Antimicrobial Activity and Cytotoxicity of Alkanna Tinctoria (L.) Tausch Root Extracts

Year 2019, Volume: 9 Issue: 1, 176 - 185, 30.06.2019

Hatice Aysun Mercimek Takcı , Filiz Ucan Turkmen Fatma Ceren Anlas Fulya Ustun Alkan , Pemra Bakırhan , Cihan Demir Nazım Sekeroglu

https://doi.org/10.31466/kfbd.566276

Cited By: 3

Abstract

Alkanna tinctoria (L.) Tausch produced naphthoquinones known as
DNA-topoisomerases inhibitors has been used as the traditional therapeutic agent, especially wound healing in
Turkey. The antimicrobial activity against 9 clinical microorganisms and
cytotoxicity on canine mammary carcinoma cell line (CMT-U27) of Alkanna tinctoria root extracts (methanol,
ethanol and acetonitrile) were researched by using MTT analysis. Alkanet root extracts showed antimicrobial activity
against 2 (Proteus spp. and S. haemolyticus)
out of 9 microorganisms. In vitro study towards CMT-U27 cancer cells, acetonitrile
root extract at 100 µg/mL concentration showed
strong and significant anti-proliferative effect. Our
results are manifested that the Alkanet acetonitrile root extract can be
evaluated as potential therapeutic agent, immediately after identification of bio-active metabolits in extract.

Keywords

Alkanet root, Alkanna tinctoria, antimicrobial, cytotoxicity

Alkanna Tinctoria (L.) Tausch Kök Ekstraktlarının Antimikrobiyal Aktivitesi ve Sitotoksisitesi

Abstract

DNA-topoizomerazları
olarak bilinen naftokinonları üreten Alkanna
tinctoria (L.) Tausch, Türkiye’de özellikle yara iyileştirmede geleneksel
terapötik ajan olarak kullanılmaktadır. Alkanna tinctoria metanol, etanol
ve asetonitril kök ekstraktlarının 9 klinik mikroorganizmaya karşı
antimikrobiyal aktivitesi ve köpek meme kanser hücre hattı (CMT-U27) üzerinde
sitotoksititesi MTT analizi kullanılarak araştırılmıştır. Alkanat kök
ekstraktları 9 mikroorganizmadan sadece ikisine (Proteus spp. and S. haemolyticus) karşı antimikrobiayl
aktivite göstermiştir. CMT-U27 kanser hücrelerine yönelik in vitro çalışmada, 100 µg/mL
konsantrasyondaki asetonitril kök ekstraktı güçlü ve önemli bir hücre
büyümesini engelleyici etki göstermiştir. Sonuçlarımız Alkanat asetonitril kök ekstraktının biyoaktif
metabolitlerinin tanımlanmasını takiben potansiyel terapötik ajan olarak
değerlendirilebileceğini açıkça ifade etmektedir.

