Characterization of Anogenital Distance and Its Relationship with Fertility in German Fawn × Hair Crossbred and Saanen Goats

Year 2024, , 89 - 94, 30.06.2024

Metehan Kutlu , Murat Durmuş

https://doi.org/10.30607/kvj.1421992

Abstract

In the presented study, it was aimed to reveal the characterization of anogenital distance (AGD) and its relationship with fertility in German Fawn × Hair crossbred and Saanen goats whose estrus was synchronized during the breeding season. 53 heads of German Fawn × Hair crossbred (75% German Pied and 25% Hair goat) and 55 heads of Saanen breed goats were used in the study. Before applying the sponge, the anogenital distances of all goats were measured and recorded with a digital caliper. For each breed, they were divided into two AGD categories: short AGD group and long AGD group. A sponge containing 60 mg medroxyprogesterone acetate was applied intravaginally to these goats on day 0, and the sponges were kept in the vagina for 13 days. On the thirteenth day of application, sponges were removed in all goats, and eCG was administered at a dose of 500 IU at the time of sponge removal. Goats on estrus were hand mated with one of the proven bucks (goat:buck ratio of 7:1). The parameters of estrus rate, pregnancy rate, kidding rate, multiple birth rate, litter size and male litter rate were calculated in all groups. After the statistical analysis, no significant difference was detected in the parameters monitored between the groups (p>0,05). It was concluded that anogenital distance did not make a difference on fertility in German Fawn × Hair crossbred and Saanen goats whose estrus was synchronized with progestagen applications during the breeding season. It is thought that more detailed studies are needed to clearly demonstrate the characterization of anogenital distance and its relationship with fertility in goats.

Keywords

Anogenital distance, breeding season, fertility, goat

Alman Alaca × Kıl melezi ve Saanen Irkı Keçilerde Anogenital Mesafenin Karekterizasyonu ve Fertilite ile İlişkisi

Abstract

Sunulan çalışmada, üreme mevsimi içerisinde östrüsları senkronize edilen Alman Alaca × Kıl melezi ve Saanen ırkı keçilerde anogenital mesafenin (AGM) karekterizasyonu ve fertilite ile ilişkisinin ortaya konulması amaçlanmıştır. Çalışmada 53 baş Alman Alaca x Kıl melezi (%75 Alman Alaca ve %25 Kıl keçisi) ve 55 baş Saanen ırkı keçi kullanıldı. Sünger uygulanmadan önce tüm keçilerin anogenital mesafeleri dijital kumpasla ölçülerek kaydedildi. Her ırk için kısa AGM grubu ve uzun AGM grubu olmak üzere iki AGM kategorisine ayrıldı. Bu keçilere 0. gün intravajinal 60 mg medroksiprogesteron asetat içeren sünger uygulandı, süngerler 13 gün süreyle vajinada tutuldu. Tüm keçilerde uygulamanın on üçüncü günü süngerler çıkarıldı ve sünger çıkarılması anında 500 IU dozda eCG uygulandı. Östrüste olduğu tespit edilen keçiler elde aşım yöntemiyle (teke/keçi oranı: 1/7) fertil tekelerden bir tanesi ile çiftleştirildi. Teke katımından sonraki 40. günde çiftleşen hayvanlara ultrasonografik gebelik muayenesi yapıldı. Tüm gruplarda östrüs oranı, gebelik oranı, doğum oranı, çoklu doğum oranı, yavru verimi ve erkek yavru oranı parametreleri hesaplandı. Yapılan istatistiksel analiz sonrası gruplar arasında takip edilen parametreler arasında önemli bir fark saptanmadı (p>0,05). Sonuç olarak üreme mevsimi içerisinde progestagen uygulamaları ile östrüsları senkronize edilen Alman Alaca x Kıl melezi ve Saanen ırkı keçilerde anogenital mesafenin fertilite üzerine farklılık yaratmadığı görüldü. Keçilerde anogenital mesafenin karekterizasyonu ve fertilite ile ilişkisinin açıkça ortaya konulabilmesi için daha ayrıntılı çalışmalara ihtiyaç olduğu düşünülmektedir.

