kvj Kocatepe Veterinary Journal 2147-6853 Afyon Kocatepe University 10.30607/kvj.1479289 Veterinary Obstetrics and Gynecology Veteriner Doğum ve Jinekoloji Evaluation of Erythrocytes Indices, Platelet Indices and Complete Blood Count in Feline Mammary Carcinomas Kedi Meme Karsinomlarında Eritrosit İndeksleri, Trombosit İndeksleri ve Tam Kan Sayımının Değerlendirilmesi https://orcid.org/0000-0002-8391-905X Kurban İbrahim İSTANBUL ÜNİVERSİTESİ-CERRAHPAŞA, VETERİNERLİK MESLEK YÜKSEKOKULU https://orcid.org/0000-0003-2530-1291 Günay Uçmak Zeynep ISTANBUL UNIVERSITY-CERRAHPASA, FACULTY OF VETERINARY MEDICINE, DEPARTMENT OF CLINICAL SCIENCES, DEPARTMENT OF OBSTETRICS AND GYNECOLOGY 09 30 2024 17 3 176 182 05 06 2024 07 24 2024 Copyright © 2008, Kocatepe Veterinary Journal 2008 Kocatepe Veterinary Journal

The aim of this study was to evaluate the differences in the platelet (PLT) indices, erythrocytes indices and complete blood count (CBC) parameters between the cats with mammary tumor and healthy queens. Also, the differences in above mentioned parameters are going to be investigated in cats with mammary tumors with regard to clinicopathological characteristics of the primary tumor. A total of 44 queens were included in the study. The study groups consisted of cats with mammary tumors (Group MT, n=22) and healthy cats presented for neutering (Group H, n=22). The ages of the cats were ranged between 5-15 years. The mean ages of the cats in Group MT was 10.77±0.80 and in Group H was 6.75±1.21 years. All data on CBC, thoracic radiography, clinical examination findings and histopathology were obtained from the the patient archive in the animal hospital automation system. While the highest red blood cell (RBC) and hemoglobulin (HGB) levels were observed in Group H, Group MT had the highest white blood cell (WBC), mean platelet volume (MPV) and plateletcrit (PCT) levels. The mean corpuscular volume (MCV) level in T1 was significantly lower than in T2-T3. The MCV level in G1-G2 tended to be higher compared to G3.In Group MT, CBC parameters were not significantly different related to the presence of metastasis, presence of ulceration and inflammation on the tumor. It was concluded that evaluation of CBC and PLT indices in cats with mammary tumors would be useful in understanding the hematological effects of the disease and tumor characteristics (tumor size and histological grade).

Bu çalışmanın amacı, meme tümörlü kediler ile sağlıklı dişi kediler arasındaki trombosit (PLT) indeksleri, eritrosit indeksleri ve tam kan sayımı (CBC) parametrelerindeki farklılıkları değerlendirmektir. Ayrıca meme tümörlü kedilerde yukarıda belirtilen parametrelerdeki farklılıklar, primer tümörün klinikopatolojik özellikleri açısından araştırılacaktır. Çalışmaya toplam 44 dişi kedi dahil edildi. Çalışma grupları, meme tümörlü kediler (Grup MT, n=22) ve kısırlaştırma için getirilen sağlıklı kedilerden (Grup H, n=22) oluşturuldu. Kedilerin yaşları 5-15 arasında değişiyordu. Grup MT'deki kedilerin yaş ortalaması 10,77±0,80, Grup H'deki kedilerin yaş ortalaması ise 6,75±1,21 yıldı. Tam kan sayımı, toraks radyografisi, klinik muayene bulguları ve histopatolojik incelemeye ilişkin tüm veriler, hayvan hastanesi otomasyon sistemindeki hasta arşivinden elde edildi. En yüksek kırmızı kan hücresi (RBC) ve hemoglobin (HGB) düzeyleri Grup H'de görülürken, Grup MT en yüksek beyaz kan hücresi (WBC), ortalama trombosit hacmi (MPV) ve platelekrit (PCT) düzeylerine sahipti. T1'deki ortalama korpüsküler hacim (MCV) seviyesi T2-T3'e göre anlamlı derecede düşüktü. G1-G2'deki MCV seviyesi G3'e kıyasla daha yüksek olma eğilimindeydi. Grup MT'de CBC parametreleri tümörde metastaz varlığı, ülserasyon ve inflamasyon varlığına bağlı olarak anlamlı farklılık göstermedi. Meme tümörlü kedilerde CBC ve PLT indekslerinin değerlendirilmesinin hastalığın ve tümör özelliklerinin (tümör boyutu ve histolojik derecesi) hematolojik etkilerinin anlaşılmasında faydalı olacağı sonucuna varıldı.

