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            <front>

                <journal-meta>
                                                                <journal-id>kvj</journal-id>
            <journal-title-group>
                                                                                    <journal-title>Kocatepe Veterinary Journal</journal-title>
            </journal-title-group>
                                        <issn pub-type="epub">2147-6853</issn>
                                                                                            <publisher>
                    <publisher-name>Afyon Kocatepe University</publisher-name>
                </publisher>
                    </journal-meta>
                <article-meta>
                                        <article-id pub-id-type="doi">10.30607/kvj.1282056</article-id>
                                                                <article-categories>
                                            <subj-group  xml:lang="en">
                                                            <subject>Veterinary Sciences</subject>
                                                    </subj-group>
                                            <subj-group  xml:lang="tr">
                                                            <subject>Veteriner Bilimleri</subject>
                                                    </subj-group>
                                    </article-categories>
                                                                                                                                                        <title-group>
                                                                                                                        <trans-title-group xml:lang="tr">
                                    <trans-title>Simmental Irkı İneklerde Optimum Gönüllü Bekleme Süresi ve Senkronizasyon Programının Belirlenmesi</trans-title>
                                </trans-title-group>
                                                                                                                                                                                                <article-title>Determining the optimum voluntary waiting period and synchronization protocol in Simmental cows</article-title>
                                                                                                    </title-group>
            
                                                    <contrib-group content-type="authors">
                                                                        <contrib contrib-type="author">
                                                                    <contrib-id contrib-id-type="orcid">
                                        https://orcid.org/0000-0002-7977-6826</contrib-id>
                                                                <name>
                                    <surname>Kara</surname>
                                    <given-names>Uğur</given-names>
                                </name>
                                                                    <aff>Doğu Akdeniz Tarımsal Araştırma Enstitüsü</aff>
                                                            </contrib>
                                                    <contrib contrib-type="author">
                                                                    <contrib-id contrib-id-type="orcid">
                                        https://orcid.org/0000-0002-8959-5658</contrib-id>
                                                                <name>
                                    <surname>Düzer</surname>
                                    <given-names>Mehmet</given-names>
                                </name>
                                                            </contrib>
                                                    <contrib contrib-type="author">
                                                                    <contrib-id contrib-id-type="orcid">
                                        https://orcid.org/0000-0002-5451-1397</contrib-id>
                                                                <name>
                                    <surname>Hızlı</surname>
                                    <given-names>Hatice</given-names>
                                </name>
                                                                    <aff>DOĞU AKDENİZ TARIMSAL ARAŞTIRMA ENSTİTÜSÜ</aff>
                                                            </contrib>
                                                    <contrib contrib-type="author">
                                                                    <contrib-id contrib-id-type="orcid">
                                        https://orcid.org/0000-0002-6884-2621</contrib-id>
                                                                <name>
                                    <surname>Baki Acar</surname>
                                    <given-names>Duygu</given-names>
                                </name>
                                                                    <aff>AFYON KOCATEPE ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ</aff>
                                                            </contrib>