Keywords

Anogenital mesafe, fertilite, keçi, üreme sezonu

Ethical Statement

Çalışma Çukurova Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu’nun 20.07.2023 tarih ve 5/14 sayılı onayı ile gerçekleştirildi.

Supporting Institution

yok

References

• Akbarinejad V, Gharagozlou F, Vojgani M, Shourabi E, & Makiabadi MJM. Inferior fertility and higher concentrations of anti-müllerian hormone in dairy cows with longer anogenital distance. Domestic Animal Endocrinology, 2019; 68: 47-53. doi: 10.1016/j.domaniend.2019.01.011 Bánszegi O, Szenczi P, Dombay K, Bilkó Á, & Altbäcker V. Anogenital distance as a predictor of attractiveness, litter size and sex ratio of rabbit does. Physiology and Behavior, 2012; 105(5): 1226-1230. doi: 10.1016/j.physbeh.2012.01.002
• Beci B, Van Eetvelde M, Vanlommel L, & Opsomer G. The anogenital ratio as an indicator of reproductive performance in dairy heifers. Theriogenology, 2023; 210: 94-100. doi: 10.1016/j.theriogenology.2023.07.019
• Bowman CJ, Barlow NJ, Turner KJ, Wallace DG, & Foster PM. Effects of in utero exposure to finasteride on androgen-dependent reproductive development in the male rat. Toxicological Sciences, 2003; 74(2): 393-406. doi: 10.1093/toxsci/kfg128
• Carrelli JE, Gobikrushanth M, Corpron M, Rajesh I, Sandberg W, Colazo MG, Ahmadzadeh A, Oba M, & Ambrose DJ. Relationship of anogenital distance with fertility in nulliparous Holstein heifers. Journal of Dairy Science, 2021; 104(7): 8256-8264. doi: 10.3168/jds.2020-19940
• Carrelli JE, Gobikrushanth M, Corpron M, Sandberg W, Rajesh I, Ahmadzadeh A, Oba M, & Ambrose DJ. Associations between anogenital distance and measures of fertility in lactating North American Holstein cows: A validation study. Journal of Dairy Science, 2022; 105(7): 6339-6352. doi: 10.3168/jds.2021-20827
• Dean A, Smith LB, Macpherson S, & Sharpe RM. The effect of dihydrotestosterone exposure during or prior to the masculinization programming window on reproductive development in male and female rats. International Journal of Andrology, 2012; 35(3): 330-339. doi: 10.1111/j.1365-2605.2011.01236.x
• Drickamer LC, Arthur RD, & Rosenthal TL. Conception failure in swine: importance of the sex ratio of a female's birth litter and tests of other factors. Journal of Animal Science, 1997; 75(8): 2192-6. doi: 10.2527/1997.7582192x
• Feknous N, Belabbas R, Berbar A, & Zenia S. Study of anogenital distance in rabbits: effect on sexual behavior and litter size biological components. Veterinarija ir Zootechnika, 2021; 79(1): 65-71.
• Gobikrushanth M, Bruinjé TC, Colazo MG, Butler ST, & Ambrose DJ. Characterization of anogenital distance and its relationship to fertility in lactating Holstein cows. Journal of Dairy Science, 2017; 100(12): 9815-9823. doi: 10.3168/jds.2017-13033 Gobikrushanth M, Purfield DC, Kenneally J, Doyle RC, Holden SA, Martinez PM, Canadas ER, Bruinjé TC, Colazo MG, Ambrose DJ, & Butler ST. The relationship between anogenital distance and fertility, and genome-wide associations for anogenital distance in Irish Holstein-Friesian cows. Journal of Dairy Science, 2019; 102(2): 1702-1711. doi: 10.3168/jds.2018-15552
• Grala TM, Price MD, Kuhn-Sherlock B, Burke CR, & Meier S. Investigating anogenital distance and antral follicle count as novel markers of fertility within a herd of cows with positive or negative genetic merit for fertility traits. Journal of Dairy Science, 2021; 104(12): 12939-12952. doi: 10.3168/jds.2020-19948
