kvj Kocatepe Veterinary Journal 2147-6853 Afyon Kocatepe University 10.30607/kvj.1319111 Animal Science, Genetics and Biostatistics Zootekni, Genetik ve Biyoistatistik The Effects of High Calcium and Vitamin D on the Fructose- Induced Lipogenesis Pathway in Rats Ratlarda Yüksek Kalsiyum ve Vitamin D’nin Fruktozla İndüklenmiş Lipogenez Yolağı Üzerine Etkileri https://orcid.org/0000-0003-2272-0968 Kara Semiha Özge Hatay Mustafa Kemal Üniversitesi https://orcid.org/0000-0002-9248-828X Yakan Akın Hatay Mustafa Kemal University 12 31 2023 16 4 541 555 06 23 2023 12 08 2023 Copyright © 2008, Kocatepe Veterinary Journal 2008 Kocatepe Veterinary Journal

In this study, the protective effects of high amounts of dietary calcium and vitamin D on fructose-induced lipogenesis were investigated at molecular and biochemical levels. Control group (Con, n=8), High fructose diet group (F, n=8), High fructose diet+75 mg CaCO3 and 26.4 IU vitamin D3 group (FCaD1, n=8), High fructose diet+150 mg CaCO3 and 52.8 IU vitamin D3 group (FCaD2, n=8) including experimental groups were fed for 28 days. The significant difference between the living weights was determined firstly as 278.64±5.61a; 268.94±2.80ab; 257.93±4.86b and 257.38±3.42b g in the Con, F, FCaD1, FCaD2 groups, respectively, at the end of the first week (P<0.05). This difference was seen to persist during the 3rd week of the trial. It was observed that this difference continued until the end of the 3rd week of the study. In the study, plasma Triglyceride (TG) amount was found to be in the form of 36.25±2.76b; 99.13±15.63a; 98.50±18.00a and 79.88±9.33ab mg.dl-1 in Con, F, FCaD1 and FCaD2 groups, respectively (P<0.05). The SCD-1 gene's liver expression levels were assessed as 11.83±2.08 (P<0.001); 5.31±1.40 (P<0.05) and 5.18±1.43 (P<0.05), respectively, compared to the Con group; SREBP-1c was determined as 2.11±0.37 (P<0.05); 3.41±1.20 (P<0.05) and 1.79±0.30 (P<0.05), respectively. In this study, it has been shown through the SREBP-1c and SCD-1 genes that calcium-vitamin D supplementation can have positive effects in a short time against lipogenesis induced by high fructose diet.

Bu çalışmada diyetle alınan yüksek miktarlardaki kalsiyum ve vitamin D’nin fruktozla indüklenmiş lipogenez üzerine olabilecek koruyucu etkileri moleküler ve biyokimyasal düzeyde araştırılmıştır. Kontrol grubu (Kon, n=8), Yüksek fruktozlu diyet grubu (F, n=8), Yüksek fruktozlu diyet+75 mg CaCO3 ve 26,4 IU vitamin D3 alan grup (FCaD1, n=8), Yüksek fruktozlu diyet+150 mg CaCO3 ve 52,8 IU vitamin D3 alan grup (FCaD2, n=8) olmak üzere 4 grup rat 28 gün boyunca beslenmiştir. Canlı ağırlıklar arasındaki anlamlı farklılık ilk olarak 1. hafta sonunda Kon, F, FCaD1, FCaD2 gruplarında sırasıyla 278,64±5,61a; 268,94±2,80ab; 257,93±4,86b ve 257,38±3,42b g olarak tespit edilmiştir (P<0,05). Bu farklılığın çalışmanın 3. haftasının sonuna kadar devam ettiği görülmüştür. Çalışmada plazma TG miktarının Kon, F, FCaD1 ve FCaD2 gruplarında sırasıyla 36,25±2,76b; 99,13±15,63a; 98,50±18,00a ve 79,88±9,33ab mg.dl-1 şeklinde olduğu görülmüştür (P<0,05). Karaciğerde SCD-1 geninin ekspresyon seviyeleri Kon grubuna göre sırasıyla 11,83±2,08 (P<0,001); 5,31±1,40 (P<0,05) ve 5,18±1,43 (P<0,05) olarak, SREBP-1c geninin ekspresyon seviyeleri ise sırasıyla 2,11±0,37 (P<0,05); 3,41±1,20 (P<0,05) ve 1,79±0,30 (P<0,05) olarak tespit edilmiştir. Yapılan bu çalışmada, yüksek fruktozlu diyet ile indüklenmiş lipogeneze karşı kalsiyum-vitamin D takviyesinin kısa sürede olumlu etkilerinin olabileceği SREBP-1c ve SCD-1 genleri üzerinden gösterilmiştir.

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