Farelerde Parasetamol ile İndüklenen Akut Karaciğer Hasarı Üzerine Narın (Punica granatum L.) Etkileri

Year 2016, Volume: 9 Issue: 4, 273 - 280, 01.12.2016

Mehmet Ali Erfidan Esra Küpeli Akkol Alper Sevimli Turan Civelek

Abstract

Parasetamol toksisitesinin akut karaciğer hasarının en önemli nedeni olduğu rapor edilmiştir. Asetaminofen toksikasyonunda antidot olarak kullanılabilen müstahzarlar bulunmakla birlikte, bazı olgularda bu ilaçlar etkisiz kalabilmektedir. Bu çalışmanın amacı, akut karaciğer hasarı modelinde tam meyve liyofilize Punica granatum L. ekstresinin koruyucu etkinliğini ve yan etkilerini ortaya koymaktır. Çalışmada 80 Swiss Albino fare kullanıldı. Araştırma iki ana grup üzerinde yürütüldü. Yan etki grubundaki farelere 100, 200 ve 400 mg/kg dozunda nar ekstresi verildi. Deneme grubuna ise tek doz parasetamol ile birlikte, eş zamanlı olarak, bahsedilen dozlarda nar ekstresi uygulandı. Biyokimya analiz sonuçları parasetamol ile birlikte verilen liyofilize ekstrenin serum AST, ALT, TP ve TG düzeylerinde önemli oranda bir azalmaya neden olduğunu ortaya koydu. Deneme grubunda verilen nar ekstresi ise RBC, HB, HCT ve LENF konsantrasyonlarında bir düşüşe yol açtı. Histopatoloji analiz sonuçları tek başına verilen ekstrenin hafif-orta derecede karaciğer hasarına yol açabileceğini ortaya koydu. Parasetamol ile birlikte eş zamanlı verilen nar ekstresinin, biyokimya sonuçlarına göre, 200 mg/kg ve histopatoloji sonuçlarına göre ise 400 mg/kg dozlarında karaciğer üzerinde koruyucu etkinliği olabileceği belirlendi. Ölçülen AoA düzeyleri, deneme grubu için, verilen ekstrenin antioksidan aktivitesine vurgu yapmaktadır. Yan etki grubundan elde edilen sonuçlar ise farelerde kullanılan tam meyve liyofilize nar suyu ekstresinin antioksidan aktivite etkisini azaltma eğiliminde olabileceğini gösterdi.

Keywords

Asetaminofen, Antioksidan aktivite, Hepatotoksisite, Hepatoprotektif aktivite, Kınagiller

Effects of Pomegranate (Punica granatum L.) on Paracetamol Induced Acute Hepatic Damage in Mice

Abstract

The most common cause of acute liver failure has been reported as paracetamol toxicity. There are some drugs that can be used as an antidote for acetaminophen toxicity, but in some cases these drugs are ineffective. The purpose of this study is to identify protective and side effects of lyophilized extract’s from Punica granatum L. in acute liver injury model. In our study, 80 Swiss Albino mice were used. The research was conducted on two main groups. The mice in the side effect group were given pomegranate extracts at 100, 200 and 400 mg/kg doses. The trial group were administered mentioned doses of extracts and a single dose of paracetamol simultaneously. The results gained from biochemistry analysis demonstrated that lyophilized extract given along with paracetamol caused a significant decrease in serum AST, ALT, TP and TG levels. The pomegranate extract given to the experiment group led to a decrease in RBC, HB, HCT and LYMP levels. Histopathological evaluation demonstrated that the extract alone may cause liver injury ranging from mild to moderate. Yet the extract given simultaneously with paracetamol particularly may have a protective effect on liver in a certain dose of 200 mg/kg according to biochemistry results and in a certain dose of 400 mg/kg according to histopathological analysis. Measured AoA levels emphasize the antioxidant activity of the extract for trial group. The results obtained from the side effect group have emphasized possible reducing effect of antioxidant activity of lyophilized extract in mice.

Keywords

Acetaminophen, Antioxidant activity, Hepatotoxicity, Hepatoprotective activity, Lythraceae

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