                                                                                </contrib-group>
                        
                                        <pub-date pub-type="pub" iso-8601-date="20230930">
                    <day>09</day>
                    <month>30</month>
                    <year>2023</year>
                </pub-date>
                                        <volume>16</volume>
                                        <issue>3</issue>
                                        <fpage>401</fpage>
                                        <lpage>409</lpage>
                        
                        <history>
                                    <date date-type="received" iso-8601-date="20230412">
                        <day>04</day>
                        <month>12</month>
                        <year>2023</year>
                    </date>
                                                    <date date-type="accepted" iso-8601-date="20230821">
                        <day>08</day>
                        <month>21</month>
                        <year>2023</year>
                    </date>
                            </history>
                                        <permissions>
                    <copyright-statement>Copyright © 2008, Kocatepe Veterinary Journal</copyright-statement>
                    <copyright-year>2008</copyright-year>
                    <copyright-holder>Kocatepe Veterinary Journal</copyright-holder>
                </permissions>
            
                                                                                                <trans-abstract xml:lang="tr">
                            <p>Bu çalışmanın amacı, Simental ırkı ineklerde farklı gönüllü bekleme sürelerinin (GBS) sonunda dört farklı senkronizasyon programının etkinliğinin karşılaştırılması, doğum sayısı ve süt veriminin bu senkronizasyon protokolleri ile gebelik oranlarına etkilerinin değerlendirilmesidir. Hayvanlar rastlantısal olarak dört farklı senkronizasyon ve bir kontrol grubuna ayrıldı. Doğum sayısı, süt verimi (≤23 kg/gün; &amp;gt;23 kg/gün) ve sağılan gün sayısının (SGS; 45-60, 61-90, &amp;gt;90 günler) uygulanan senkronizasyon protokollerine etkileri karşılaştırıldı. Ovsynch (Grup I; n=81), Ovsynch+Progesteron (Grup II; n=30), G6G (Grup III; n=66), 2xG6G (Grup IV; n=45) ve kontrol (n=35) grupları oluşturuldu. Doğum sayısının gebelik oranlarına etkisi karşılaştırıldığında, ilk laktasyondaki primipar ineklerde multipar ineklere göre özellikle Grup II’de istatistiksel önemin (P0,05), süt veriminin de gebelik oranı üzerinde istatistiksel öneme yol açmadığı (P&amp;gt;0,05) belirlendi. Sonuç olarak, Simental ırkı ineklerin Ovsynch, Ovsynch+Progesteron, G6G ve 2xG6G protokolleri ile senkronizasyonunun farklı sağılan gün sayısı (SGS)’na göre gebelik oranlarını etkilemediği belirlendi. Sunulan çalışmadan elde edilen veriler veteriner hekimlerin pratik, etkili, uygun maliyetli ve çiftliğin özel ihtiyaçlarını karşılayabilecek yukarıda belirtilen herhangi bir senkronizasyon protokolünü seçebileceğini, en uygun gönüllü bekleme süresinin ise çiftliğin ekonomik ve bölgesel piyasa hedeflerine göre planlanmasının daha etkili ve verimli olacağını ortaya koymaktadır.</p></trans-abstract>
                                                                                                                                    <abstract><p>The objective of the present study was to compare four different synchronization protocols at the end of various voluntary waiting period (VWP) and evaluate the effect of parity and milk yield on pregnancy rates with synchronization in Simmental cows. All animals were randomly allocated into four synchronization groups and one control group. Parity, milk yield (≤23 kg/d; &amp;gt;23 kg/d), and days in milk (DIM; 45-60, 61-90, &amp;gt;90 days) were compared according to synchronization protocols. Ovsynch (Group I; n=81), Ovsynch+Progesterone (Group II; n=30), G6G (Group III; n=66), 2xG6G (Group IV; n=45), and a control group (n=35) were designed for the study. The parity significantly affected the pregnancy rates in the primiparous cows, especially in Group II (P0.05). There was no significant difference between milk yield and pregnancy rates (P&amp;gt;0.05). In conclusion, the synchronization of Simmental cows with Ovsynch, Ovsynch+Progesterone, G6G, or 2xG6G did not affect pregnancy rates across various DIM. These data suggest that the suitable VWP could be planned according to the farm’s economic and local market targets, with veterinarians able to choose a protocol based on practicality, effectiveness and cost, as well as the specific requirements of the herd.</p></abstract>
                                                            
            
                                                                                        <kwd-group>
                                                    <kwd>Simmental Cow</kwd>
                                                    <kwd>  Synchronization</kwd>
                                                    <kwd>  Woluntary Waiting Period</kwd>
                                            </kwd-group>
                            
                                                <kwd-group xml:lang="tr">
                                                    <kwd>Simental İnek</kwd>
