Giardia duodenalis ile Doğal Enfekte Kuzularda Serum 25 (OH) D3 Seviyeleri

Year 2019, Volume: 12 Issue: 1, 71 - 74, 31.03.2019

Güzin CAMKERTEN Hasan ERDOĞAN Deniz Alıç URAL İlker CAMKERTEN Songül ERDOĞAN Kerem URAL

https://doi.org/10.30607/kvj.492805

Cited By: 1

Abstract

Giardia duodenalis kuşlar, reptiller ve
bir çok memeli hayvanı enfekte edebilen binükleuslu flagellalı bir
protozoondur. Söz konusu patojen insanlarda ve çiftlik hayvanlarında başta
olmak üzere şekillenen ishalin en önemli nedenleri arasında yer almaktadır.
Çalışmada Giardia duodenalis ile
doğal enfekte kuzularda serum 25-hidroksi vitamin D3 seviyelerindeki
değişimlerin belirlenmesi amaçlanmıştır. Araştırmanın hayvan materyalini Aydın
bölgesinde bulunan yaşları 15-35 günlük arasında her iki cinsiyetten sakız ırkı
Giardia duodenalis ile doğal enfekte
(n=30), benzer yaş aralığındaki aynı ırktan (n=15) ve her iki cinsiyetten
sağlıklı kuzular oluşturdu. Enfekte ve sağlıklı hayvanlardan dışkı örnekleri
rektal tuşe yöntemi ile steril dışkı örnekleri, serum 25-hidroksi vitamin D3
seviyelerinin belirlenmesi amacı ile de tekniğine uygun olarak v.jugularisden
toplamda 5 ml olacak şekilde kan örnekleri alındı. Dışkı örneklerinden direkt
mikroskobik bakı yöntemi ile giardia taraması yapıldı. Elde edilen serum
örneklerinden Savant marka flöresan immunoassay cihazı ile 25-OH-D seviyeleri
ölçüldü. İstatistiksel analizde Mann-Whitney U testinden yararlanıldı. Sağlıklı
ve doğal enfekte hayvanlara ait 25-hidroksi vitamin D3 seviyeleri arasında
istatistiksel (p<0,05) anlamlı değişimlerin olduğu belirlendi.

Keywords

Giardiasis, kuzu, 25-hidroksi vitamin D3

Levels of Serum 25 (OH) D3 in Naturally Infected Lambs With Giardia duodenalis

Abstract

Giardia duodenalis is a binuclear flagellated protozoan that can infect birds, reptiles and many mammals. The pathogen is one of the most important causes of diarrhea in humans and farm animals. The aim of this study was to determine the changes in serum 25-hydroxyvitamin D3 levels in naturally infected lambs with Giardia duodenalis. The animal material of the study was used Sakız sheep which had naturally infected with Giardia duodenalis between 15-35 days of age from the Aydın region (n = 30), healthy lambs in the same age range (n = 15) and both sexes. Sterile stool samples with the method of rectal touching of fecal samples from infected and healthy animals, blood samples were obtained in a total of 5 ml of v.jugularis according to the technique in order to determine serum 25-hydroxyvitamin D3 levels. Giardia screening was performed by direct microscopic examination of stool samples. 25-OH-D levels were measured with Savant brand fluorescent immunoassay device from the obtained serum samples. Mann-Whitney U test was used in statistical analysis. Statistically significant changes were observed in 25-hydroxyvitamin D3 levels of healthy and natural infected animals (p <0.05).

