Interaction of Three Populations of Pratylenchus thornei with Fusarium culmorum in Wheat Plant

Year 2022, Volume: 25 Issue: 5, 1042 - 1050, 31.10.2022

Fatma Gül Göze Özdemir Şerife Evrim Arıcı İbrahim Halil Elekcioğlu

https://doi.org/10.18016/ksutarimdoga.vi.947535

Cited By: 1

Abstract

The interaction of three populations of Pratylenchus thornei (SK11, SK24 and YLVC24) and Fusarium culmorum B4 isolate on İkizce wheat variety were investigated under controlled conditions with simultaneous and sequential inoculations. In the study, 1000 larva+adult inoculum density was used for nematode populations, while 50% diluted culture filtrate concentration was used in the fungal inoculum and 5 mL was applied to each pot. After about seven weeks, the wheat plants were removed and evaluated in terms of disease severity, final nematode density in soil and root, and nematode reproduction rate. It has been determined that the pathogenicity of the nematode populations is important in nematode and fungi interactions in the study. No interaction could be detected between the fungi and YLVC24, which has the lowest reproduction rate (6.6±0.1) among the populations, on wheat. In the SK24 population with the highest reproduction rate (10.4±0.4), the final nematode density was found to increase in simultaneous nematode and fungi application (12240.0±446.8), and fungi inoculation two weeks after nematode application (11672.0±389.3) compared to only nematode application (10436.0±446.8). When fungi was inoculated two weeks before nematode application on wheat, the population densities of SK11 and SK24 decreased. No difference was found between nematode populations in disease severity caused by fungi on wheat in simultaneous and sequential inoculations. It was determined that fungi application time was effective on total nematode density and reproduction rate of SK11 and SK24 populations. In the study, synergistic interaction was found only between the P. thornei SK24 population and F. culmorum in wheat.

Keywords

Wheat, Interaction, Fusarium culmorum, Fusarium culmorum, Fusarium culmorum, Fusarium culmorum, Interaction, Population density

Supporting Institution

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Project Number

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Buğday Bitkisinde Üç Pratylenchus thornei Popülasyonunun Fusarium culmorum ile Interaksiyonu

Abstract

İkizce buğday çeşidinde üç Pratylenchus thornei popülasyonu (SK11, SK24 ve YLVC24) ile Fusarium culmorum B4 izolatı'nın interaksiyonu eş zamanlı ve ardışık inokulasyonlarla kontrollü koşullarda araştırılmıştır. Çalışmada nematod popülasyonları için 1000 larva+ergin birey inokulum yoğunluğu kullanılırken, fungus inokulumunda %50 oranında seyreltilmiş kültür filtratı kullanılmış ve her saksıya 5 mL uygulanmıştır. Bitkiler yaklaşık yedi hafta sonra sökülerek değerlendirme işlemi hastalık şiddeti, toprak ve kökteki toplam nematod yoğunluğu ve üreme oranı açısından gerçekleştirilmiştir. Çalışmada nematod ve fungus etkileşimlerinde nematod popülasyonlarının patojenitesinin önemli olduğu belirlenmiştir. Popülasyonlar arasında üreme oranı en düşük olan YLVC24 (6.6±0.1) ile fungus arasında buğdayda bir interaksiyon saptanamamıştır. En yüksek üreme oranına sahip SK24 popülasyonunda (10.4±0.4) sadece nematod uygulaması (10436.0±446.8) ile karşılaştırıldığında eş zamanlı nematod ve fungus uygulaması (12240.0±446.8) ile nematod inokulasyonundan iki hafta sonra fungus uygulamasında (11672.0±389.3) toplam nematod yoğunluğunun arttığı tespit edilmiştir. Buğdayda nematod uygulamasından iki hafta önce fungus enfeksiyonu gerçekleştiğinde ise SK11 ve SK24 popülasyonlarının yoğunluklarında azalma görülmüştür. Buğdayda fungusun oluşturduğu hastalık şiddetinde eş zamanlı ve ardışık inokulasyonlarda nematod popülasyonları arasında farklılık bulunamamıştır. Fungus uygulama zamanının SK11 ve SK24 popülasyonlarının toplam nematod yoğunluğu ve üreme oranı üzerinde etkili olduğu belirlenmiştir. Çalışmada buğdayda sadece P. thornei SK24 popülasyonu ile F. culmorum arasında sinerjistik etkileşim bulunmuştur.

Keywords

Buğday, Fusarium culmorum, Interaksiyon, Popülasyon yoğunluğu, Pratylenchus thornei

Project Number

x

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