyme Inhibitors and Activators, 165-177.
  • Scalbert, A., Johnson, I.T. & Saltmarsh, M. (2005). Polyphenols: antioxidants and beyond. The American Journal of Clinical Nutrition, 81(1), 215-217.
  • Serradilla, M.J., Martín, A., Ruiz-Moyano, S., Hernández, A., López-Corrales, M. & de Guía Córdoba, M. (2012). Physicochemical and sensorial characterisation of four sweet cherry cultivars grown in Jerte Valley (Spain). Food Chemistry, 133(4), 1551-1559.
  • Średnicka-Tober, D., Ponder, A., Hallmann, E., Głowacka, A. & Rozpara, E. (2019). The profile and content of polyphenols and carotenoids in local and commercial sweet cherry fruits (Prunus avium L.) and their antioxidant activity in vitro. Antioxidants, 8(11), 534.
  • Usenik, V., Fabčič, J. & Štampar, F. (2008). Sugars, organic acids, phenolic composition and antioxidant activity of sweet cherry (Prunus avium L.). Food Chemistry, 107(1), 185-192.
  • Uysal, A., Ozer, O.Y., Zengin, G., Stefanucci, A., Mollica, A., Picot-Allain, C. M. N. & Mahomoodally, M. F. (2019). Multifunctional approaches to provide potential pharmacophores for the pharmacy shelf: Heracleum sphondylium L. subsp. ternatum (Velen.) Brummitt. Computational Biology and Chemistry, 78, 64-73.
  • Uysal, S., Zengin, G., Locatelli, M., Bahadori, M. B., Mocan, A., Bellagamba, G., De Luca, E., Mollica, A. & Aktumsek, A. (2017). Cytotoxic and enzyme inhibitory potential of two Potentilla species (P. speciosa L. and P. reptans Willd.) and their chemical composition. Frontiers in Pharmacology, 8, 290.
  • Uysal, S. & Aktumsek, A. (2015). A phytochemical study on Potentilla anatolica: an endemic Turkish plant. Industrial Crops and Products, 76, 1001-1007.
  • Wu, T., Tang, Q., Yu, Z., Gao, Z., Hu, H., Chen, W., Zheng, X. & Yu, T. (2014). Inhibitory effects of sweet cherry anthocyanins on the obesity development in C57BL/6 mice. International Journal of Food Sciences and Nutrition, 65(3), 351-359.
  • Zengin, G., Stefanucci, A., Rodrigues, M.J., Mollica, A., Custodio, L., Aumeeruddy, M.Z., & Mahomoodally, M. F. (2019). Scrophularia lucida L. as a valuable source of bioactive compounds for pharmaceutical applications: in vitro antioxidant, anti-inflammatory, enzyme inhibitory properties, in silico studies, and HPLC profiles. Journal of Pharmaceutical and Biomedical Analysis, 162, 225-233.

Prunus avium Yapraklarından Elde Edilen Ekstraktların Karşılaştırılmalı Antioksidan Kapasitesi ve Enzim Inhibisyon Etkisi

Year 2020, Volume: 20 Issue: 3, 234 - 242, 30.12.2020
https://doi.org/10.17475/kastorman.849538

Abstract

Çalışmanın amacı: Bu çalışmanın amacı Prunus avium yapraklarından elde edilen farklı çözücü ekstraktlarının antioksidan kapasitesini ve enzim inhibitör aktivitesini araştırmaktır.
Materyal ve yöntem: Antioksidan kapasite DPPH, ABTS, CUPRAC, FRAP, fosfomolibdat ve metal şelatlamayı içeren in vitro deneylerle değerlendirildi. Enzim inhibitor ektisi AChE, BChE, α-amilaz, α-glukozidaz ve tirozinaz enzimlerine karşı tarandı. Ek olarak, total fenolik ve flavonoid içerik spektrofotometrik olarak belirlendi.
Temel sonuçlar: Su ekstraktı en yüksek fenolik içeriğe sahiptir (66.65 mgGAE/g). Antioksidan deneylerde, su ekstraktı güçlü antioksidan kapasitesi sergiledi (metal şelatlama aktivitesi istisna olarak). Metanol ekstraktı diğer ekstraktlarla karşılaştırıldığında en yüksek enzim inhibitor etki gösterdi.
Araştırma vurguları: Bu çalışma P. avium yapraklarının yeni fitofarmasötikler veya gıda katkı maddelerinin geliştirme potansiyeline sahip olduğunu kanıtladı