 Erythrocytes indices Female cat Mammary carcinoma Platelet indices Dişi kedi Eritrosit indeksleri Meme karsinomu Trombosit indeksleri Yoktur Yoktur Alan, E. M., Bamaç, Ö. E., & Koenhemsi, L. (2022). Evaluation of platelet count and platelet indices in cats and dogs diagnosed with lymphoma. Kocatepe Veterinary Journal, 15(3), 332-341. https://doi.org/10.30607/kvj.1133202 Avcı, B., Avcı, O., Solmaz, D., Yetişyiğit, T., & Burhan, T. (2017). Contribution of leukocyte platelet aggregates to development of thrombosis in patients with advanced cancer. Namık Kemal Medical Journal, 5(1), 7-15. Bailey, D. B. (2020). Paraneoplastic syndromes. In D. M. Vail, D. H. Thamm, & J. M. Liptak (Eds.), Small Animal Clinical Oncology (pp. 98-112). Elsevier. Buergy, D., Wenz, F., Groden, C., & Brockmann, M. A. (2012). Tumor-platelet interaction in solid tumors. International Journal of Cancer, 130(12), 2747-2760. https://doi.org/10.1002/ijc.27441 Coffelt, S. B., Wellenstein, M. D., & de Visser, K. E. (2016). Neutrophils in cancer: Neutral no more. Nature Reviews Cancer, 16(7), 431–446. https://doi.org/10.1038/nrc.2016.52 Contursi, A., Grande, R., Dovizio, M., Bruno, A., Fullone, R., & Patrignani, P. (2018). Platelets in cancer development and diagnosis. Biochemical Society Transactions, 46(6), 1517–1527. https://doi.org/10.1042/BST20180159 Da Silva Soares, E., Rocha, C. C., Valente, F. L., Dos Anjos, L. R. A., de Oliveira, F. L. D., de Oliveira Loures, C., Rocha, P. T., Castro, V. R., Sarandy, T.B. & Borges, A. P. B. (2023). Platelet count and MCHC as independent prognostic markers for feline mammary carcinomas. Research in Veterinary Science, 164, 105024. https://doi.org/10.1016/j.rvsc.2023.105024 Dagher, E., Abadie, J., Loussouarn, D., Campone, M., & Nguyen, F. (2019). Feline invasive mammary carcinomas: prognostic value of histological grading. Veterinary Pathology, 56(5), 660-670. https://doi.org/10.1177/0300985819846870 Elliott, J. (2014). Paraneoplastic syndromes in dogs and cats. In Practice, 36(9), 443-452. https://doi.org/10.1136/inp.g5826 Fridman, W. H., Zitvogel, L., Sautès-Fridman, C., & Kroemer, G. (2017). The immune contexture in cancer prognosis and treatment. Nature Reviews Clinical Oncology, 14, 717–734. https://doi.org/10.1038/nrclinonc.2017.101 Gaspar, B. L., Sharma, P., & Das, R. (2015). Anemia in malignancies: Pathogenetic and diagnostic considerations. Hematology, 20(1), 18-25. https://doi.org/10.1179/1607845414Y.0000000161 Goldschmidt, M.H., L. Pena, V. Zappulli (2017). Tumors of the mammary gland. In D.J. Meuten (Ed.), Tumors in Domestic Animals (pp. 723–765). Wiley-Blackwell. Günay Uçmak, Z., & Güvenç, K. (2019). Malign mammary tumors in female dogs: Evaluation of clinical and certain hematological parameters. Turkiye Clinics Journal of Veterinary Sciences, 10(2). https://doi.org/10.5336/vetsci.2019-71596 Günay Uçmak, Z., Koenhemsi, L., Ateş, F., Tarhan, D., Gürgen, H. Ö., Yildirim, F., Uçmak, M., Kırşan, İ., Ercan, A. M., & Or, M. E. (2023). Amounts of tissue magnesium and some trace elements in cats with mammary tumors related to various clinicopathological