                                                    <kwd>  Senkronizasyon</kwd>
                                                    <kwd>  Gönüllü Bekleme Süresi</kwd>
                                            </kwd-group>
                                                                                                                                    <funding-group specific-use="FundRef">
                    <award-group>
                                                    <funding-source>
                                <named-content content-type="funder_name">No support was received during this study.</named-content>
                            </funding-source>
                                                                    </award-group>
                </funding-group>
                                </article-meta>
    </front>
    <back>
                            <ref-list>
                                    <ref id="ref1">
                        <label>1</label>
                        <mixed-citation publication-type="journal">Arbel, R., Bigun, Y., Ezra, E., Sturman, H., Hojman, D. (2001). The effect of extended calving intervals in high lactating cows on milk production and profitability. Journal of Dairy Science, 84, 600–608.</mixed-citation>
                    </ref>
                                    <ref id="ref2">
                        <label>2</label>
                        <mixed-citation publication-type="journal">Astiz, S., Fargas, O. (2013). Pregnancy per AI differences between primiparous and multiparous high-yield dairy cows after using Double Ovsynch or G6G. Theriogenology, 79, 1065-1070.</mixed-citation>
                    </ref>
                                    <ref id="ref3">
                        <label>3</label>
                        <mixed-citation publication-type="journal">Ben Salem, M., Djemali, M., Kayouli, C., Majdoup. (2006). A review of environmental and management factors affecting the reproductive performance of Holstein-Friesian dairy herds in Tunisia. Livestock Research for Rural Development, 18, 53.</mixed-citation>
                    </ref>
                                    <ref id="ref4">
                        <label>4</label>
                        <mixed-citation publication-type="journal">Bo, G.A., de la Mata, J.J., Baruselli, P.S., Menchaca, A. (2016). Alternative programs for synchronizing and resynchronizing ovulation in beef cattle. Theriogenology, 86, 388-396.</mixed-citation>
                    </ref>
                                    <ref id="ref5">
                        <label>5</label>
                        <mixed-citation publication-type="journal">Butler, S.T., Cummins, S.B., Herlihy, M.M., Hutchinson, I.A., Moore, S.G. (2019). Optimizing productive and reproductive performance in the grazing cow. Bioscientifica Proceedings, 8, 179-191.</mixed-citation>
                    </ref>
                                    <ref id="ref6">
                        <label>6</label>
                        <mixed-citation publication-type="journal">Fleming, A., Ebdalla, E.A., Maltecca, C., Baes, C.F. (2018). Invited review: Reproductive and genomic technologies to optimize breeding strategies for genetic progress in dairy cattle. Archives Animal Breeding, 61, 43–57.</mixed-citation>
                    </ref>
                                    <ref id="ref7">
                        <label>7</label>
                        <mixed-citation publication-type="journal">Fricke, P.M., Giordano, J.O., Valenza, A., Amundson, V.C., Carvalho, P.D. (2014). Reproductive performance of lactating dairy cows managed for first service using timed artificial insemination with or without detection of estrus using an activity-monitoring system. Journal of Dairy Science,  97, 2771-2781.</mixed-citation>
                    </ref>
                                    <ref id="ref8">
                        <label>8</label>
                        <mixed-citation publication-type="journal">Giordano, J.O., Fricke, P.M., Guenther, J.N., Lopes Jr, G., Herlihy, M.M., Nascimento, A.B., Wiltbank, MC. (2012). Effect of progesterone on magnitude of the luteinizing hormone surge induced by two different doses of GnRH in lactating dairy cows. Journal of Dairy Science, 95, 3781–3793.</mixed-citation>
                    </ref>
                                    <ref id="ref9">
                        <label>9</label>
                        <mixed-citation publication-type="journal">Giordano, J.O., Wiltbank, M.C., Fricke, P.M., Bas, S., Pawlisch, R., Guenther, J.N., Nascimento, A.B. (2013). Effect of increasing GnRH and PGF2α dose during Double-Ovsynch on ovulatory response, luteal regression, and fertility of lactating dairy cows. Theriogenology, 80, 773-783.</mixed-citation>
                    </ref>
                                    <ref id="ref10">
                        <label>10</label>
                        <mixed-citation publication-type="journal">Heidari, F., Dirandeh, E., Ansari Pirsaraei, Z., Colazo, M.G. (2017). Modifications of the G6G timed-AI protocol improved pregnancy per AI and reduced pregnancy loss in lactating dairy cows. Animal, 11, 2002-2009.</mixed-citation>
                    </ref>
                                    <ref id="ref11">
                        <label>11</label>
