3-APTMS TAKVİYELİ KİTOSAN-POLİVİNİL ALKOL HARMAN FİLMLERİNİN FİZİKSEL VE ANTİBAKTERİYEL AKTİVİTELERİ

Year 2023, Volume: 26 Issue: 1, 154 - 165, 15.03.2023

Nedim Gürler Ferit Can Yazdıç

https://doi.org/10.17780/ksujes.1187738

Abstract

Bu çalışmada kitosan (KT) ve polivinil alkol (PA) karışımına %0, %0.5, %1 ve %2 oranlarında 3-(aminopropil) trimetoksi silan (3-APTMS) ilave edilerek dökme metoduyla filmler hazırlanmıştır. Hazırlanan filmlerin FTIR, SEM, su buharı geçirgenliği, çözünürlük, opaklık ve renk özellikleri incelenmiştir. KT/PA, KT/PA-0.5, KT/PA-1 ve KT/PA-2 filmlerinin su buharı geçirgenliği sırasıyla 3.82×10-6 g m-1 s-1 Pa-1, 3.14×10-6 g m-1 s-1 Pa-1, 2.08×10-6 g m-1 s-1 Pa-1 ve 1.59×10-6 g m-1 s-1 Pa-1 olarak gerçekleşti. Filmleri su içinde çözünürlüğü azaldı. Artan 3-APTMS içeriği ile filmler daha opak oldu ve UV ışık bariyer özellik gösterdi. Renk özellikleri ise a* değeri artarken b* değeri ise azalarak filmler sarımsı-yeşil oldu. Ayrıca filmlerin Gram negatif (E. coli ) ve gram pozitif (S. aureus subsp. aureus) bakterilere karşı antimikrobiyal aktiviteleri değerlendirildi. Elde edilen KT/PA tek başına S. aureus subsp. aureus (ATCC 25923) ve E. coli (ATCC 25922) ye karşı herhangi bir antimikrobiyal aktiviteyi göstermedi. Dikkat çeken nokta KT/PA'ya eklenen %2 3-APTMS antibakteriyel aktivitenin standart olarak kullanılan ampisilin ile aynı olmasıdır.

Keywords

Kitosan, polivinil alkol, antibakteriyel, modifikasyon, opaklık

PHYSICAL AND ANTIBACTERIAL ACTIVITIES OF 3-APTMS REINFORCED CHITOSAN-POLYVINYL ALCOHOL BLEND FILMS

Abstract

In this study, films were prepared by casting method by adding 3-(aminopropyl) trimethoxy silane (3-APTMS) at 0%, 0.5%, 1% and 2% ratios to the mixture of chitosan (KT) and polyvinyl alcohol (PA). FTIR, SEM, water vapour permeability, solubility, opacity, and colour properties of the prepared films were examined. The water vapor permeability of KT/PA, KT/PA-0.5, KT/PA-1 and KT/PA-2 films are 3.82×10-6 gm-1s-1Pa-1, 3.14×10-6 gm-1s-1Pa-1, 2.08×10-6 gm-1s-1Pa-1 and 1.59×10-6 gm-1s-1Pa-1, respectively. The films have decreased solubility in water. With increasing 3-APTMS content, the films became more opaque and showed UV light barrier properties. On the other hand, the a* value increased and the b* value decreased, making the films yellowish-green. In addition, the antimicrobial activities of the films against Gram-negative (E. coli ) and gram-positive (S. aureus subsp. aureus) bacteria were evaluated. KT/PA alone did not show any antimicrobial activity against S. aureus subsp. aureus (ATCC 25923) and E. coli (ATCC 25922). The remarkable point is that the 2% 3-APTMS antibacterial activity added to KT/PA is the same as that of standard ampicillin.

Keywords

chitosan, polyvinyl alchol, antibacterial, modification, opacity

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