Aşırı Kilolu Kişilerde Olası Genotoksik Hasarın Comet Deneyi ile Analizi

Year 2022, Volume: 17 Issue: 3, 43 - 48, 02.11.2022

Gülşen Göney Muhammed Oğuz Halisdemir

https://doi.org/10.17517/ksutfd.942657

Abstract

Özet

Amaç: Son yıllarda yapılmış olan araştırmalar ile obezitenin, DNA zincir kırıklarının onarım mekanizmasını değiştirdiği ortaya çıkarılmıştır. Ayrıca beden kitle indeksinde artış ile genomik kararsızlık arasında ilişki tespit edilmiştir. Sunulan çalışmada aşırı kilolu bireylerin muhtemel genotoksik hasarının araştırılması amaçlanmış olup periferal kan örneklerinde Tek Hücre Jel Elektroforezi deneyi kullanılarak olası genotoksik hasar düzeyi hesaplanmıştır.

Gereç ve Yöntemler: Sunulan çalışmada 18 yaşından büyük aşırı kilolu ya da normal kiloya sahip bireylerin periferal lenfositlerinde olası DNA hasarı Comet Deneyi ile analiz edilmiştir. Sonuçlar SPSS analiz programı kullanılarak istatistiksel olarak karşılaştırılmıştır.

Bulgular: Sunulan araştırmaya yaş ortalaması 30.13±7.97 olan 23 kadın ve yaş ortalaması 38.13±10.63 olan 32 erkek (21 normal kilolu ve 34 aşırı kilolu) toplamda 55 gönüllü katılmıştır. DNA hasarının göstergesi olan kuyruk momenti değeri tüm bireylerde ortalama 1.21±0.45’dir. Aşırı kilolu kişilerin kuyruk momenti değeri ortalama 1.29±0.46 olarak bulunmuştur. Bu değer normal kiloya sahip bireylerin kuyruk momenti sonuçları (1.09±0.40) ile karşılaştırıldığında istatistiksel olarak anlamlı derecede fark bulunmamıştır (p>0.05). Çalışma sonuçlarımıza göre beden kitle indeksinde artış ile DNA hasarı arasında anlamlı

fark bulunmamıştır (p>0.05).

Sonuç: Sunulan çalışma Türkiye’deki yetişkin bireylerde aşırı kiloluluk ve DNA hasar düzeyinin değerlendirildiği ilk çalışma olma özelliğindedir. Sunulan çalışmada Comet Deneyi bulgularına göre beden kitle indeksi ve genotoksik hasar arasında ilişki bulunmadığı tespit edilmiş olup gelecekte DNA hasar düzeyinin farklı genotoksisite testleriyle araştırılacağı yeni çalışmaların yapılması önerilmektedir.

Keywords

Comet deneyi, DNA hasarı, Genotoksisite, Obezite, Tek Tek Hücre Jel Elektroforezi

Investigation of Genotoxic Damage in Overweight Individuals Using the Comet Assay

Abstract

Abstract

Objective: Research has revealed that obesity changed the repair mechanism of DNA chain breaks. Also, the increase in body mass index is found out to be associated with genomic instability. In this study, it was aimed to investigate the possible genotoxic damage of overweight individuals.

Material and Methods: In the present study the level of genotoxic damage was calculated in Turkish adult peripheral blood samples using Single Cell Gel Electrophoresis assay. The results of possible DNA damage levels belonging to overweight people were compared statistically by SPSS analysis program with the results of normal-weight people.

Results: Fifty five volunteers (21 normal weight and 34 overweight); 23 women, mean age=30.13±7.97 and 32 men, mean age=38.13±10.63 participated in the study. Tail moment is an average of 1.21±0.45 in all individuals. Tail moment value was found of overweight people as 1.29±0.46. When this value was compared with the results of individuals with normal weight (1.09±0.40), statistically no significant difference was determined (p>0.05). According to results, no significant difference was found between the increase in body mass index and DNA damage (p>0.05).

Conclusion: The present study is the first study which gives information about the level of DNA damage relation with being overweight in adults of Turkey. In the presented study, it was determined that there was no relationship between body mass index and genotoxic damage according to the findings of the comet assay, and we recommended that new studies should be conducted in the future to investigate the level of DNA damage with different genotoxicity tests.

Keywords

Comet assay, DNA damage, Genotoxicity, Obesity, Single Cell Gel Electrophoresis

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