                        <mixed-citation publication-type="journal">Herlihy, M.M., Giordano, J.O., Souza, A.H., Ayres, H., Ferreira, R.M., Keskin, A., Nascimento, A.B., Guenther, J.N., Gaska, J.M., Kacuba, S.J., Crowe, M.A., Butler, S.T., Wiltbank, M.C. (2012). Presynchronization with Double-Ovsynch improves fertility at first postpartum artificial insemination in lactating dairy cows. Journal of Dairy Science, 95, 7003-7014.</mixed-citation>
                    </ref>
                                    <ref id="ref12">
                        <label>12</label>
                        <mixed-citation publication-type="journal">Herlihy, M.M., Crowe, M.A., Berry, D.P., Diskin, M.G., Butler, S.T. (2013). Factors associated with fertility outcomes in cows treated with protocols to synchronize estrus and ovulation in seasonal-calving, pasture-based dairy production systems. Journal of Dairy Science, 96, 1485-1498.</mixed-citation>
                    </ref>
                                    <ref id="ref13">
                        <label>13</label>
                        <mixed-citation publication-type="journal">Inchaisri, C., Jorritsma, R., Vos, P., van der Weijden, G.C., Hogeveen, G. (2011). Analysis of the economically optimal voluntary waiting period for first insemination. Journal of Dairy Science, 94, 3811–3823.</mixed-citation>
                    </ref>
                                    <ref id="ref14">
                        <label>14</label>
                        <mixed-citation publication-type="journal">Kaçar, C., Kamiloglu, N.N., Ucar, O., Arı, U.C. (2008). İneklerde β-karoten+ E vitamini uygulamasıyla kombine edilen Ovsynch ve Cosynch senkronizasyon programlarının gebelik oranı üzerine etkisi. Kafkas Universitesi Veteriner Fakültesi Dergisi, 14(1), 45-50.</mixed-citation>
                    </ref>
                                    <ref id="ref15">
                        <label>15</label>
                        <mixed-citation publication-type="journal">Kohsari, H., Berenjian, K. (2022). Comparison of the effects of Presynch-Ovsynch, Presynch-Ovsynch+ CIDR, and G6G protocols on the reproductive performance of lactating Holstein cows in the postpartum period. Veterinarska Stanica, 53, 419-430.</mixed-citation>
                    </ref>
                                    <ref id="ref16">
                        <label>16</label>
                        <mixed-citation publication-type="journal">Larson, J.A., Lamb, G.C., Stevenson, J.S., Johnson, S.K., Day, M.L., Geary, T.W., Kesler, D.J., DeJarnette, J.M., Schrick, F.N., DiCostanzo, A., Arseneau, J.D. (2006). Synchronization of estrus in suckled beef cows for detected estrus and artificial insemination using gonadotropin-releasing hormone, prostaglandin F, and progesterone. Journal of Animal Science, 84, 332-342.</mixed-citation>
                    </ref>
                                    <ref id="ref17">
                        <label>17</label>
                        <mixed-citation publication-type="journal">Lindley, G., Willshire, J., Martin, M. (2021a). Controlled breeding in dairy cows. Part 1: bovine oestrus cycle and synchronization. In Practice, 43, 445-456.</mixed-citation>
                    </ref>
                                    <ref id="ref18">
                        <label>18</label>
                        <mixed-citation publication-type="journal">Lindley, G., Willshire, J., Martin, M. (2021b). Controlled breeding in dairy cows. Part 2: selection and ethical implications. In Practice, 43, 511-518.</mixed-citation>
                    </ref>
                                    <ref id="ref19">
                        <label>19</label>
                        <mixed-citation publication-type="journal">Martinez-Castillero, M., Toledo-Alvarado, H., Pegolo, S., Vazquez, A.I., de los Campos, G., Varona, L., Finocchiaro, R., Bittante, G., Cecchinato, A. (2020). Genetic parameters for fertility traits assessed in herds divergent in milk energy output in Holstein-Friesian, Brown Swiss, and Simmental cattle. Journal of Dairy Science, 103, 11545-11558.</mixed-citation>
                    </ref>
                                    <ref id="ref20">
                        <label>20</label>
                        <mixed-citation publication-type="journal">Meikle, A., Kulcsar, M., Chilliard, Y., Febel, H., Delavaud, C., Cavestany, D., Chilibroste, P. (2004). Effects of parity and body condition at parturition on endocrine and reproductive parameters of the cow. Reproduction, 127, 727-737.</mixed-citation>
                    </ref>
                                    <ref id="ref21">
                        <label>21</label>
                        <mixed-citation publication-type="journal">Nak, Y., Tuna, B., Nak, D.,  Karakas, E., Simsek, G. (2011). The effects of Ovsynch, Ovsynch with progestin and progestin plus double TAI on pregnancy rates in unobserved oestrus dairy cows and heifers. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 17(6), 917-922.</mixed-citation>
                    </ref>
                                    <ref id="ref22">
                        <label>22</label>
                        <mixed-citation publication-type="journal">NRC. (2001). Nutrient Requirements of Dairy Cattle. 7th Rev Ed Natl Acad Sci, Washington, DC.
