Kirklareli University Journal of Engineering and Science

2458-7494 2458-7613

Kirklareli University

10.34186/klujes.1314299

Industrial Microbiology

Endüstriyel Mikrobiyoloji

Toksijenik Aspergillus flavus’un Büyümesini Kontrol Etmek için Thymus longicaulis’in Değerlendirilmesi

https://orcid.org/0000-0003-4091-2648

Balkan

Bilal

KIRKLARELİ ÜNİVERSİTESİ

https://orcid.org/0000-0001-7148-4792

Balkan

Seda

Kırklareli Üniversitesi

12 31 2023

9 2 443 453 06 14 2023 12 24 2023

2015

Kirklareli University Journal of Engineering and Science

Bu araştırmada Lamiaceae, familyalasına ait Thymus longicaulis’in sulu ekstraktlarının Aspergillus flavus’ un büyümesi üzerine olan etkisinin in vitro ve in vivo olarak değerlendirilmesi gerçekleştirilmiştir. Kurutulmuş T. longicaulis’in tozları A flavus’ un misel büyümesini %70.53 oranında inhibe etmiştir. T. longicaulis sulu ekstraktlarının A. flavus’a karşı minimum inhibitör konsantrasyon (MİK) değeri 1000 µg/mL idi. T. longicaulis sulu ekstraktları sıvı besiyerindeki 5 günlük saklanma sürelerinden sonra petri kaplarına aktarıldığında ayçiçeği, buğday, mısır ve pirinçte yapay olarak oluşturulan A. flavus çürümesini tamamen engelleyememiştir. Test edilen tüm konsantrasyonlarda 5 günlük inkübasyon süresi boyunca T. longicaulis sulu ekstraktlarının sadece ayçiçeğinde etkin olduğu belirlenmiştir (p<0.05). T. longicaulis sulu ekstraktlarının hifal morfoloji üzerine lizis, çökertme, yassılaşma ve kırışık hücre yüzeyli hücreler dejeneratif değişimlerini yaptığı SEM analizi ile tespit edilmiştir. Araştırmanın sonuçlarına göre T. longicaulis sulu ekstraktlarının aflatoksin B1 üreticisi olan toksijenik A. flavus’un ayçiçeğinde oluşturduğu enfeksiyonları önleyebilmek için koruyucu olarak rol oynayabileceğini ifade edebiliriz.

Aspergillus flavus Thymus longicaulis Antifungal Bitki ekstraktı SEM

Kırklareli Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

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