ÇUHA ÇİÇEĞİ VE SARI KANTARON YAĞLARININ MULTİPLE SKLEROZ MODELİ OLUŞTURULMUŞ FARELERDEKİ JAK/STAT SİNYAL YOLAKLARI ÜZERİNE ETKİSİ

Year 2022, Volume: 24 Issue: 2, 225 - 235, 31.08.2022

Huri Bulut , Emine Şeyda Teloğlu , Hilal Şentürk , Savaş Üstünova , Zozan Güleken , Şahabettin Selek

https://doi.org/10.24938/kutfd.986098

Abstract

Amaç: Multipl Skleroz, merkezi sinir sisteminde inflamatuar infiltrasyonlarla ilerleyen otoimmün demiyelinizan bir hastalıktır. JAK-STAT sinyal yolunun düzensizliği, otoimmün ensefalomiyelit ile indüklenmiş Multipl Skleroz modellerinin patogenezinde önemli bir rol oynar. Bu çalışmanın amacı, sarı kantaron ve çuha çiçeği yağlarının tüketiminin, JAK-STAT sinyal yolu üzerindeki terapötik etkilerini ortaya çıkarmaktır.

Gereç ve Yöntemler: Çalışmamızda 42 adet dokuz haftalık C57bl/6 J fareler kullanıldı. Fareler, Multipl Skleroz (MS) (n=32) ve kontrol grubu (n=10) olmak üzere iki ana gruba ayrıldıktan sonra MS grubundaki tüm farelere deneysel otoimmün ensefalomiyelit yöntemi ile Multipl Skleroz hastalığı oluşturuldu. Multipl Skleroz grubu kendi içinde MS (n=7), MS + Çuha çiçeği (n=10) ve MS + Sarı Kantaron (n=15) olacak şekilde üç gruba ayrıldı. Tedavi grupları, 6 hafta boyunca sarı kantaron (20 g/kg) ve çuha çiçeği (20 g/kg) yağları içeren özel üretim yemler ile beslendi. Multipl Skleroz indüksiyonundan iki hafta sonra, hastalığın klinik belirtileri her fare için günlük olarak puanlandı. Çalışmamızın sonunda beyin dokusu örneklerini elde etmek için tüm gruplardaki fareler sakrifiye edildi. Beyin doku homojenatlarında Western Blot yöntemi ile JAK2, p-JAK2, STAT1 ve p-STAT1 protein ekspresyon seviyeleri ölçüldü.

Bulgular: Çalışmamızda Multipl Skleroz hastalığı patogenezi ile ilişkili JAK/STAT yolağındaki p-JAK2, JAK2, p-STAT1 ve STAT1 ekspresyon düzeylerinin MS grubunda kontrollere göre anlamlı olarak arttığını, sarı kantaron ve çuha çiçeği yağı ile beslenen gruplarda ise anlamlı olarak azaldığını gösterdik (p<0.05).

Sonuç: Bu çalışmada elde ettiğimiz veriler ışığında, özellikle çuha çiçeği ve sarı kantaron yağlarının besin takviyesi olarak tüketilmesinin, Multipl Skleroz hastalığının moleküler patogenezinin iyileşmesine katkı sağlayacağı söylenebilir.

Keywords

Multipl Skleroz, çuha çiçeği (oenothera biennis),, sarı kantaron (hypericum perforatum),, JAK/STAT sinyal yolağı

Supporting Institution

Bezmialem Vakıf Üniversitesi

The Effect of Evening Primrose and St. John's Wort Oils on Jak/STAT Signaling Pathways in Mice with Multiple Sclerosis Modeled

Abstract

Objective: Multiple Sclerosis is an autoimmune demyelinating disease that progresses with inflammatory infiltrates in the central nervous system. Dysregulation of the JAK-STAT signaling pathway plays an important role in the pathogenesis of experimental autoimmune encephalomyelitis-induced Multiple Sclerosis models. The aim of this study was to reveal the therapeutic effects of the consumption of St. John's Wort and evening primrose oils on the JAK-STAT signaling pathway.

Material and Methods: In our study, 42 nine-week-old C57bl/6 J mice were used and divided into two groups as Multiple Sclerosis (MS) (n = 32) and control group (n = 10). MS group was divided into three groups as MS (n=7), MS + evening primrose oil (n=10) and MS + St. John's Wort oil (n= 15). All groups except the control group were immunized by experimental autoimmune encephalomyelitis methods. Treatment groups were fed special feeds containing 20 g/kg St. John's Wort and 20 g/kg evening primrose oils for 6 weeks. Two weeks after Multiple Sclerosis induction, clinical signs of disease were scored daily for each mouse. Brain tissue samples were collected for measurement of JAK2, p-JAK2, STAT1 and p-STAT1 protein expression level by Western blot method.

Results: We showed that the expression levels of p-JAK2, JAK2, p-STAT1 and STAT1 in the JAK/STAT pathway associated with the pathogenesis of Multiple Sclerosis disease increased significantly in the Multiple Sclerosis group compared to the controls and decreased significantly in the groups fed with St. John's wort and evening primrose oil (p<0.05).

Conclusion: In the light of the data, consumption of evening primrose and alternatively St. John's wort oils as nutritional supplements may contribute to the improvement of the molecular pathogenesis of Multiple Sclerosis disease.

Keywords

Multiple Sclerosis,, Evening Primrose (Oenothera biennis),, St. John's Wort (Hypericum perforatum),, JAK/STAT signalling pathway

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