Yıldız Gölü Sedimentinden İzole Edilen Aktinobakterilerin Antimikrobiyal ve Enzim Üretim Kapasitelerinin Araştırılması

Year 2019, Volume: 9 Issue: 1, 144 - 151, 30.06.2019

Kadriye Özcan

https://doi.org/10.31466/kfbd.563428

Cited By: 2

Abstract

Bu çalışmada, Yıldız Gölü (Gümüşhane)
sedimentinden aktinobakteri izolasyonu gerçekleştirilmiş ve izolatlar
antimikrobiyal aktivite ve enzim üretme kapasiteleri (amilaz, lipaz, proteaz,
pektinaz, selülaz) bakımından incelenmiştir. Tarama, uygun besiyeriler içeren
petrilerde gerçekleştirilmiştir. İzolasyon için SCA besiyeri kullanılmış ve
besiyeriye nistatin ve nalidiksik asit ilave edilmiştir. Antimikrobiyal
aktivite tespiti için, çapraz çizgi ekim yöntemi ve C. tropicalis, C. albicans, E. coli, P. aeruginosa, MRSA, E. faecium, E. fecalis standart suşları tercih edilmiştir. Sonuç olarak izolatların  %55’i
en az bir test mikroorganizmasına karşı antimikrobiyal aktivite gösterirken
%66.6’sının ise en az bir enzimi üretebildiği belirlenmiştir. Bununla birlikte
izolatların %22.2’si ne antimikrobiyal aktivite ne de enzim üretme yeteneğine
sahip bulunmuştur.  Bu çalışma Yıldız
Gölü’nden aktinobakteri izolasyonu ve izolatların biyolojik aktivitesi üzerine
gerçekleştirilen ilk araştırmadır. Sonuç olarak, elde edilen aktinobakterilerin
endüstriyel ve farmakolojik çalışmalar için kaynak olabileceği öngörülmekle birlikte
aktif bileşiklerin izolasyonu ve karakterizasyonu için ileri çalışmalara
ihtiyaç duyulmaktadır

Keywords

Antimikrobiyal aktivite, Enzim üretimi, İzolasyon, Tarama

Investigation of Antimicrobial and Enzyme Production Capacities of Actinobacteria Isolated from Yıldız Lake Sediment

Abstract

In this study, actinobacteria was isolated from
Yıldız Lake (Gümüşhane) sediment and the isolates were examined for
antimicrobial activity and enzyme production capacities (amylase, lipase,
protease, pectinase, cellulase). Screening was carried out on petri dishes
containing suitable media. SCA medium was used for isolation and nistatin and
nalidixic acid were added. For the detection of antimicrobial activity
cross-streak method and C. tropicalis, C.
albicans, E. coli, P. aeruginosa, MRSA, E. faecium, E. fecalis standard
strains were used. As a result, 55% of the isolates showed antimicrobial
activity agains to at least one test microorganism while 66.6% were able to
produce at least one enzyme. However, 22.2% of the isolates were not capable of
producing either antimicrobial activity or enzyme. This study was the first study
on isolation of actinobacteria from Yıldız Lake and the biological activity of
isolates. As a results, it is expected that the actinobacteria obtained from
this sediment may be the source for industrial and pharmacological studies, but
further studies are needed for the isolation and characterization of the active
compounds.

Keywords

Antimicrobial activity, Enzyme production, Isolation, Screening

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