parameters. Journal of Trace Elements in Medicine and Biology, 79, 127246. https://doi.org/10.1016/j.jtemb.2023.127246 Gu, M., Zhai, Z., Huang, L., Zheng, W., Zhou, Y., Zhu, R., Shen, F., & Yuan, C. (2016). Pre-treatment mean platelet volume associates with worse clinicopathologic features and prognosis of patients with invasive breast cancer. Breast Cancer, 23(5), 752-760. https://doi.org/10.1007/s12282-015-0635-6 Hassan, B. B., Elshafae, S. M., Supsavhad, W., Simmons, J. K., Dirksen, W. P., Sokkar, S. M., & Rosol, T. J. (2017). Feline mammary cancer: Novel nude mouse model and molecular characterization of invasion and metastasis genes. Veterinary Pathology, 54, 32–43. https://doi.org/10.1177/0300985816650243 Hirota, T., Yonemaru, K., Hattori, M., Murakami, M., Sakai, H., & Hirata, A. (2024). Highly malignant endometrial stromal sarcoma in a cat. Journal of Comparative Pathology, 208, 11-14. https://doi.org/10.1016/j.jcpa.2023.10.011 Hristov, T., & Binev, R. (2018). Blood count in dogs with mammary gland carcinoma. Agricultural Science and Technology, 10(1), 44 – 47. https://doi.org/10.15547/ast.2018.01.011 Ito, T., Kadosawa, T., Mochızuki, M., Matsunaga, S., Nishımura, R., & Sasaki, N. (1996). Prognosis of malignant mammary tumor in 53 cats. Journal of Veterinary Medical Science, 58(8), 723-726. Ji, Y., Sheng, L., Du, X., Qui, G., & Su, D. (2015). Elevated platelet count is a strong predictor of poor prognosis in stage I non-small cell lung cancer patients. Platelets, 26(2), 138-142. https://doi.org/10.3109/09537104.2014.888547 Karateke, A., Kaplanoglu, M., & Baloglu, A. (2015). Relations of platelet indices with endometrial hyperplasia and endometrial cancer. Asian Pacific Journal of Cancer Prevention, 16(12), 4905-4908. https://doi.org/10.7314/APJCP.2015.16.12.4905 Klopfleisch, R. (2017). Feline mammary tumors (FMT). In R. Klopfleisch (Ed.), Veterinary Oncology Compact (1st ed., pp. 103-108). Springer Verlag GmbH. Koenhemsi, L., Uçmak, Z. G., Uçmak, M., & Or, M. E. (2020). Platelet indices in dogs and cats with pyometra. Revue Vétérinaire Clinique, 55(4), 147-150. https://doi.org/10.1016/j.anicom.2020.07.002 Köse, S. İ., Köse, A. M., Ürer, E. K., Bahan, O., Gözer, A., & Ambarcıoğlu, P. (2023). Diagnosis of transmissible venereal tumors in bitches—platelet indices are a remarkable marker?. Acta Scientiae Veterinariae, 51. https://doi.org/10.22456/1679-9216.132008 Küçükbekir, Ç. N., Günay Uçmak, Z., Kırşan, İ.& Tek, Ç. (2020). A case of feline fibroepithelial hyperplasia in a male cat. Journal of Istanbul Veterinary Sciences, 4(1), 8-12. https://doi.org/10.30704/http-www-jivs-net.691787 Lana, S., Rutteman, G. R., & Withrow, S. J. (2007). Tumors of the mammary gland. In S. J. Withrow & D. M. Vail (Eds.), Withrow and MacEwen’s Small Animal Clinical Oncology (4th ed., pp. 619–636). Saunders Elsevier. Ma, X., Wang, Y., Sheng, H., Tian, W., Qi, Z., Teng, F., & Xue, F. (2014). Prognostic significance of thrombocytosis, platelet parameters, and aggregation rates in epithelial ovarian cancer. The Journal of Obstetrics and Gynaecology Research, 40(1), 178-183. https://doi.org/10.1111/jog.12151 