References

  • Acero, N., Gradillas, A., Beltran, M., García, A. & Mingarro, D.M. (2019). Comparison of phenolic compounds profile and antioxidant properties of different sweet cherry (Prunus avium L.) varieties. Food Chemistry, 279, 260-271.
  • Asghari, B., Mafakheri, S., Zarrabi, M.M., Erdem, S. A., Orhan, I.E., & Bahadori, M.B. (2019). Therapeutic target enzymes inhibitory potential, antioxidant activity, and rosmarinic acid content of Echium amoenum. South African Journal of Botany, 120, 191-197.
  • Azwanida, N. N. (2015). A review on the extraction methods use in medicinal plants, principle, strength and limitation. Medicinal & Aromatic Plants, 4(196), 2167-0412.
  • Bahadori, M.B., Zengin, G., Bahadori, S., Dinparast, L., & Movahhedin, N. (2018). Phenolic composition and functional properties of wild mint (Mentha longifolia var. calliantha (Stapf) Briq.). International Journal of Food Properties, 21(1), 183-193.
  • Banerjee, J., Singh, R., Vijayaraghavan, R., MacFarlane, D., Patti, A.F. & Arora, A. (2017). Bioactives from fruit processing wastes: Green approaches to valuable chemicals. Food Chemistry, 225, 10-22.
  • Chai, W.M., Huang, Q., Lin, M.Z., Ou-Yang, C., Huang, W.Y., Wang, Y.X., Xu, K.L. & Feng, H.L. (2018). Condensed tannins from longan bark as inhibitor of tyrosinase: structure, activity, and mechanism. Journal of Agricultural and Food Chemistry, 66(4), 908-917.
  • Dziadek, K., Kopeć, A. & Tabaszewska, M. (2019). Potential of sweet cherry (Prunus avium L.) by-products: Bioactive compounds and antioxidant activity of leaves and petioles. European Food Research and Technology, 245, 763–772.
  • Dziadek, K., Kopeć, A. & Czaplicki, S. (2018). The petioles and leaves of sweet cherry (Prunus avium L.) as a potential source of natural bioactive compounds. European Food Research and Technology, 244(8), 1415–1426.
  • Gonçalves, B., Landbo, A.K., Knudsen, D., Silva, A.P., Moutinho-Pereira, J., Rosa, E. & Meyer, A.S. (2004). Effect of ripeness and postharvest storage on the phenolic profiles of cherries (Prunus avium L.). Journal of Agricultural and Food Chemistry, 52(3), 523-530.
  • Grochowski, D.M., Uysal, S., Zengin, G. & Tomczyk, M. (2019). In vitro antioxidant and enzyme inhibitory properties of Rubus caesius L. International Journal of Environmental Health Research, 29(3), 237-245.
  • Jesus, F., Gonçalves, A.C., Alves, G. & Silva, L.R. (2019). Exploring the phenolic profile, antioxidant, antidiabetic and anti-hemolytic potential of Prunus avium vegetal parts. Food Research International, 116, 600-610.
  • Kelebek, H. & Selli, S. (2011). Evaluation of chemical constituents and antioxidant activity of sweet cherry (Prunus avium L.) cultivars. International Journal of Food Science & Technology, 46(12), 2530-2537.
  • Kent, K., Charlton, K.E., Jenner, A. & Roodenrys, S. (2016). Acute reduction in blood pressure following consumption of anthocyanin-rich cherry juice may be dose-interval dependant: a pilot cross-over study. International Journal of Food Sciences and Nutrition, 67(1), 47-52.
  • Li, H. Y., Hao, Z. B., Wang, X. L., Huang, L. & Li, J.P. (2009). Antioxidant activities of extracts and fractions from Lysimachia foenum-graecum Hance. Bioresource Technology, 100(2), 970-974.
  • Masondo, N. A., Stafford, G. I., Aremu, A.O. & Makunga, N.P. (2019). Acetylcholinesterase inhibitors from southern African plants: An overview of ethnobotanical, pharmacological potential and phytochemical research including and beyond Alzheimer's disease treatment. South African Journal of Botany, 120, 39-64.
  • McCune, L.M., Kubota, C., Stendell-Hollis, N.R. & Thomson, C.A. (2010). Cherries and health: a review. Critical Reviews in Food Science and Nutrition, 51(1), 1-12.
