An Immunohistochemical Investigation of The Effect of Sambucus Nigra on Chymase-, Tryptase- and Ghrelin- Positive Cells in Rat Lung

Year 2022, Volume: 15 Issue: 2, 209 - 216, 30.06.2022

Tuğrul ERTUĞRUL Gökçen SEVİLGEN

https://doi.org/10.30607/kvj.1070754

Abstract

Sambucus nigra (S.nigra) is used in the treatment of many diseases and disorders thanks to its antioxidant, anticarcinogenic, immunostimulating, antiallergic, antiviral, and antibacterial properties. Ghrelin has anti-inflammatory effect on oxidative damage in various organs and cell types. The aim of this study was to immunohistochemically examine the chymase, tryptase, and ghrelin in rat lung after S. nigra administration. A total of 16 male rats were used in the study. The rats were assigned to two groups, control and S. nigra. 1st control group (n=8): No application was made. 2nd S. nigra group (n=8): S. nigra extract was administered at 15 mg/kg by oral gavage for 14 days. Tryptase, chymase, and ghrelin-positive cells were found in the lung tissue in a spindle-shaped, round, or oval shape. When the groups were evaluated within themselves, a significant increase in the number of tryptase, chymase, and ghrelin positive cells was observed in the S. nigra treated group. This study showed that S. nigra, which has an immunomodulatory and antioxidant effect, increases the expression of chymase-, tryptase- and ghrelin-positive cells in the lungs. Additionally, based on our findings, it can be said that mast cells can produce, store and release ghrelin.

Keywords

Chymase, ghrelin, lung, Sambucus nigra, tryptase

Sıçan Akciğerinde Sambucus Nigra’nın Kimaz, Triptaz ve Ghrelin Pozitif Hücrelerin Üzerindeki Etkisinin İmmünohistokimyasal Olarak İncelenmesi

Abstract

Sambucus nigra (S. nigra) antioksidan, antikanserojenik, immün sistemi uyarıcı, antialerjik, antiviral ve antibakteriyel özellikleri sayesinde birçok hastalık ve rahatsızlığın tedavisinde kullanılmaktadır. Ghrelin, çeşitli organlarda ve hücre tiplerinde oksidatif hasar üzerinde anti-inflamatuar etkilere sahiptir. Bu çalışmanın amacı, S. nigra uygulamasından sonra sıçan akciğerinde kimaz, triptaz ve ghrelin immünopozitif hücrelerinin immünohistokimyasal olarak incelenmesidir. Çalışmada toplam 16 erkek sıçan kullanıldı. Sıçanlar kontrol ve S. nigra olmak üzere iki gruba ayrıldı. 1. kontrol grubu (n=8): herhangi bir uygulama yapılmadı. 2. S. nigra grubu (n=8): S. nigra ekstresi 14 gün süreyle oral gavaj yoluyla 15 mg/kg dozunda uygulandı. Akciğer dokusunda yuvarlak, oval veya mekik şeklinde triptaz, kimaz ve ghrelin pozitif hücreler bulundu. İki grup kendi içinde değerlendirildiğinde S. nigra uygulanan grupta triptaz, kimaz ve ghrelin pozitif hücre sayısında önemli artış gözlendi. Bu çalışma, immünomodülatör ve antioksidan etkiye sahip olan S. nigra'nın akciğerde kimaz, triptaz ve ghrelin pozitif hücrelerin ekspresyonunu arttırdığını göstermiştir. Ayrıca mast hücrelerinin diğer bağışıklık hücreleri gibi ghrelin üretebildiği, depolayabildiği ve serbest bırakabildiği açıklanmaya çalışılmıştır.

Keywords

Akciğer, ghrelin, kimaz, Sambugus nigra, triptaz

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