Age- and Sex- Dependent Changes in Serum Levels of TAS, TOS, TLR2, TLR4, HSP60, HSP90, and HMGB1

Year 2023, Volume: 15 Issue: 1, 105 - 115, 15.03.2023

Salih Tunç Kaya

https://doi.org/10.18521/ktd.1214575

Abstract

Objective: Cellular and physiological functions may be affected in an age- and sex-specific manner. The aim of this study is to investigate sex- and age-specific differences in the serum levels of Total Antioxidant Status (TAS), Total Oxidant Status (TOS), Oxidative Stress Index (OSI), Toll-like Receptor 2 (TLR2), Toll-Like Receptor 4 (TLR4), Heat Shock Protein 60 (HSP60), Heat Shock Protein 90 (HSP90), and High Mobility Group Box 1 (HMGB1) as well as to examine the correlation between them.
Methods: Four groups of mice, each including seven animals, were used in the present study: young males and females (6 months old); old males and females (24 months old). Blood samples were taken from the heart and serum was used to assay the levels of TLR2, TLR4, HSP60, HSP90, HMGB1, TAS and TOS.
Results: HGMB1, TOS and OSI were higher in old females than in young females (p<0.05). TLR2 and TLR4 levels were higher in young females than in young males; however, HSP60 was lower in young females than in young males (p<0.01). HSP60 was lower in old males than in young males (p<0.05). Positive correlations were present between TLR2, TLR4 and HMGB1 (p=0.001, r=0.096; p=0.012, r=0.867; p=0.002, r=0.935, respectively) as well as between HMGB1 and HSP60 (p=0.049, r=0.756) in young females. A negative correlation was detected between HSP90 and TLR4 in young males (p=0.000, r=-0,982), and between HSP60 and TLR2, OSI in old males (p=0.014, r=-0.856; p=0.042, r=-0.772, respectively).
Conclusions: The results of present study indicated that age and sex could be important factors for the serum levels of TLR2, TLR4, HSP60, HSP90, HMGB1 and OSI as well as the correlation between them.

Keywords

Sex, Age, High Mobility Group Box 1, Heat Shock Proteins, Toll Like Receptors, Oxidative Stress

Supporting Institution

Duzce University

Project Number

BAP- 2021.05.01.1262.

Thanks

I would like to thank to Dr. Kağan Ağan for his technical contribution.

TAS, TOS, TLR2, TLR4, HSP60, HSP90 ve HMGB1 Serum Düzeylerinde Yaşa ve Cinsiyete Bağlı Değişiklikler

Abstract

Amaç: Hücresel ve fizyolojik fonksiyonlar yaşa ve cinsiyete özgü bir şekilde etkilenebilir. Bu çalışmanın amacı, Toplam Antioksidan Durumu (TAS), Toplam Oksidan Durumu (TOS), Oksidatif Stres İndeksi (OSI), Toll-Benzeri Reseptör 2 (TLR2), Toll-Benzeri Reseptör 4 (TLR4), Isı Şok Protein 60 (HSP60), Isı Şok Protein 90 (HSP90) ve Yüksek Mobilite Grup Kutusu 1 (HMGB1) serum seviyelerindeki cinsiyete ve yaşa özgü farklılıkları ve aralarındaki korelasyonu incelemektir.
Gereç ve Yöntem: Bu çalışmada her biri yedi hayvan içeren dört fare grubu kullanıldı: genç erkekler ve dişiler (6 aylık); yaşlı erkekler ve dişiler (24 aylık). Kan örnekleri Kalpten alındı ve TLR2, TLR4, HSP60, HSP90, HMGB1, TAS ve TOS seviyelerini değerlendirmek için serum kullanıldı.
Bulgular: HGMB1, TOS ve OSI yaşlı dişilerde genç dişilere göre daha yüksekti (p<0.05). TLR2 ve TLR4 seviyeleri genç dişilerde genç erkeklerden daha yüksekti; ancak HSP60 genç dişilerde genç erkeklere göre daha düşüktü (p<0.01). HSP60 yaşlı erkeklerde genç erkeklere göre daha düşüktü (p<0.05). Genç dişilerde TLR2, TLR4 ve HMGB1 arasında (sırasıyla p=0,001, r=0,096; p=0,012, r=0,867; p=0,002, r=0,935) ve HMGB1 ile HSP60 arasında (p=0,049, r=0,756) pozitif korelasyonlar mevcuttu. Genç erkeklerde HSP90 ile TLR4 arasında (p=0.000, r=-0,982), yaşlı erkeklerde HSP60 ile TLR2 ve OSI arasında negatif korelasyon saptandı (sırasıyla (p=0,014, r=-0,856; p=0,042, r=-0,772)
Sonuç: Bu çalışmanın sonuçları, TLR2, TLR4, HSP60, HSP90, HMGB1 ve OSI'nin serum düzeyleri ve aralarındaki korelasyon için yaş ve cinsiyetin önemli faktörler olabileceğini göstermiştir.

Keywords

Cinsiyet, Yaş, Yüksek Mobilite Grup Kutusu 1, Isı Şok Proteinleri, Toll Benzeri Reseptörler, Oksidatif Stres

Project Number

BAP- 2021.05.01.1262.

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