Mantas, D., Kostakis, I. D., Machairas, N., & Markopoulos, C. (2016). White blood cell and platelet indices as prognostic markers in patients with invasive ductal breast carcinoma. Oncology Letters, 12(2), 1610-1614. https://doi.org/10.3892/ol.2016.4760 Nascimento, C., & Ferreira, F. (2021). Tumor microenvironment of human breast cancer and feline mammary carcinoma as a potential study model. Biochimica et Biophysica Acta, Reviews on Cancer, 1876(1), 188587. https://doi.org/10.1016/j.bbcan.2021.188587 Nash, G. F., Walsh, D. C., & Kakkar, A. K. (2001). The role of the coagulation system in tumour angiogenesis. Lancet Oncology, 2(10), 608-613. https://doi.org/10.1016/S1470-2045(01)00518-6 Naess, I. A., Christiansen, S. C., Romundstad, P., Cannegieter, S. C., Rosendaal, F. R., & Hammerstrøm, J. (2007). Incidence and mortality of venous thrombosis: a population-based study. Journal of Thrombosis and Haemostasis, 5(4), 692-699. https://doi.org/10.1111/j.1538-7836.2007.02450.x Petrucci, G. N., Lobo, L., Queiroga, F., Martins, J., Prada, J., Pires, I., & Henriques, J. (2021). Neutrophil-to-lymphocyte ratio is an independent prognostic marker for feline mammary carcinomas. Veterinary and Comparative Oncology, 19(3), 482–491. https://doi.org/10.1111/vco.12686 Saran, K., Vidya, K., Seema, K., Prasad, A., & Prakash, J. (2022). Study of platelet indices and their role in evaluation of thrombocytopenia. Journal of Family Medicine and Primary Care, 11, 6236-6242. https://doi.org/10.4103/jfmpc.jfmpc_460_22 Thomas, R. (2015). Cytogenomics of feline cancers: advances and opportunities. Veterinary Science, 2, 246–258. https://doi.org/10.3390/vetsci2030246 Tvedten, H. (2022). Classification and laboratory evaluation of anemia. In M. B. Brooks, K. E. Harr, D. M. Seelig, K. J. Wardrop, & D. J. Weiss (Eds.), Schalm's Veterinary Hematology (pp. 198-208). Uçmak, Z. G., Uçmak, M., Tek, Ç., Koenhemsi, L., Bamaç, Ö. E., Gürel, A., & Yildar, E. (2018). Granulosa cell tumor in a spayed young queen. Journal of the Hellenic Veterinary Medical Society, 69(2), 1010-1015. https://doi.org/10.12681/jhvms.18022 Uçmak, Z. G., Koenhemsi, L., Uçmak, M., Or, M. E., Bamaç, Ö. E., Gürgen, H. Ö., & Yaramış, Ç. P. (2021). Evaluation of platelet indices and complete blood count in canine mammary tumors. Acta Scientiae Veterinariae, 49. https://doi.org/10.22456/1679-9216.114293 Üstündağ Budak, Y., Polat, M., & Huysal, K. (2016). The use of platelet indices, plateletcrit, mean platelet volume, and platelet distribution width in emergency non-traumatic abdominal surgery: a systematic review. Biochemia Medica, 26(2), 178—193. https://doi.org/10.11613/BM.2016.020 Varlotto, J., & Stevenson, M. A. (2005). Anemia, tumor hypoxemia, and the cancer patient. International Journal of Radiation Oncology, Biology, Physics, 63(1), 25-36. https://doi.org/10.1016/j.ijrobp.2005.04.049 Zappulli, V., Pena, L., Rasotto, R., Goldschmidt, M. H., Gama, A., Scruggs, J. L., & Kiupel, M. (2008). Volume 2: Mammary Tumors. In M. Kiupel (Ed.), Surgical Pathology of Tumors of Domestic Animals. Davis-Thompson DVM Foundation.