Year 2019, Volume 9 , Issue 1, Pages 176 - 185 2019-06-30

Alkanna Tinctoria (L.) Tausch Kök Ekstraktlarının Antimikrobiyal Aktivitesi ve Sitotoksisitesi
Antimicrobial Activity and Cytotoxicity of Alkanna Tinctoria (L.) Tausch Root Extracts

Hatice Aysun MERCİMEK TAKCI [1] , Filiz UCAN TURKMEN [2] , Fatma Ceren ANLAS [3] , Fulya USTUN ALKAN [4] , Pemra BAKIRHAN [5] , Cihan DEMİR [6] , Nazım SEKEROGLU [7]


DNA-topoizomerazları olarak bilinen naftokinonları üreten Alkanna tinctoria (L.) Tausch, Türkiye’de özellikle yara iyileştirmede geleneksel terapötik ajan olarak kullanılmaktadır. Alkanna tinctoria metanol, etanol ve asetonitril kök ekstraktlarının 9 klinik mikroorganizmaya karşı antimikrobiyal aktivitesi ve köpek meme kanser hücre hattı (CMT-U27) üzerinde sitotoksititesi MTT analizi kullanılarak araştırılmıştır. Alkanat kök ekstraktları 9 mikroorganizmadan sadece ikisine (Proteus spp. and S. haemolyticus) karşı antimikrobiayl aktivite göstermiştir. CMT-U27 kanser hücrelerine yönelik in vitro çalışmada, 100 µg/mL konsantrasyondaki asetonitril kök ekstraktı güçlü ve önemli bir hücre büyümesini engelleyici etki göstermiştir. Sonuçlarımız Alkanat asetonitril kök ekstraktının biyoaktif metabolitlerinin tanımlanmasını takiben potansiyel terapötik ajan olarak değerlendirilebileceğini açıkça ifade etmektedir.

Alkanna tinctoria (L.) Tausch produced naphthoquinones known as DNA-topoisomerases inhibitors has been used as the traditional therapeutic agent, especially wound healing in Turkey. The antimicrobial activity against 9 clinical microorganisms and cytotoxicity on canine mammary carcinoma cell line (CMT-U27) of Alkanna tinctoria root extracts (methanol, ethanol and acetonitrile) were researched by using MTT analysis. Alkanet root extracts showed antimicrobial activity against 2 (Proteus spp. and S. haemolyticus) out of 9 microorganisms. In vitro study towards CMT-U27 cancer cells, acetonitrile root extract at 100 µg/mL concentration showed strong and significant anti-proliferative effect. Our results are manifested that the Alkanet acetonitrile root extract can be evaluated as potential therapeutic agent, immediately after identification of bio-active metabolits in extract.

