Adaçayının PEG ile İndüklenen Kuraklık Stresine Antioksidatif Yanıtları

Year 2022, Volume: 12 Issue: 1, 390 - 397, 15.06.2022

Özlem Arslan

https://doi.org/10.31466/kfbd.1080095

Abstract

Bu çalışma, polietilen glikol (PEG) ile indüklenen iki kuraklık uygulamasının (-0.4 ve -0.8 MPa) adaçayı (Salvia officinalis L.) bitkisi üzerindeki etkisini belirlemek amacıyla yapılmıştır. Yirmi beş günlük çelikler yedi gün boyunca kuraklık uygulamalarına maruz bırakılmıştır. Zar hasarı ve su kaybı, kuraklığın şiddeti ile kademeli olarak artmıştır. Zar bütünlüğündeki ve su içeriğindeki azalmalar adaçayının kuraklıktan etkilendiğini gösterirken, artan flavonoid ve antioksidan enzim aktiviteleri bile bu etkiyi azaltamamıştır. Su potansiyeli koşullarının azalmasıyla yaprakların H2O2 içeriğinin artması, kuraklığın neden olduğu oksidatif stresin göstergesidir. Yüksek seviyelerdeki SOD ve POD aktiviteleri, enzimlerin H2O2'nin uzaklaştırılmasında rol oynadığını göstermiştir. Adaçayı, kuraklığa dayanma toleransını başarıyla artırmıştır.

Keywords

Antioksidant enzimler, Salvia officinalis L., Su kıtlığı

Antioxidative Responses of Sage to PEG-induced Drought Stress

Abstract

The current study was conducted to find out the effect of two polyethylene glycol (PEG) induced drought treatments (-0.4 and -0.8 MPa) on sage (Salvia officinalis L.). Twenty five-day old cuttings were exposed to drought treatments for seven days. Membrane damage and water loss were gradually increased with the severity of drought. While reductions in membrane integrity and water content showed that sage was affected by drought, even increased flavonoid and antioxidant enzyme activities could not alleviate this effect. H2O2 content of leaves increased with decreasing water potential conditions, indication an oxidative stress caused by drought. Elevated levels of SOD and POD activities indicated that the enzymes were involved in scavenging of H2O2. Sage was successfully increased the tolerance to withstand to drought. 

Keywords

Antioxidant enzymes, Salvia officinalis L., Water deficit

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