Pursley, J.R., Mee, M.O., Wiltbank, M.C. (1995). Synchronization of ovulation in dairy cows using PGF2α and GnRH. Theriogenology, 44, 915–923.</mixed-citation>
                    </ref>
                                    <ref id="ref23">
                        <label>23</label>
                        <mixed-citation publication-type="journal">Pursley, J.R., Wiltbank, M.C., Stevenson, J.S., Ottobre, J.S., Garverick, H.A., Anderson, L.L. (1997). Pregnancy rates per artificial insemination for cows and heifers inseminated at a synchronized ovulation or synchronized estrus. Journal of Dairy Science, 80, 295-300.</mixed-citation>
                    </ref>
                                    <ref id="ref24">
                        <label>24</label>
                        <mixed-citation publication-type="journal">Ribeiro, E.S., Bisinotto, R.S., Favoreto, M.G., Martins, L.T., Cerri, L.R.A., Silvestre, F.T., Greco, L.F., Thatcher, W.W., Santos, J.E.P. (2012). Fertility in dairy cows following presynchronization and administering twice the luteolytic dose of prostaglandin F2a as one or two injections in the 5-day timed artificial insemination protocol. Theriogenology, 78, 273–284.</mixed-citation>
                    </ref>
                                    <ref id="ref25">
                        <label>25</label>
                        <mixed-citation publication-type="journal">Ribeiro, E.S., Cerri, R.L.A., Bisinotto, R.S., Lima, F.S., Silvestre, F.T., Greco, L.F., Thatcher, W.W., Santos, J.E.P. (2011). Reproductive performance of grazing dairy cows following presynchronization and resynchronization protocols. Journal of Dairy Science, 94, 4984-4996.</mixed-citation>
                    </ref>
                                    <ref id="ref26">
                        <label>26</label>
                        <mixed-citation publication-type="journal">Sartori, R., Fricke, P.M., Ferreira, J.C., Ginther, O.J., Wiltbank, M.C. (2001). Follicular deviation and acquisition of ovulatory capacity in bovine follicles. Biology of Reproduction, 65, 1403-1409.</mixed-citation>
                    </ref>
                                    <ref id="ref27">
                        <label>27</label>
                        <mixed-citation publication-type="journal">Shahzad, A.H., Sattar, A., Ahmad, N., Ahmad, I., Nak, D., Nak, Y. (2019). Evaluation of Ovsynch and CIDR+Ovsynch protocols to improve reproductive efficiency in lactating dairy cows. Pakistan Journal of Zoology, 51, 1607-1614.</mixed-citation>
                    </ref>
                                    <ref id="ref28">
                        <label>28</label>
                        <mixed-citation publication-type="journal">Souza, A.H., Cunha, A.P., Silva, E.P., Gümen, A., Ayres, H., Guenther, J.N., Wiltbank, M.C. (2009). Comparison of gonadorelin products in lactating dairy cows: efficacy based on induction of ovulation of an accessory follicle and circulating luteinizing hormone profiles. Theriogenology, 72, 271–279.</mixed-citation>
                    </ref>
                                    <ref id="ref29">
                        <label>29</label>
                        <mixed-citation publication-type="journal">Stangaferro, M.L., Wijma, R., Masello, M., Thomas, M., Giordano, J.O. (2018). Economic performance of lactating dairy cows submitted for first service timed artificial insemination after a voluntary waiting period of 60 or 88 days. Journal of Dairy Science, 101, 7500-7516.</mixed-citation>
                    </ref>
                                    <ref id="ref30">
                        <label>30</label>
                        <mixed-citation publication-type="journal">Thatcher, W.W., Bilby, T.R., Bartolome, J.A., Silvestre, F., Staples, C.R., Santos, J.E.P. (2006). Strategies for improving fertility in the modern dairy cow. Theriogenology, 65, 30–44.</mixed-citation>
                    </ref>
                                    <ref id="ref31">
                        <label>31</label>
                        <mixed-citation publication-type="journal">Toledo-Alvarado, H., Cecchinato, A., Bittante, G. (2017). Fertility traits of Holstein, Brown Swiss, Simmental, and Alpine Grey cows are differently affected by herd productivity and milk yield of individual cows. Journal of Dairy Science, 100, 1-12.</mixed-citation>
                    </ref>
                                    <ref id="ref32">
                        <label>32</label>
                        <mixed-citation publication-type="journal">Wiltbank, M.C., Baez, G.M., Cochrane, F., Barletta, R.V., Trayford, C.R., Joseph, R.T. (2015). Effect of a second treatment with prostaglandin F2α during the Ovsynch protocol on luteolysis and pregnancy in dairy cows. Journal of Dairy Science, 98, 8644–8654.</mixed-citation>
                    </ref>
                                    <ref id="ref33">
                        <label>33</label>
                        <mixed-citation publication-type="journal">Yılmaz, C., Yılmaz, O., Ucar, M. (2011). Effect of PGF2α and GnRH injections applied before Ovsynch on pregnancy rates in cows and heifers. Kafkas Universitesi Veteriner Fakültesi Dergisi, 17(4), 641-644.</mixed-citation>
                    </ref>
                            </ref-list>
                    </back>
    </article>
