kırıkkale uni med j The Journal of Kırıkkale University Faculty of Medicine 2148-9645 Kirikkale University 10.24938/kutfd.1184139 Health Care Administration Sağlık Kurumları Yönetimi THE INVESTIGATION OF THE EFFECTS OF VITAMIN D AND OMEGA 3 ON VENA CAVA Vitamin D ve Omega 3’ün Vena Kava Üzerine Etkilerinin Araştırılması https://orcid.org/0000-0001-9558-4122 Kartal Bahar Ankara Yıldırım Beyazıt University, Faculty of Medicine, Dept of Histology and Embryology https://orcid.org/0000-0002-9557-3631 Alimoğulları Ebru Ankara Yıldırım Beyazıt University, Faculty of Medicine, Dept of Histology and Embryology https://orcid.org/0000-0002-1669-0988 Bozkurt Mehmet Fatih Afyon Kocatepe University Faculty of Veterinary Medicine, Dept of Pathology https://orcid.org/0000-0001-7696-3039 Bhaya Muhammad Nasir AFYON KOCATEPE UNIVERSITY https://orcid.org/0000-0002-2278-2651 Demir Hazal Ankara Yıldırım Beyazıt University, Faculty of Medicine, Dept of Histology and Embryology https://orcid.org/0000-0002-3154-8779 Saçık Uygar Ankara Yıldırım Beyazıt University, Faculty of Medicine, Dept of Histology and Embryology 04 30 2023 25 1 100 106 10 04 2022 03 10 2023 Copyright © 1999, The Journal of Kırıkkale University Faculty of Medicine 1999 The Journal of Kırıkkale University Faculty of Medicine

Objective: Vitamin D has a protective role in the cardiovascular system and it affects blood pressure. Omega-3 fatty acids are dietary fats gained from fish and plant oils and involve in coronary heart disease and other cardiovascular complications. The aim of the study was to investigate the effects of Vitamin D and Omega 3 on the vascular structure at the cellular level.Material and Methods: In the current study, a total of 24 rats were divided into 4 groups. Each group contained 6 animals. The groups are as follows; control, vitamin D, Omega 3; and combined Vitamin D and Omega 3. Vena cava samples from all groups were obtained and stained with hematoxylin and eosin (H&E) for histological alternations. Additionally, endothelial and vascular functions were investigated immunohistochemically.Results: The H&E staining revealed that the treatment of either Vitamin D or Omega 3 did not cause histomorphological changes in the structure of the vena cava under normal conditions. The immunoexpression of inducible nitric oxide synthase was decreased and vascular endothelial growth factor was increased in the vena cava of rats with the combined treatment of Vitamin D and Omega 3.Conclusion: In conclusion, combined supplements of Vitamin D and Omega 3 did not have harmful effects on the blood vessel however further studies should be performed to determine the beneficial effects of these supplements.

Amaç: D vitamini kardiyovasküler sistem üzerinde koruyucu bir role sahiptir ve kan basıncını etkiler. Omega-3 yağ asitleri balık ve bitki yağlarından elde edilir ve koroner kalp hastalığına ve diğer kardiyovasküler komplikasyonlarda önemli rolü vardır. Çalışmamızın amacı, D vitamini ve Omega 3'ün damar yapısı üzerindeki etkilerinin hücresel düzeyde araştırmaktır.Gereç ve Yöntemler: Mevcut çalışmada toplam 24 sıçan kullanıldı. Her biri 6 hayvandan oluşan kontrol, D vitamini, Omega 3; ve kombine D Vitamini ve Omega 3 tedavisi uygulanan olmak üzere 4 grup belirlendi. Deney sonunda tüm gruplardan vena cava örnekleri alındı. Dokular histolojik değişimler için analiz edildi. Endotelyal ve vasküler fonksiyonlar immünhistokimyasal yöntem ile incelendi.Bulgular: D Vitamini veya Omega 3 kullanımının normal koşullar altında vena cava yapısında histomorfolojik değişikliklere yol açmadığı tespit edildi. Vitamin D ve Omega 3'ün birlikte kullanımı sonucunda sıçanların vena cava örneklerinde indüklenebilir nitrik oksit sentazın immünoekspresyonu azaltırken, vasküler endotelyal büyüme faktörünün arttığı tespit edildi.Sonuç: Sonuç olarak, D vitamini ve Omega 3'ün kombine takviyelerinin hücresel düzeyde damar yapısı üzerine zararlı bir etkisi olmadığına ancak faydalarının tespit edilmesi için daha kapsamlı çalışmalar yapılması kanaatine varıldı.

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