  • Mollica, A., Zengin, G., Durdagi, S., Ekhteiari Salmas, R., Macedonio, G., Stefanucci, A., Dimmito, M.P. & Novellino, E. (2019). Combinatorial peptide library screening for discovery of diverse α-glucosidase inhibitors using molecular dynamics simulations and binary QSAR models. Journal of Biomolecular Structure and Dynamics, 37(3), 726-740.
  • Mollica, A., Zengin, G., Stefanucci, A., Ferrante, C., Menghini, L., Orlando, G., Brunetti, L., Locatelli, M., Dimmito, M.P., Novellino, E., Wakeel, O.K., Ogundeji, M.O., Onaolapo, A.Y. & Onaolapo, O.J. (2018). Nutraceutical potential of Corylus avellana daily supplements for obesity and related dysmetabolism. Journal of Functional Foods, 47, 562-574.
  • Murayyan, A.I., Manohar, C.M., Hayward, G. & Neethirajan, S. (2017). Antiproliferative activity of Ontario grown onions against colorectal adenocarcinoma cells. Food Research International, 96, 12-18.
  • Nawirska-Olszańska, A., Kolniak-Ostek, J., Oziembłowski, M., Ticha, A., Hyšpler, R., Zadak, Z., Zidova, P. & Paprstein, F. (2017). Comparison of old cherry cultivars grown in Czech Republic by chemical composition and bioactive compounds. Food Chemistry, 228(1), 136-142.
  • Rauf, A. & Jehan, N. (2017). Natural products as a potential enzyme inhibitors from medicinal plants. In Enzyme Inhibitors and Activators, 165-177.
  • Scalbert, A., Johnson, I.T. & Saltmarsh, M. (2005). Polyphenols: antioxidants and beyond. The American Journal of Clinical Nutrition, 81(1), 215-217.
  • Serradilla, M.J., Martín, A., Ruiz-Moyano, S., Hernández, A., López-Corrales, M. & de Guía Córdoba, M. (2012). Physicochemical and sensorial characterisation of four sweet cherry cultivars grown in Jerte Valley (Spain). Food Chemistry, 133(4), 1551-1559.
  • Średnicka-Tober, D., Ponder, A., Hallmann, E., Głowacka, A. & Rozpara, E. (2019). The profile and content of polyphenols and carotenoids in local and commercial sweet cherry fruits (Prunus avium L.) and their antioxidant activity in vitro. Antioxidants, 8(11), 534.
  • Usenik, V., Fabčič, J. & Štampar, F. (2008). Sugars, organic acids, phenolic composition and antioxidant activity of sweet cherry (Prunus avium L.). Food Chemistry, 107(1), 185-192.
  • Uysal, A., Ozer, O.Y., Zengin, G., Stefanucci, A., Mollica, A., Picot-Allain, C. M. N. & Mahomoodally, M. F. (2019). Multifunctional approaches to provide potential pharmacophores for the pharmacy shelf: Heracleum sphondylium L. subsp. ternatum (Velen.) Brummitt. Computational Biology and Chemistry, 78, 64-73.
  • Uysal, S., Zengin, G., Locatelli, M., Bahadori, M. B., Mocan, A., Bellagamba, G., De Luca, E., Mollica, A. & Aktumsek, A. (2017). Cytotoxic and enzyme inhibitory potential of two Potentilla species (P. speciosa L. and P. reptans Willd.) and their chemical composition. Frontiers in Pharmacology, 8, 290.
  • Uysal, S. & Aktumsek, A. (2015). A phytochemical study on Potentilla anatolica: an endemic Turkish plant. Industrial Crops and Products, 76, 1001-1007.
  • Wu, T., Tang, Q., Yu, Z., Gao, Z., Hu, H., Chen, W., Zheng, X. & Yu, T. (2014). Inhibitory effects of sweet cherry anthocyanins on the obesity development in C57BL/6 mice. International Journal of Food Sciences and Nutrition, 65(3), 351-359.
  • Zengin, G., Stefanucci, A., Rodrigues, M.J., Mollica, A., Custodio, L., Aumeeruddy, M.Z., & Mahomoodally, M. F. (2019). Scrophularia lucida L. as a valuable source of bioactive compounds for pharmaceutical applications: in vitro antioxidant, anti-inflammatory, enzyme inhibitory properties, in silico studies, and HPLC profiles. Journal of Pharmaceutical and Biomedical Analysis, 162, 225-233.
There are 30 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Sengul Uysal This is me