Keywords

Giardiasis, Lamb, 25-hydroxy vitamin D3

References

• Astiazaran-Garcia H, Lopez-Teros V, Valencia ME, Vazquez-Ortiz F, Sotelo-Cruz N, Quihui-Cota L. Giardia lamblia infection and its implications for vitamin A liver stores in school children. Annals of Nutrition and Metabolism. 2010;57(3-4): 228-233.
• Aytuğ CN, Alaçam E, Özkoç Ü, Yalçın BC. Koyun-Keçi Hastalıkları ve Yetiştiriciliği, İstanbul: Teknografik Matbaası, 1990; 212-213.
• Baljer G, Wieler L. Ätiologie. Pathogenese und Immunprophylaxe der neonatalen Durchfallerkrankungen der Kälber. Vet 1989; 5: 18-26.
• Bilal T. Koyun-Keçilerin İç Hastalıkları ve Beslenmesi. İstanbul Üniversitesi Yayın No: 4593, İstanbul: Veteriner Fakültesi Yayın No: 24, 2005.
• Buret AG. Pathophysiology of enteric infections with Giardia duodenalis. Parasite. 2008 ;15(3):261-265.
• Cordingley FT, Crawford GPM. Giardia infection causes vitamin B12 deficiency. Australian and New Zealand journal of medicine. 1986; 16(1): 78-79.
• Dignass AU. Mechanisms and modulation of intestinal epithelial repair. Inflamm Bowel Dis. 2001;7: 68 –77.
• Gaydos J. Giardia and Wildlife South Coop Wild Dis St Br 1998; 14:2.
• Geurden T, Pohleb H, Sarrea C, Dreesena L, Vercruyssea J, Claerebouta E. The efficacy of a treatment with fenbendazole against an experimental Giardia duodenalis infection in lambs. Small Rum Res. 2011; 96(2-3):211-215.
• Geurden T, Vercruysse J, Claerebout E. Is giardia a significant pathogen in production animals?. Exp Parasitol. 2009; 124:98-106. Gökçe E, Ünver A, Erdoğan HM. İshalli neonatal kuzularda enterik patojenlerin belirlenmesi. Kafkas Univ Vet Fak Derg.2010; 16:717-722.
• Hall GA, Jones PW, Morgan JH. Calf diarrhoea. Bovine Medicine, In: Andrews AH. (Editors). Diseases and Husbuandry of Cattle. Berlin: Blackwell, 1996; 154-180.
• Hassan V, Hassan S, Seyed-Javad P, Ahmad K, Asieh H, Maryam S, Farid F, Siavash A. Association Between Serum 25 (OH) Vitamin D Concentrations and Inflammatory Bowel Diseases (IBDs) Activity. Medical Journal of Malaysia. 2013; 68(1): 34-38.
• Kong J, Zhang Z, Musch MW, Ning G, Sun J, Hart J, Bissonnette M, Li YC. Novel role of the vitamin D receptor in maintaining the integrity of the intestinal mucosal barrier. Am J Physiol Gastrointest Liver Physiol. 2008; 294(1):208-16.
• Lim WC, Hanauer SB, Li YC. Mechanisms of Disease: Vitamin D and Inflammatory Bowel Disease. Nature Clinical Practice Gastroenterology and Hepatology.2005; 2(2):103-111.
• Lim WC, Hanauer SB, Li YC. Mechanisms of disease: vitamin D and inflammatory bowel disease. Nature Clin Pract Gastroenterol Hepatol. 2005;2: 308 –315.
• Loftus EV Jr. Clinical epidemiology of inflammatory bowel disease: incidence, prevalence, and environmental influences. Gastroenterology. 2004; 126: 1504 –1517.
• Lunn B, Northrop A. Symposium on ‘parasitism and protein and energy metabolism and animals’. The impact of gastrointestinal parasites on protein-energy malnutrition in man. Proc Nutr Soc.1992;52:101–111.
• Nerich V, Jantchou P, Boutron-Ruault MC, Monnet E, Weill A, Vanbockstael V, Auleley GR, Balaire C, Dubost P, Rican S, Allemand H, Carbonnel F. Low Exposure to Sunlight is a Risk Factor for Crohn’a Disease. Alimentary Pharmacology and Therapeutics. 2011; 33(8): 940-945.
• Nicholson I, Dalzell AM, El-Matary W. Vitamin D as a Therapy for Colitis: A Systematic Review. Journal of Crohn’s and Colitis. 2012; 6(4): 405-411.
• Olson ME, McAllister TA, Deselliers L, Morck DW, Cheng KJ, Buret AG, Ceri H. Effects of Giardiasis on production in a domestic ruminant (lamb) model. Am J Vet Res. 1995; 56:1470-1474.
• Radostits OM, Gay CC, Hinchcliff KW, Constable PD. Veterinary Medicine, 10th Edition, Philadelphia, USA: Saunders Elsevier Ltd, 2007.
• Read C, Walters J, Robertson I, Thompson R. Correlation between genotype of Giardia duodenalis and diarrhoea. Int J Parasitol. 2002;32:229–231.
• Robertson LJ. Giardia and Cryptosporidium infections in sheep and goats: A review of the potential for transmission to humans via environmental contamination. Epidemiol Infect. 2009; 137:913-921.
• Ruest N, Couture Y, Faubert GM, Girard C. Morphological changes in the jejunum of calves naturally infected with giardia spp. and Cryptosporidium spp. Vet Parasitol. 1997; 69:177-186.
• Sweeny JP, Jacobson C, Robertson I, Ryan UM. Carcass productivity consequences of trichostrongylid and protozoan parasites in Merino x Suffolk prime lambs in the South West of Western Australia. Conference proceedings: Melbourne, Australia: The International Congress of parasitology (ICOPA); 2010.
• Talachian E, Bidari A, Noorbakhsh S, Tabatabaei A, Salari F. Serum levels of vitamins A and D, and zinc in children with acute diarrhea: A cross-sectional study. Medical journal of the Islamic Republic of Iran. 2015; 29:200-207.
• Ulitsky A, Ananthakrishnan AN, Naik A, Skaros S, Zadvornova Y, Binion DG, Issa M. Vitamin D Deficiency in Patients with Inflammatory Bowel Disease. Journal of Parenteral and Enteral Nutrition. 2011;35(3): 308-316.
• Ural K, Aysul N, Voyvoda H, Ulutas B, Aldemir OS, Eren H. Single dose of secnidazole treatment against naturally occuring Giardia duodenalis infection in Sakiz lambs. Revista MVZ Córdoba. 2014;19(1):4023-4032.
• Voloc A. Study on Vitamin D and nutritional status in children and adolescents with helminthiases in central Moldova.Curierul medical. 2015; 58(6):22-29.