Smyrnium connatum Boiss. and Kotschy. Metanol Ekstresinin Biyolojik Aktivitesi

Year 2022, Volume: 25 Issue: 6, 1216 - 1224, 30.12.2022

Fatma Ayaz Nuraniye Eruygur Tuğsen Doğru Yavuz Bağcı Süleyman Doğu

https://doi.org/10.18016/ksutarimdoga.vi.997780

Cited By: 1

Abstract

Smyrnium connatum Boiss. and Kotschy bitkisi Türkiye'de "yabani kereviz" olarak bilinir ve bitkinin kökleri astım tedavisinde kullanılır. Farklı bölgelerde Smyrnium türleri insanlar tarafından diüretik, depuratif ve laksatif amaçlarla kullanılmaktadır. Bu çalışmanın amacı, Soxhlet ekstraktörü, ultrasonik destekli ekstraksiyon ve maserasyon yöntemleri kullanılarak S. connatum toprak üstü ve köklerinden hazırlanan metanol ekstrelerinin biyolojik aktivitelerini değerlendirmektir. S. connatum toprak üstü ve kök ekstreleri, çeşitli konsantrasyonlarda asetilkolinesteraz, bütirilkolinesteraz ve tirozinaz inhibitör aktiviteleri, radikal temizleyici potansiyelleri ve demir şelatlama kapasiteleri bakımından mikroplaka okuyucu kullanılarak test edildi. Yapılan araştırmalarda, farklı ekstraksiyon yöntemleri kullanılarak hazırlanan bitki ekstreleri arasında hem enzim inhibisyonu hem de antioksidan aktiviteleri önemli ölçüde farklı bulunmuştur. S. connatum toprak üstü kısımlarının antioksidan aktivitesi bakımından genel olarak daha yüksek tespit edilmiştir. S. connatum toprak üstü kısımları ve kökleri, her iki kolinesteraza karşı da yüksek inhibitör aktivite göstermiştir. Tüm ekstreler, tirozinaza karşı orta düzeyde inhibitör aktivite göstermiştir. Genel olarak S. connatum'un toprak üstü kısımlarının biyolojik aktivitesi köklerine göre daha aktif bulundu. Enzim inhibitörü ve antioksidan kapasite tayinlerinin sonucunda bitkinin nörodejeneratif hastalıkların tedavisinde kayda değer sonuçlar verebilecek potansiyellere sahip olduğu düşünülmektedir.

Keywords

Smynrium connatum, Enzim inhibisyonu, Antioksidan aktivite, Ekstraksiyon

Biological Activities of the Methanol Extracts of Smyrnium connatum Boiss. and Kotschy.

Abstract

Smyrnium connatum Boiss. and Kotschy is known as “yabani kereviz” in Turkey and its roots are used to treat asthma. Smyrnium species are used by people in different regions as diuretic, depurative and laxative. The aim of this analysis was to test the biological activities of methanol extracts obtained from aerial parts and roots of S. connatum using a Soxhlet extractor, ultrasonic assisted extraction, and maceration procedures. The extracts of S. connatum aerial parts and roots were tested for their acetylcholinesterase, butrylcholinesterase, and tyrosinase inhibitory activities, radical scavenging activity, and iron chelating activities at various concentration using microplate reader. In this study, significant differences were found in acetylcholinesterase, and butrylcholinesterase inhibition and antioxidant determinations between plant extracts prepared using different extraction methods. S. connatum aerial parts generally was detected to be more active in terms of antioxidant activity assay. S. connatum aerial parts and roots showed high inhibition activity against both cholinesterase enzymes. All extracts showed moderate inhibitory activity against tyrosinase. The biological activity of the aerial parts of S. connatum was generally found to be more active than roots of S. connatum. It was assumed that the determination of enzyme inhibitor and antioxidant capacity of the plant had remarkable potentials on the treatment of neurodegenerative diseases.

Keywords

Smynrium connatum, Enzyme inhibition, Antioxidant activity, Extraction

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