  • Refernas 1 Akcin, O. E., Kandemur, N., and Cansaran, A., (2004). Morphological and Anatomical Study on Endemic Alkanna haussknechtii Bornm. (Boraginaceae), Critically Endangered in Turkey. Turkish Journal of Botany, 28, 591-598.
  • Refernas 2 Alwahibi, M. S., and Perveen, K., (2017). Chemical analysis by GC-MS and in vitro antibacterial activity of Alkanna tinctoria extracts against skin infection causing bacteria. Biomedical Research, 28(18), 7946-7949.
  • Refernas 3 Amal, M. E., and Abdel-Hamid, A. M. E., (2011). DNA Fingerprint of Alkanna tinctoria Subspecies in Misurata, Libya. Middle-East Journal of Scientific Research, 7(4), 555-560.
  • Refernas 4 Assimopoulou, A. N., Boskou, D., and Papageorgiou, V. P., (2005). Antioxidant activities of alkannin, shikonin and Alkanna tinctoria root extracts in oil substrates. Food Chemistry, 87, 433-438.
  • Refernas 5 Elsharkawy, E., Elshathely, M., Jaleel, G. A., and Al-Johar, H. I., (2013). Anti-inflammatory effects of medicinal plants mixture used by Bedouin people in Saudi Arabia. De Gruyter Open, 59(3), 76-87.
  • Refernas 6 Gumus, K., Ozlu, Z, K., (2007). The effect of a beeswax, olive oil and Alkanna tinctoria (L.) Tausch mixture in burn injuries: An experimental study with a control group. Complementary Therapies in Medicine, 34, 66-73.
  • Refernas 7 Hayta, S., Polat, R., and Selvi, S., (2014). Traditional uses of medicinal plants in Elazıg Turkey. Journal of Ethnopharmacology, 154, 613-623.
  • Refernas 8 Kayabası, N., Sanlı, H. S., and Etikan, S., (2000). Havaciva (Alkanna tinctoria (L.) Tausch) ve Labada (Rumex conglomeratus Murr.) Bitkilerinden Elde Edilen Renkler ve Bu Renklerin Isık ve Surtunme Haslıkları Uzerinde Bir Arastırma. Journal of Agricultural Science, 10(1):7-10.
  • Refernas 9 Khan, U. A., Rahman, H., Qasim, M., Hussain, A., Azizllah, A., Murad, W., Khan, Z., Anees, M., and Adnan, M., (2015). Alkanna tinctoria leaves extracts: a prospective remedy against multidrug resistant human pathogenic bacteria. BMC Complementary and Alternative Medicine, 15(127), 1-6.
  • Refernas 10 Kheiri, A., Amini, S., Javidan, A. N., Saghafi, M. M., and Khorasani, G., (2017). The effects of Alkanna tinctoria Tausch on split-thickness skin graft donor site management: a randomized, blinded placebo-controlled trial. BMC Complementary and Alternative Medicine, 17, 1-5.
  • Refernas 11 Mohammed, M. S, Ali, A. A. M., Basudan, O., El Tahir, K. E. H., Osman, B., and Wadah, J. A. O., (2016). Evaluation of antipyretic, antinocieptive and sedative effects of Tribulus terrestris, Mimosa pigra and Alkanna tinctoria methanolic extracts. The Journal of Phytopharmacology. 5(3), 105-107.
  • Refernas 12 Ozer, M. S., Sarikurkcu, C., Tepe, B., and Cam, S., (2010). Essential Oil Composition and Antioxidant Activities of Alkanet (Alkanna tinctoria subsp. tinctoria). Food Science and Biotechnology, 19(5), 1177-1183.
  • Refernas 13 Plyta, Z. F., Li, T., Papageorgiou, V. P., Mellidis, A. S., Assimopoulou, A. N., Pitsinos, E. N., and Couladouros, E. A., (1998). Inhibition of Topoisomerase I by Naphthoquinone Derivatives. Bioorganic & Medicinal Chemistry Letters, 8, 3385-3390.
  • Refernas 14 Sengul, M., Yildiz, H., Gungor, N., Cetin, B., Eser, Z., and Ercisli, S., (2009). Total Phenolic Content, Antioxidant And Antimicrobial Activities of Some Medicinal Plants. Pakistan Journal of Pharmaceutical Sciences, 22(1), 102-106.
  • Refernas 15 Sevimli-Gur, C., Akgun, I. H., Deliloglu-Gurhan, I., Korkmaz, K. S., and Bedir, E., (2010). Cytotoxic Naphthoquinones from Alkanna cappadocica. Journal of Natural Product, 73, 860-864.
  • Refernas 16 Tsermentseli, S. K., Manesiotis, P., Assimopoulou, A. N., and Papageorgiou, V. P., (2013). Molecularly imprinted polymers for the isolation of bioactive naphthoquinones from plant extracts. Journal of Chromatography A, 1315, 15-20.
  • Refernas 17 Tung, N. H., Du, G-J., Wang, C-Z., Yuan, C-S., and Shoyama, Y., (2013). Naphthoquinone components from Alkanna tinctoria (L.) Tausch showed significant antiproliferative effects on human colorectal cancer cells. Phytotherapy Research, 27(1), 66-70.
  • Refernas 18 Ustun Alkan, F., Anlas, C., Ustuner, O., Bakirel, T., and Sarı, A. B., (2014). Antioxidant and Proliferative Effects of Aqueous and Ethanolic Extracts of Symphytum officinale on 3T3-Swiss Albino Cells Fibroblast Cell Line. Asian Journal of Plant Science & Research, 4(4), 62-68.
  • Refernas 19 Yazdinezhad, A., Monsef-Esfahani, M., and Ghahremani, M. H., (2013). Effect of Alkanna frigid Extracts on 3t3 Fibroblast Cell Proliferation. International Journal of Pharma and Bio Sciences, 3(4), 212-215.
Primary Language en
Subjects Basic Sciences
Journal Section Articles
Authors

Orcid: 0000-0002-5394-4959
Author: Hatice Aysun MERCİMEK TAKCI (Primary Author)
Institution: Kilis 7 Arlık Üniversitesi
Country: Turkey


Orcid: 0000-0002-3653-9433
Author: Filiz UCAN TURKMEN
Institution: Kilis 7 Aralık Üniversitesi

