ksu j. agric nat.

Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi

2619-9149

Kahramanmaras Sutcu Imam University

10.18016/ksutarimdoga.vi.826692

Agricultural, Veterinary and Food Sciences

Ziraat, Veterinerlik ve Gıda Bilimleri

Determination of Virus Resistance in Some Melon Accession Collected from Van Lake Basin by Mechanic Inoculation and Molecular Methods

Van Gölü Havzası’ndan Toplanan Bazı Kavun Genotiplerinde Virüse Karşı Dayanıklılığın Mekanik İnokulasyon ve Moleküler Yöntemlerle Belirlenmesi

https://orcid.org/0000-0003-1233-0077

Turan

Sibel

ERCİYES ÜNİVERSİTESİ

https://orcid.org/0000-0003-1208-032X

Erdinç

Çeknas

Van Yüzüncü Yıl Üniversitesi

08 31 2022

25 4 735 744 11 16 2020 09 20 2021

1997

Journal Of Agriculture and Nature

Melon (Cucumis melo L.) is an important vegetable crop grown in different geographical regions of Turkey. Zucchini yellow mosaic virus (ZYMV), cucumber mosaic virus (CMV) and watermelon mosaic virus (WMV) cause major economic losses because of their destructive effects on melon. In this study, 22 different melon genotypes collected from the Lake Van Basin, three resistant genotypes and two commercial varieties were screened against these three viruses by mechanical inoculation and molecular methods. In the first step, virus inoculation was performed on melon genotypes using ZYMV virus, and disease severity was determined according to 0-5 scale by performing morphological observations in the genotypes. In second stage of the study the presence of markers related to resistance genes against to ZYMV, CMV and WMV were determined by screening with 7 primers including 3 SCAR primers and 4 CAPS primers in these genotypes. None of the genotypes tested mechanically against the severe ZYMV isolate were found to be tolerant, resistant or immune to the virus. It was determined that the disease severity was ranged between 8.80-95.00% in melon genotypes. While bands were obtained from SCOPE14541 primer in 21 genotypes, only U6, U13 and YYU6 genotypes from 25 genotypes gave bands with SCAPB051046 primer that was developed based on the Creb-2 resistance gene. VirSq-F19 primer that was developed based on the resistance genes to ZYMV and WMV gave bands in all genotypes excluding YYU47. Only two of the 4 CAPS primers associated with the Zym resistance gene produced band.

Kavun (Cucumis melo L.) Türkiye ‘nin farklı coğrafik bölgelerinde yetiştirilen önemli bir sebze türüdür. Zucchini yellow mosaic virüs (ZYMV) başta olmak üzere cucumber mosaic virüs (CMV) ve watermelon mosaic virüs (WMV) kavunda oluşturduğu yıkıcı hasarlardan dolayı büyük ekonomik kayıplara neden olmaktadır. Bu çalışmada, Van Gölü Havzası’ndan toplanmış olan 22 farklı kavun genotipi ile birlikte 3 dayanıklı genotip ve 2 ticari çeşit bu üç virüse karşı mekanik inokülasyon ve moleküler yöntemlerle taranmıştır. Çalışmada ilk etapta ZYMV virüsü kullanılarak kavun genotiplerine virüs inokulasyonu gerçekleştirilmiş ve genotiplerde morfolojik gözlemler yapılarak 0-5 skalasına göre hastalık şiddeti belirlenmiştir. Çalışmanın ikinci aşamasında bu genotiplerde 3 adet SCAR ve 4 adet CAPS olmak üzere 7 adet primer ile tarama yapılarak ZYMV, CMV ve WMV’ ye karşı dayanıklılık ile ilgili belirteçlerin varlığı tespit edilmeye çalışılmıştır. Şiddetli ZYMV izolatına karşı mekanik olarak test edilen genotiplerin hiçbirinin virüse tolerant, dayanıklı ya da immun olmadığı saptanmıştır. Kavun genotiplerinde görülen hastalık şiddetinin %8.80-95.00 arasında değiştiği belirlenmiştir. SCOPE14541 primerinde 21 genotipte bant tespit edilirken, Creb-2 dayanıklılık genine bağlı olarak geliştirilen SCAPB051046 primerinde 25 genotipten yalnızca U6, U13 ve YYU6 genotiplerinde bant varlığı belirlenmiştir. ZYMV ve WMV’ ye karşı dayanıklılık genlerine bağlı olarak geliştirilmiş olan VirSq-F19 primeri YYU47 genotipi dışında tüm genotiplerde bant vermiştir. Zym dayanıklılık geni ile ilişkili 4 CAPS primerinden sadece ikisinden sonuç elde edilmiştir.

CAPS Kavun Mekanik inokülasyon SCAR Virüse dayanıklılık

CAPS Melon Mechanic inoculation SCAR Resistance to virus

Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırmalar Proje Koordinatörlüğü, Öğretim Üyesi Yerleştirme Programı (ÖYP)

FYL-2017-5899

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