kvj Kocatepe Veterinary Journal 2147-6853 Afyon Kocatepe University 10.30607/kvj.1157121 Veterinary Sciences Veterinary Surgery Veteriner Bilimleri Veteriner Cerrahi Cynara scolymus L. Yaprağının Farklı Ekstraktlarda In vitro Aktioksidan Aktivitelerinin ve Makro ve Mikro Element Seviyelerinin Belirlenmesi Determination of In vitro Antioxidant Activities and Macro and Micro Elements Level in Different Extracts of Cynara Scolymus L. leaf https://orcid.org/0000-0001-6523-2257 Albayrak Sümeyra TÜRKİYE iLAÇ VE TIBBİ CİHAZ KURUMU https://orcid.org/0000-0002-7586-0895 Denk Barış AFYON KOCATEPE ÜNİVERSİTESİ https://orcid.org/0000-0001-6095-5210 Karpuz Büşra BAŞKENT ÜNİVERSİTESİ ECZACILIK FAKÜLTESİ FARMAKOGNOZİ ABD https://orcid.org/0000-0002-5829-7869 Küpeli Akkol Esra GAZI UNIVERSITY https://orcid.org/0000-0001-8767-4507 Avcı Gülcan AFYON KOCATEPE ÜNİVERSİTESİ 12 31 2022 15 4 412 422 08 09 2022 11 15 2022 Copyright © 2008, Kocatepe Veterinary Journal 2008 Kocatepe Veterinary Journal

Papatyagiller familyasına ait olan Küre enginarın (Cynara scolymus L.) antioksidan, hepatoprotektif ve hipoglisemik etkilere sahiptir, yaprakları geleneksel olarak idrar söktürücü ve koleretik amaçlarla kullanılmaktadır. Bu nedenle bu çalışmanın temel amacı enginar yaprağının ve yaprağın metanollü, etil asetatlı ve n-hekzanlı ekstraktlarının toplam fenolik içeriğini bazı in vitro antioksidan aktiviteleri, seçilmiş makro (Na, K, Ca, Mg, P) ve mikro (Zn, Cu, Mn, Cr, Se, I) element düzeylerini, belirlemektir. Toplam fenolik içerik değerlerine göre metanol, etil asetatlı ve n-hekzan ekstraktları sırasıyla 5,375 mg, 0,917 mg, 0,167 mg Gallik asit (GAE)/g bulunmuştur. Metanol ekstraktı en yüksek DPPH serbest radikal süpürme aktivitesi (%87,73) gösterirken, etil asetat ekstraktı en yüksek süperoksit radikal süpürme aktivitesine (%49,02) sahip iken n-hekzan ekstraktı yüksek seviye metal şelatlama kapasitesi (289,32 µM Fe) içermektedir. Makro ve mikro elementler açısından (I ve Cr seviyeleri hariç) en yüksek konsantrasyonlar doğal mineral kaynağı olarak kabul edilen yapraklarında kaydedilmiştir. Buna göre enginar yapraklarının potansiyel bir doğal K ve Zn kaynağı sağladığı, metanol ve n-hekzan ekstraktlarının ise iyi P ve Zn kaynağı olduğu değerlendirilmektedir.

Globe Artichoke (Cynara scolymus L.) belonging to the family of Astericaea, has antioxidant, hepatoprotective and hypoglycemic activities, its leaves have traditionally been used for diuretic and choleretic purposes. Therefore the main goal of this study is to determine the total phenolic content of artichoke leaf and its extracts with methanol, ethyl acetate and n- hexane, some in vitro antioxidant activities, selected macro (Na, K, Ca, Mg, P) and micro (Zn, Cu, Mn, Cr, Se, I) elements’ levels. Accordingly the total phenolic content values for the methanol, ethyl acetate and N-hexane extracts found 5,375 mg, 0,917 mg, 0,167 mg Gallic acid (GAE)/g respectively. Methanol extract showed highest DPPH free radical scavenging activity (87,73%), ethyl acetate extract possessed the highest Superoxide radical scavenging activity (SRSA) (49,02 %) whereas N-hexane extract contained high level metal chelating ability ( 289,32µM Fe). In terms of macro and micro elements (except I and Cr levels), the highest concentrations are recorded in its leaves, which are considered as a natural mineral source. Accordingly, it is evaluated that artichoke leaves provide a potential natural sources of K and Zn, while methanol and N-hexane extracts are good sources of P and Zn.

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