• Hotchkiss AK, Lambright CS, Ostby JS, Parks-Saldutti L, Vandenbergh JG, & Gray LE, Jr. Prenatal testosterone exposure permanently masculinizes anogenital distance, nipple development, and reproductive tract morphology in female Sprague-Dawley rats. Toxicological Sciences, 2007; 96(2): 335-45. doi: 10.1093/toxsci/kfm002 Kutlu M, Alkan H, Doğan H, & Dinç DA. The effect of carprofen treatment on reproductive parameters following progestagen administration in lactating German Fawn × Hair crossbred goats during the transitional period. Large Animal Review, 2022; 28: 131-135.
• Lamm CG, Hastie PM, Evans NP, & Robinson JE. Masculinization of the distal tubular and external genitalia in female sheep with prenatal androgen exposure. Veterinary Pathology, 2012; 49(3): 546-51. doi: 10.1177/0300985811419533
• Macleod DJ, Sharpe RM, Welsh M, Fisken M, Scott HM, Hutchison GR, Drake AJ, & van den Driesche S. Androgen action in the masculinization programming window and development of male reproductive organs. International Journal of Andrology, 2010; 33(2): 279-87. doi: 10.1111/j.1365-2605.2009.01005.x
• Makiabadi MJM, Akbarinejad V, Heidari F, Gharagozlou F, & Vojgani M. Greater reproductive performance in holstein dairy cows with moderate length of anogenital distance at first service postpartum. Iranian Journal of Veterinary Medicine, 2022; 16(1): 46-56. doi: 10.22059/IJVM.2020.309538.1005125
• Manikkam M, Crespi EJ, Doop DD, Herkimer C, Lee JS, Yu S, Brown MB, Foster DL, & Padmanabhan V. Fetal programming: prenatal testosterone excess leads to fetal growth retardation and postnatal catch-up growth in sheep. Endocrinology, 2004; 145(2): 790-798. doi: 10.1210/en.2003-0478
• Mendiola J, Roca M, Mínguez-Alarcón L, Mira-Escolano M-P, López-Espín JJ, Barrett ES, Swan SH, & Torres-Cantero AM. Anogenital distance is related to ovarian follicular number in young Spanish women: a cross-sectional study. Environmental Health, 2012; 11(1): 90. doi: 10.1186/1476-069X-11-90
• Mira-Escolano MP, Mendiola J, Mínguez-Alarcón L, Melgarejo M, Cutillas-Tolín A, Roca M, López-Espín JJ, Noguera-Velasco JA, & Torres-Cantero AM. Longer anogenital distance is associated with higher testosterone levels in women: a cross-sectional study. An International Journal of Obstetrics and Gynaecology, 2014; 121(11): 1359-64. doi: 10.1111/1471-0528.12627 Özkan H, & Yakan A. Genomic selection in animal breeding: past, present. Lalahan Hayvancılık Araştırma Enstitüsü Dergisi, 2017; 57: 112-117.
• Salazar-Martinez E, Romano-Riquer P, Yanez-Marquez E, Longnecker MP, & Hernandez-Avila M. Anogenital distance in human male and female newborns: a descriptive, cross-sectional study. Environmental Health, 2004; 3(1): 8. doi: 10.1186/1476-069X-3-8 Sathyanarayana S, Beard L, Zhou C, & Grady R. Measurement and correlates of ano-genital distance in healthy, newborn infants. International Journal of Andrology, 2010; 33(2): 317-23. doi: 10.1111/j.1365-2605.2009.01044.x
• Shourabi E, Hakimi H, Baqeri A, Gharagozlou F, Vojgani M, Foroutannejad M, Baghbanani RH, Mobedi E, & Akbarinejad V. Evidence that Murciano-Granadina does with longer anogenital distance are more fertile and prolific and produce heavier and male-biased litters. Anim Reprod Sci, 2022; 244: 107047. doi: 10.1016/j.anireprosci.2022.107047
• Wu Y, Zhong G, Chen S, Zheng C, Liao D, & Xie M. Polycystic ovary syndrome is associated with anogenital distance, a marker of prenatal androgen exposure. Human Reproduction, 2017; 32(4): 937-943. doi: 10.1093/humrep/dex042
• Zehr JL, Gans SE, & McClintock MK. Variation in reproductive traits is associated with short anogenital distance in female rats. Developmental Psychobiology, 2001; 38(4): 229-238. doi: 10.1002/d