Orcid: 0000-0002-7997-016X
Author: Fatma Ceren ANLAS

Orcid: 0000-0003-1782-9467
Author: Fulya USTUN ALKAN

Orcid: 0000-0003-4172-0648
Author: Pemra BAKIRHAN

Orcid: 0000-0003-4835-2892
Author: Cihan DEMİR

Orcid: 0000-0002-0630-0106
Author: Nazım SEKEROGLU

Dates

Publication Date : June 30, 2019

Bibtex @research article { kfbd566276, journal = {Karadeniz Fen Bilimleri Dergisi}, issn = {1309-4726}, eissn = {2564-7377}, address = {Giresun Üniversitesi Fen Bilimleri Enstitüsü Güre Yerleşkesi 28200 / Giresun}, publisher = {Giresun University}, year = {2019}, volume = {9}, pages = {176 - 185}, doi = {10.31466/kfbd.566276}, title = {Antimicrobial Activity and Cytotoxicity of Alkanna Tinctoria (L.) Tausch Root Extracts}, key = {cite}, author = {MERCİMEK TAKCI, Hatice Aysun and TURKMEN, Filiz UCAN and ANLAS, Fatma Ceren and USTUN ALKAN, Fulya and BAKIRHAN, Pemra and DEMİR, Cihan and SEKEROGLU, Nazım} }
APA MERCİMEK TAKCI, H , TURKMEN, F , ANLAS, F , USTUN ALKAN, F , BAKIRHAN, P , DEMİR, C , SEKEROGLU, N . (2019). Antimicrobial Activity and Cytotoxicity of Alkanna Tinctoria (L.) Tausch Root Extracts. Karadeniz Fen Bilimleri Dergisi , 9 (1) , 176-185 . DOI: 10.31466/kfbd.566276
MLA MERCİMEK TAKCI, H , TURKMEN, F , ANLAS, F , USTUN ALKAN, F , BAKIRHAN, P , DEMİR, C , SEKEROGLU, N . "Antimicrobial Activity and Cytotoxicity of Alkanna Tinctoria (L.) Tausch Root Extracts". Karadeniz Fen Bilimleri Dergisi 9 (2019 ): 176-185 <https://dergipark.org.tr/en/pub/kfbd/issue/45378/566276>
Chicago MERCİMEK TAKCI, H , TURKMEN, F , ANLAS, F , USTUN ALKAN, F , BAKIRHAN, P , DEMİR, C , SEKEROGLU, N . "Antimicrobial Activity and Cytotoxicity of Alkanna Tinctoria (L.) Tausch Root Extracts". Karadeniz Fen Bilimleri Dergisi 9 (2019 ): 176-185
RIS TY - JOUR T1 - Antimicrobial Activity and Cytotoxicity of Alkanna Tinctoria (L.) Tausch Root Extracts AU - Hatice Aysun MERCİMEK TAKCI , Filiz UCAN TURKMEN , Fatma Ceren ANLAS , Fulya USTUN ALKAN , Pemra BAKIRHAN , Cihan DEMİR , Nazım SEKEROGLU Y1 - 2019 PY - 2019 N1 - doi: 10.31466/kfbd.566276 DO - 10.31466/kfbd.566276 T2 - Karadeniz Fen Bilimleri Dergisi JF - Journal JO - JOR SP - 176 EP - 185 VL - 9 IS - 1 SN - 1309-4726-2564-7377 M3 - doi: 10.31466/kfbd.566276 UR - https://doi.org/10.31466/kfbd.566276 Y2 - 2019 ER -
EndNote %0 Karadeniz Fen Bilimleri Dergisi Antimicrobial Activity and Cytotoxicity of Alkanna Tinctoria (L.) Tausch Root Extracts %A Hatice Aysun MERCİMEK TAKCI , Filiz UCAN TURKMEN , Fatma Ceren ANLAS , Fulya USTUN ALKAN , Pemra BAKIRHAN , Cihan DEMİR , Nazım SEKEROGLU %T Antimicrobial Activity and Cytotoxicity of Alkanna Tinctoria (L.) Tausch Root Extracts %D 2019 %J Karadeniz Fen Bilimleri Dergisi %P 1309-4726-2564-7377 %V 9 %N 1 %R doi: 10.31466/kfbd.566276 %U 10.31466/kfbd.566276
ISNAD MERCİMEK TAKCI, Hatice Aysun , TURKMEN, Filiz UCAN , ANLAS, Fatma Ceren , USTUN ALKAN, Fulya , BAKIRHAN, Pemra , DEMİR, Cihan , SEKEROGLU, Nazım . "Antimicrobial Activity and Cytotoxicity of Alkanna Tinctoria (L.) Tausch Root Extracts". Karadeniz Fen Bilimleri Dergisi 9 / 1 (June 2019): 176-185 . https://doi.org/10.31466/kfbd.566276
AMA MERCİMEK TAKCI H , TURKMEN F , ANLAS F , USTUN ALKAN F , BAKIRHAN P , DEMİR C , SEKEROGLU N . Antimicrobial Activity and Cytotoxicity of Alkanna Tinctoria (L.) Tausch Root Extracts. KFBD. 2019; 9(1): 176-185.
Vancouver MERCİMEK TAKCI H , TURKMEN F , ANLAS F , USTUN ALKAN F , BAKIRHAN P , DEMİR C , SEKEROGLU N . Antimicrobial Activity and Cytotoxicity of Alkanna Tinctoria (L.) Tausch Root Extracts. Karadeniz Fen Bilimleri Dergisi. 2019; 9(1): 185-176.