Keywords

Alkanat kök, Alkanna tinctoria, antimikrobiyal, sitotoksisite

References

• Refernas 1 Akcin, O. E., Kandemur, N., and Cansaran, A., (2004). Morphological and Anatomical Study on Endemic Alkanna haussknechtii Bornm. (Boraginaceae), Critically Endangered in Turkey. Turkish Journal of Botany, 28, 591-598.
• Refernas 2 Alwahibi, M. S., and Perveen, K., (2017). Chemical analysis by GC-MS and in vitro antibacterial activity of Alkanna tinctoria extracts against skin infection causing bacteria. Biomedical Research, 28(18), 7946-7949.
• Refernas 3 Amal, M. E., and Abdel-Hamid, A. M. E., (2011). DNA Fingerprint of Alkanna tinctoria Subspecies in Misurata, Libya. Middle-East Journal of Scientific Research, 7(4), 555-560.
• Refernas 4 Assimopoulou, A. N., Boskou, D., and Papageorgiou, V. P., (2005). Antioxidant activities of alkannin, shikonin and Alkanna tinctoria root extracts in oil substrates. Food Chemistry, 87, 433-438.
• Refernas 5 Elsharkawy, E., Elshathely, M., Jaleel, G. A., and Al-Johar, H. I., (2013). Anti-inflammatory effects of medicinal plants mixture used by Bedouin people in Saudi Arabia. De Gruyter Open, 59(3), 76-87.
• Refernas 6 Gumus, K., Ozlu, Z, K., (2007). The effect of a beeswax, olive oil and Alkanna tinctoria (L.) Tausch mixture in burn injuries: An experimental study with a control group. Complementary Therapies in Medicine, 34, 66-73.
• Refernas 7 Hayta, S., Polat, R., and Selvi, S., (2014). Traditional uses of medicinal plants in Elazıg Turkey. Journal of Ethnopharmacology, 154, 613-623.
• Refernas 8 Kayabası, N., Sanlı, H. S., and Etikan, S., (2000). Havaciva (Alkanna tinctoria (L.) Tausch) ve Labada (Rumex conglomeratus Murr.) Bitkilerinden Elde Edilen Renkler ve Bu Renklerin Isık ve Surtunme Haslıkları Uzerinde Bir Arastırma. Journal of Agricultural Science, 10(1):7-10.
• Refernas 9 Khan, U. A., Rahman, H., Qasim, M., Hussain, A., Azizllah, A., Murad, W., Khan, Z., Anees, M., and Adnan, M., (2015). Alkanna tinctoria leaves extracts: a prospective remedy against multidrug resistant human pathogenic bacteria. BMC Complementary and Alternative Medicine, 15(127), 1-6.
• Refernas 10 Kheiri, A., Amini, S., Javidan, A. N., Saghafi, M. M., and Khorasani, G., (2017). The effects of Alkanna tinctoria Tausch on split-thickness skin graft donor site management: a randomized, blinded placebo-controlled trial. BMC Complementary and Alternative Medicine, 17, 1-5.
• Refernas 11 Mohammed, M. S, Ali, A. A. M., Basudan, O., El Tahir, K. E. H., Osman, B., and Wadah, J. A. O., (2016). Evaluation of antipyretic, antinocieptive and sedative effects of Tribulus terrestris, Mimosa pigra and Alkanna tinctoria methanolic extracts. The Journal of Phytopharmacology. 5(3), 105-107.
• Refernas 12 Ozer, M. S., Sarikurkcu, C., Tepe, B., and Cam, S., (2010). Essential Oil Composition and Antioxidant Activities of Alkanet (Alkanna tinctoria subsp. tinctoria). Food Science and Biotechnology, 19(5), 1177-1183.
• Refernas 13 Plyta, Z. F., Li, T., Papageorgiou, V. P., Mellidis, A. S., Assimopoulou, A. N., Pitsinos, E. N., and Couladouros, E. A., (1998). Inhibition of Topoisomerase I by Naphthoquinone Derivatives. Bioorganic & Medicinal Chemistry Letters, 8, 3385-3390.
• Refernas 14 Sengul, M., Yildiz, H., Gungor, N., Cetin, B., Eser, Z., and Ercisli, S., (2009). Total Phenolic Content, Antioxidant And Antimicrobial Activities of Some Medicinal Plants. Pakistan Journal of Pharmaceutical Sciences, 22(1), 102-106.
• Refernas 15 Sevimli-Gur, C., Akgun, I. H., Deliloglu-Gurhan, I., Korkmaz, K. S., and Bedir, E., (2010). Cytotoxic Naphthoquinones from Alkanna cappadocica. Journal of Natural Product, 73, 860-864.
• Refernas 16 Tsermentseli, S. K., Manesiotis, P., Assimopoulou, A. N., and Papageorgiou, V. P., (2013). Molecularly imprinted polymers for the isolation of bioactive naphthoquinones from plant extracts. Journal of Chromatography A, 1315, 15-20.
• Refernas 17 Tung, N. H., Du, G-J., Wang, C-Z., Yuan, C-S., and Shoyama, Y., (2013). Naphthoquinone components from Alkanna tinctoria (L.) Tausch showed significant antiproliferative effects on human colorectal cancer cells. Phytotherapy Research, 27(1), 66-70.
• Refernas 18 Ustun Alkan, F., Anlas, C., Ustuner, O., Bakirel, T., and Sarı, A. B., (2014). Antioxidant and Proliferative Effects of Aqueous and Ethanolic Extracts of Symphytum officinale on 3T3-Swiss Albino Cells Fibroblast Cell Line. Asian Journal of Plant Science & Research, 4(4), 62-68.
• Refernas 19 Yazdinezhad, A., Monsef-Esfahani, M., and Ghahremani, M. H., (2013). Effect of Alkanna frigid Extracts on 3t3 Fibroblast Cell Proliferation. International Journal of Pharma and Bio Sciences, 3(4), 212-215.