Publication Date December 30, 2020
Published in Issue Year 2020 Volume: 20 Issue: 3

Cite

APA Uysal, S. (2020). Comparative Antioxidant Capacity and Enzyme Inhibitory Effect of Extracts from Prunus avium Leaves. Kastamonu University Journal of Forestry Faculty, 20(3), 234-242. https://doi.org/10.17475/kastorman.849538
AMA Uysal S. Comparative Antioxidant Capacity and Enzyme Inhibitory Effect of Extracts from Prunus avium Leaves. Kastamonu University Journal of Forestry Faculty. December 2020;20(3):234-242. doi:10.17475/kastorman.849538
Chicago Uysal, Sengul. “Comparative Antioxidant Capacity and Enzyme Inhibitory Effect of Extracts from Prunus Avium Leaves”. Kastamonu University Journal of Forestry Faculty 20, no. 3 (December 2020): 234-42. https://doi.org/10.17475/kastorman.849538.
EndNote Uysal S (December 1, 2020) Comparative Antioxidant Capacity and Enzyme Inhibitory Effect of Extracts from Prunus avium Leaves. Kastamonu University Journal of Forestry Faculty 20 3 234–242.
IEEE S. Uysal, “Comparative Antioxidant Capacity and Enzyme Inhibitory Effect of Extracts from Prunus avium Leaves”, Kastamonu University Journal of Forestry Faculty, vol. 20, no. 3, pp. 234–242, 2020, doi: 10.17475/kastorman.849538.
ISNAD Uysal, Sengul. “Comparative Antioxidant Capacity and Enzyme Inhibitory Effect of Extracts from Prunus Avium Leaves”. Kastamonu University Journal of Forestry Faculty 20/3 (December 2020), 234-242. https://doi.org/10.17475/kastorman.849538.
JAMA Uysal S. Comparative Antioxidant Capacity and Enzyme Inhibitory Effect of Extracts from Prunus avium Leaves. Kastamonu University Journal of Forestry Faculty. 2020;20:234–242.
MLA Uysal, Sengul. “Comparative Antioxidant Capacity and Enzyme Inhibitory Effect of Extracts from Prunus Avium Leaves”. Kastamonu University Journal of Forestry Faculty, vol. 20, no. 3, 2020, pp. 234-42, doi:10.17475/kastorman.849538.
Vancouver Uysal S. Comparative Antioxidant Capacity and Enzyme Inhibitory Effect of Extracts from Prunus avium Leaves. Kastamonu University Journal of Forestry Faculty. 2020;20(3):234-42.

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