Investigation of Sox2, ß-III Tubulin and Nestin Expressions in Neuropsheres Differentiated from Bovine Adipose Derived Mesenchymal Stem Cells by Immunofluorescence Staining

Year 2019, Volume: 12 Issue: 3, 336 - 342, 30.09.2019

Özlem Özden Akkaya , Tayfun Dikmen , Shah Nawaz

https://doi.org/10.30607/kvj.594550

Cited By: 3

Abstract

Stem cell therapy can
be an advantageous tool for non treatable diseases and has become a theme
research for a better solution to many neurodegenerative cases. Despite of
being very common in pet animals and equines in the field of veterinary
medicine; stem cell based practises in bovines yet remain very limited. In the
following study, Bovine adipose derived stem cells (BASCs) were isolated and
differentiated into neurospheres. The potential expressions of neuron-specific
markers β-III Tubulin, Nestin and Sox2 were demonstrated by immunofluorescence
staining on these neurospheres. The outcomes of the study may provide a better
understanding to the neurogenic potential of BASCs and can be a footstep for
developing new therapeutical approaches for neurodegenerative diseases and
syndroms in bovines.

Keywords

Adipose, Bovine, Nestin, Neurosphere, Sox2

Sığır Yağ Dokusundan İzole Edilen Mezenkimal Kök Hücrelerden Farklılaştırılan Nörosferlerde Sox2, ß-III Tubulin ve Nestin Ekspresyonlarının İmmunfloresan Boyama Yöntemi ile İncelenmesi

Abstract

Kök hücre tedavisi,
pek çok tedavisi olmayan hastalıkta olduğu gibi çoğu nörodejeneratif hastalık
ve sendromda da daha iyi tedavi protokollerinin geliştirilmesi için önemli bir
çalışma alanı haline gelmiştir. Kök hücrelerin klinik kullanımı pet hayvanları
ve atlarda her ne kadar yaygınlaşmaya başlasa da sığırlarda kök hücre kullanımı
hala oldukça sınırlıdır. Bu çalışmada sığır yağ dokusu kaynaklı mezenkimal kök
hücreler izole edilmiş ve bu hücrelerden nörosfereldesi gerçekleştirilmiştir.
Elde edilen nörosferlerin nöronspresifik belirteçler olan ß-III Tubulin, Nestin
ve Sox2 ekspresyonları immunfloresan boyama tekniği ile değerlendirilmiştir.
Çalışmadan elde edilecek sonuçlar sığır yağ dokusu kaynaklı mezenkimal kök
hücrelerin nörojenik potansiyelinin daha iyi anlaşılması açısından önemli
olmakla birlikte bu sonuçlar sığırlarda nörojenik defektlerde kullanılabilecek
potansiyel tedavi yaklaşımlarının geliştirilmesi hususunda da yol gösterici
olacaktır. 

Keywords

Nestin, Nörosfer, Sığır, Sox2, Yağ Dokusu

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